A high molecular weight form of terminal deoxynucleotidyl transferase

Bollum, F. J.; Brown, McKAY

doi:10.1038/278191a0

Cited by 45 publications

(16 citation statements)

References 9 publications

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“…Rearrangement is also characterized by the expression of rearrangement-specific genes. Such transcripts include RAG-1 and RAG-2 needed for DNA cleavage (7), TdT that facilitates the addition of nontemplated nucleotides (8), and various other DNA regulatory and repair enzymes. The presence of these rearrangement-specific products and transcripts can also be used for monitoring of B cell development.…”

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confidence: 99%

B Cell Lymphogenesis in Swine Is Located in the Bone Marrow

Šinkora

Šinkorová

2014

The Journal of Immunology

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A course and a site of B cell development in swine are not firmly known. In this study, we show that B cell lymphogenesis is located in the bone marrow (BM). According to expression of MHC class II (MHC-II), CD2, CD21, CD25, CD45RC, CD172a, swine workshop cluster (identification number) (SWC) 7, and μHC, porcine BM cells were resolved into seven subsets representing sequential stages of development. Profile of rearrangement-specific products and transcripts from sorted BM cells confirmed the proposed developmental pathway. The same developmental pathway was further proven by analysis of selection for productive rearrangements in Ig H chains and also by cultivation studies. Cultivation also showed that earliest precursors with incomplete DJ rearrangements can still revert their B cell differentiation and develop along myeloid lineage, whereas this is impossible for later developmental stages. Proliferation and the apoptotic potential of individual developmental stages as well as critical checkpoints were also identified. Colocalization experiments showed early colocalization of MHC-II/CD2/CD172a is replaced by colocalization of MHC-II/CD2/CD21/SWC7/IgM in immature cells, whereas CD25 and CD45RC did not colocalize with any other studied molecules. In this study, we also finally prove that the BM in pigs is fully functional in adult animals and that B lymphogenesis occurs there throughout life. To our knowledge, this is the first study showing a course and a direct site of B cell lymphogenesis in swine.

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confidence: 99%

B Cell Lymphogenesis in Swine Is Located in the Bone Marrow

Šinkora

Šinkorová

2014

The Journal of Immunology

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“…In this respect we should recall that TdT species with unexpectedly high mol. wt of about 60,000 have been noted in the neoplastic cells from two leukaemic patients (Diebel & Coleman, 1979) and from cultured human lymphoblastoid cells (Bollum & Brown, 1979). As a matter of fact, our value of 4-2 S for the Lm22 would correspond to an apparent molecular size close to that estimated for the larger TdT enzymes.…”

Section: Sedimentation Prqfiles Of Human Tdtmentioning

confidence: 70%

“…Bollum correlated the distinct molecular species of TdT with their intracellular location and function in thymus and nonthymic tissues (Bollum & Brown, 1979). He suggested that the large TdT polypeptide in the nucleus of prothymocytes might be the functionally active enzyme which undergoes a post-translational cleavage of its peptide chain during differentiation in the thymus.…”

Section: Discussionmentioning

confidence: 99%

“…wt (Bollum & Brown, 1979;Deibel & Coleman, 1979) were matched by the results of our sedimentation analyses of the active extracts from LL tissues. Like the analogous leukaemias, these lymphoblastic tumours might consist of cell clones with functional expression of one or other form of TdT.…”

Section: Discussionmentioning

confidence: 99%

“…Refined immunofluorescence methods showed that, in adult animals, TdT is localized to the nuclei of marrow cells and of large cortical thymocytes and to the cytoplasm of the small cortical thymocytes (Gregoire et al, 1979). Apparently, the high-mol.-wt human TdT isolated from lymphoblastoid cells in culture (Bollum & Brown, 1979) and from leukaemic cells (Deibel & Coleman, 1977) is devoid of subunit structure and consists of a single polypeptide.…”

Section: Discussionmentioning

confidence: 99%

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Terminal deoxynucleotidyl transferase in human lymphomas: possible existence of forms with high and low molecular weights

Vezzoni¹,

Campagnari²,

Fronzo³

et al. 1981

Br J Cancer

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Optimized methods for extraction and enzyme assay in crude tissue preparations were used to determine the amounts of terminal deoxnucleotidyl transferase (TdT) in malignant lymphomas. The TdT concentration was increased only in lymphoblastic lymphomas (LL) and was as high in these tumours as in the white blood cells from untreated patients with acute lymphoblastic leukaemia (ALL). The enzymes extracted from such lymphomas and from the leukaemic lymphoblasts had the same properties. Moreover, forms of TdT with low and high mol. wt were found in the LL tumours, similar to other reports of TdT-positive leukaemias. The overall study points at some basic biochemical identity of certain lymphoblastic malignancies, irrespective of whether the transformed cells are in solid tumours or are disseminated in the blood.

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