A high-performance liquid chromatographic technique for the determination of 2,5-piperazinedione in complex reaction mixtures

The mechanism of the reaction of the Nash and the Sawicki aldehyde reagent

“…The reaction sequence found to occur in the method of Sawicki and Carnes (2) has been partially described with regard to the identity of the intermediates (18,22) and the general reaction sequence (14,23,24), but not in the context of the original reaction by Vorlander (7)(8)(9) nor from an analytical point of view (i.e., with Sawicki's assay system in mind). The results of the study of reaction 10 indicate that compound 8 is formed immediately followed by the final product, 9.…”

“…The hplc studies were conducted using a system previously 0.923 g; mp 140-1420C; ir 3200-3600 (b), 1695 (s); nmr 6: 1.3(3), described (18) except that the normal phase mode was employed 2.2(3), 2.6(1), 3.6(2), all singlets; ms: 212,150,112,109,43 (base). with a p-Porasil column (Waters Associates, Milford, CT).…”