A High-Quality Draft Genome Sequence of Colletotrichum gloeosporioides sensu stricto SMCG1#C, a Causal Agent of Anthracnose on Cunninghamia lanceolata in China

Abstract

Colletotrichum has a broad host range and causes major yield losses of crops. The fungus Colletotrichum gloeosporioides is associated with anthracnose on Chinese fir. In this study, we present a high-quality draft genome sequence of C. gloeosporioides sensu stricto SMCG1#C, providing a reference genomic data for further research on anthracnose of Chinese fir and other hosts.

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

“…Based on the genome draft sequence of C. gloeosporioides s.s. SMCG1#C [ 20 ], the CgMCK1 gene replacement constructs were established using the overlap polymerase chain reaction (PCR) method as described [ 13 ]. Firstly, the upstream (~1.5 kb) and downstream (~1.5 kb) flanking sequences were amplified with primer set CgMCK1_F1/R1 and CgMCK1_F2/R2, respectively.…”

Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence

“…For the genus-wide maximum likelihood tree of selected Colletotrichum species, BUSCO was run on the genome assemblies of C. trifolii (RYZW01000000) , C. sidae (QAPF01000000) , C. orbiculare (AMCV02000000) and C. spinosum (QAPG01000000) 24 , C. fructicola (ANPB00000000.1) 19 , C. gloeosporioides (QFRH00000000) 27 , C. chlorophyti (MPGH01000000) 53 , C. orchidophilum (MJBS00000000.1) 54 , C. salicis (JFFI00000000.1) , C. fioriniae (JARH00000000.1) , C. simondsii (JFBX00000000.1) , C. nymphaea (JEMN00000000.1) 28 , C. sublineola (JMSE00000000.1) 29 , C. graminicola (ACOD00000000.1) 18 , C. incanum (JTLR01000000) 17 , C. tofieldiae (LFIV01000000) 37 , C. lentis (NWBT01000000) 21 , C. tanaceti (PJEX00000000) 26 , C. higginsianum (LTAN01000000) 33 and Fusarium oxysporum (GCF_000149955.1) 55 to identify highly conserved, single copy eukaryote genes (eukaryote_odb9). Sequences of orthologs from 254 single copy genes that were identified as non-duplicated in all the tested genomes were aligned using MAFFT and trimmed using trimAl as described above.…”

“…With the advent of affordable high throughput genome sequencing technologies, the genomes of multiple members of the Colletotrichum genus of plant pathogenic fungi have been sequenced and released 17–27 . Sequenced genomes have included strains belonging to different species complexes, which comprise closely related species that are phylogenetically distinct from other members of the same genus.…”

Colletotrichum Gloeosporioides and its Hosts: A Scientometric Analysis

“…C. gloeosporioides is capable of infecting at least 1,000 hosts, including the main commercial fruit species such as papaya, mango, guava and avocado [3]. However, the effects of the environment can intervene in the development of pathogens, thus, knowledge of environmental variations can contribute to disease management strategies [4,5]. The development of C. gloeosporioides occurs due to high temperature and relative humidity, where the conidia are released and disseminated at that moment when the clots are moist.…”