A high performance liquid chromatography method for simultaneous detection of 20 bioactive components in tea extracts

Abstract

A high performance liquid chromatography method for simultaneous detection of 20 bioactive components in tea extractsTea is the second most widely consumed beverage and contains various bioactive compounds. A simple method to analyze these compounds is of great scientific and commercial interest. In this work, a 30 min HPLC method was developed using a simple gradient elution system, and the mobile phases and elution gradients were optimized. This method separated 17 polyphenols and three alkaloid compounds in tea extracts, including catechins, alkaloids, phenolic acids, flavonols, and flavone, which are responsible for the bioactivity and flavor of tea. Excellent linearity was observed for all standard calibration curves, and correlation coefficients were above 0.9994. Heatmap analysis demonstrated significant separation between green, black, and pu-erh tea samples. The method described here is accurate and sensitive enough for the determination of active components in tea and could potentially be applied to other food products for the comprehensive investigation of their quality.

Region identification of Xinyang Maojian tea using UHPLC‐Q‐TOF/MS‐based metabolomics coupled with multivariate statistical analyses

“…The chemical compounds were separated with an Agilent C18 chromatographic column (250 × 4.6 mm, 5 µm) protected with a Phenomenex C18 guard column (10 mm × 4.6 mm, 5 µm; Phenomenex, Torrance, CA, USA) by reference to the chromatographic conditions described in the studies of Nian et al. (2019) and Guo et al. (2018).…”

“…An Agilent 1200 HPLC system (Agilent Technologies, Santa Clara, CA, USA) consisted of infinity binary pump, integrated vacuum degasser, autosampler, thermostated column compartment, and diode array detector (DAD) were used to determine the contents of caffeine, theobromine, GA, and the catechins, including C, EC, EGC, GC, ECG, GCG and EGCG, as well as the flavonoids including quercetin, kaempferol, myricetin and rutin. The chemical compounds were separated with an Agilent C18 chromatographic column (250 × 4.6 mm, 5 µm) protected with a Phenomenex C18 guard column (10 mm × 4.6 mm, 5 µm; Phenomenex, Torrance, CA, USA) by reference to the chromatographic conditions described in the studies of Nian et al (2019) and Guo et al (2018).…”

Unpruning improvement the quality of tea through increasing the levels of amino acids and reducing contents of flavonoids and caffeine

“…The concentrations of the 2 alkaloids and 16 polyphenols in tea leaves were determined using an Agilent 1200 series HPLC system having a Poroshell 120 EC-C18 column (4.6 × 100 mm, 2.7 μm) fitted with a C18 guard column (Agilent Technologies, Santa Clara, CA, USA). The detailed elution conditions have been described previously ( 19 ). The contents of 18 amino acids in tea leaves were measured in accordance with previous reports ( 20 , 21 ).…”

Phytochemical profiles of edible flowers of medicinal plants of Dendrobium officinale and Dendrobium devonianum

“…The extraction was performed in a water bath (at 85℃) for 90 min. The extractions were diluted to 50 ml and filtered through a 0.2‐μm nylon filter and then analyzed directly by HPLC as described in our previous work (Nian et al., 2019 ).…”