Because of its advantage of spectrum effectiveness and multi-path transmission, orthogonal frequency division multiplexing (OFDM) system gains extensive attention. OFDM technology combining with MIMO technology which can be faster and more reliable has become the key of fourth generation wireless communication. In order to support MIMO-OFDM system to conduct wireless real-time transmission of high-definition video, a channel estimation scheme is needed in receiver to overcome frequency selective fading. In this paper, the channel estimation scheme applies least square algorithm to estimate the channel parameter on reference signal position, and adopts twodimensional linear interpolation algorithm to estimate the channel parameter on user data position. The scheme is implemented and optimized by pipeline architecture of binary tree and Advanced Extensible Interface. According to test, this scheme occupies less FPGA resources, and is able to overcome channel fading in laboratory environment.