A high proportion of the Vδ1+ synovial fluid γδ T cells in juvenile rheumatoid arthritis patients express the very early activation marker CD69, but carry the high molecular weight isoform of the leucocyte common antigen (CD45RA)

Abstract

A high proportion of the V51+ synovial fluid y5 T cells in juvenile rheumatoid arthritis patients express the very early activation marker CD69, but carry the high molecular weight isoform of the leucocyte common antigen (CD45RA) SUMMARYWe have previously shown that y6 T cells in the synovial compartment of patients with juvenile rheumatoid arthritis (JRA) express activation antigens (CD69 and HLA-DR) and that they are predominantly of the V(51 subset. In this study we have analysed the expression ofactivation antigens (CD69 and HLA-DR) and different isoforms of the leucocyte common antigen (CD45RO and CD45RA) on the V5I and the V52 subsets of y^ T cells in paired samples of synovial fluid and peripheral blood of nine patients with JRA, and in the peripheral blood of five children with idiopathie seoliosis. In the synovial fluid of children with JRA, there were significantly more V(5I+CD69+ and V52+CD69+ cells compared with the peripheral blood ofthe same patients. In contrast, however, in the synovial fluid the \8\ and the \62 subsets differed with respect to the expression of the two isoforms of the leucocyte common antigen. The majority of the V51 + cells expressed the high molecular weight isoform (CD45RA+) while most ofthe V(52+ cells carried the low molecular weight variant (CD45RO+) of this molecule.Keywords juvenile rheumatoid arthritis yd T cells activation antigens CD69 leucocyte common antigen CD45RA CD45RO

γδT cells in Juvenile Idiopathic Arthritis: Higher Percentages of Synovial Vδ1+ and Vγ9+ T Cell Subsets Are Associated with Milder Disease

“…Previous studies addressed the issue of γδT cells in JIA 14,15,16,17 . Although healthy children display a gradual increase of Vγ9+ T cells with age (contrasting with our finding that in SF of JIA patients, the percentage of these cells decreased with age), no differences in the proportion of total γδT cells in the PB of patients and controls were found by some investigators 14,18 .…”

“…These authors also reported a lower expression of the joining region JγP (Jγ1.2), which is required for IPP reactivity, in the SF than in the PB of the patients. Kjeldsen-Kragh, et al found that both Vδ1+ and Vγ9+ cells in the SF displayed increased activation markers; the former were CD45RA+CD69+, whereas the latter expressed the CD45RO isoform, suggesting different levels of activation of the respective subsets 15 cells, none systematically analyzed these lymphocytes in different subtypes of JIA.…”

The Role of Gamma Delta T Cells in Autoimmune Rheumatic Diseases

“…Moreover, in another study of the SF of children with JRA, there were significantly more Vδ1 + CD69 + and Vδ2 + CD69 + cells compared with the PB of the same patients. The majority of the Vδ1 + cells expressed the high molecular weight isoform (CD45RA + ) while most of the Vδ2 + cells carried the low molecular weight variant (CD45RO + ) of this molecule [75]. In addition, when samples of PB (n = 25) and SF (n = 93) were analyzed by flow cytometry in 93 JIA patients, Vδ1 + and Vγ9 + γδ T cell subsets were detected in SF of all patients.…”