A high-mobility group box 1 that binds to DNA, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, Sebastes schlegelii

Zhao, Xin-peng; Hu, Yonghua; Liu, Yong; Wang, Jingjing; Wang, Guanghua; Wang, Renjie; Zhang, Min

doi:10.1016/j.fsi.2016.07.034

Cited by 34 publications

(12 citation statements)

References 39 publications

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“…The study of Zebrafish found that HMGB1 in brain may be closely related to brain development and repair after spinal cord injury. The higher expression of SpHMGB1 in the brain was also observed in studies of Turbot, Goldfish, Black Rockfish, and Nile Tilapia (El Gazzar 2007; Xie et al 2014; Zhao et al 2016; Cai et al 2018; Niu et al 2021). Furthermore, SpHMGB1 was highly expressed in peripheral immune tissues (spleen), mucosal immune tissues (intestine), and immune‐related tissues (hepatopancreas and kidney), suggesting that Sp HMGB1 may play a role in immune response.…”

Section: Discussionsupporting

confidence: 61%

“…Recombinant HMGB1 of Goldfish effectively increased the respiratory burst and released TNF‐α and IL‐1β in macrophages (Xie et al 2014). Recombinant HMGB1 significantly improved the antimicrobial activity of macrophages in Red Drum and Black Rockfish (Zhao et al 2011, 2016). The recombinant protein of Nile Tilapia HMGB1 not only promoted the expression of pro‐inflammatory related factors (e.g., IL‐6 and interleukin 8 [ IL‐8 ]) and macrophage migration inhibitory factor but also up‐regulated the anti‐inflammatory factor IL‐10 (Niu et al 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Among bony fish, the HMGB1 gene was first cloned in red Crucian Carp Carassius auratus red var. and Blunt Snout Bream Megalobrama amblycephala (Liu et al 2009) and then was also successfully identified in the Zebrafish Danio rerio (Zhao et al 2011), Goldfish Carassius auratus (Xie et al 2014), Grass Carp Ctenopharyngodon idella (Yang et al 2013), Black Rockfish Sebastes schlegelii (Zhao et al 2016), Humphead Snapper Lutjanus sanguineus (Cai et al 2014), Red Drum Sciaenops ocellatus (Zhao et al 2011), Turbot Scophthalmus maximus (Cai et al 2018), Rohu Labeo rohita (Lenka et al 2018), Arctic Lamprey Lampetra japonica (Pang et al 2012; Li et al 2019), Nile Tilapia Oreochromis niloticus (Niu et al 2021), and Yellow Catfish Pelteobagrus fulvidraco (Wang et al 2021). Structurally, HMGB1 has two DNA‐binding domains that are positively charged, termed the A box and B box, and a highly acidic C‐terminal region that is negatively charged (Wang et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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Expression profiling and inflammatory activation analysis of high‐mobility group box 1 in Schizothorax prenanti

Chen

Hong

et al. 2022

J Aqua Anim Hlth

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Objective High‐mobility group box 1 (HMGB1) is a highly conserved nuclear protein and participates in the immune response to pathogens in bony fish. In this study, the structure and function of HMGB1 in the cyprinid fish Schizothorax prenanti (SpHMGB1) were investigated. Methods The spatial structure of SpHMGB1 was predicted by CPHmodels. Quantitative reverse transcription PCR was used to detect the mRNA of SpHMGB1 in different tissues and Streptococcus agalactiae infection. The macrophage was treated with synthetic SpHMGB1‐B box peptide to analyze the inflammatory activity. Result Structurally, SpHMGB1 had the conserved A box, B box, and acid tail compared with Zebrafish Danio rerio and mice Mus musculus. SpHMGB1 was universally expressed in various tissues, with the highest expression in the middle kidney. In vivo, SpHMGB1 was significantly induced in response to Streptococcus agalactiae infection in the blood and spleen. Synthetic SpHMGB1‐B box peptide activated respiratory burst and up‐regulated the messenger RNA expression of interleukin‐1β, tumor necrosis factor α, interleukin‐10, interferon regulatory factor 1, interferon regulatory factor 7, C‐X‐C motif chemokine ligand 11‐1, C‐X‐C motif chemokine ligand 11‐2, and toll‐like receptor 4 in macrophages. Conclusion This study suggested that SpHMGB1 participated in the response to bacterial pathogens and that SpHMGB1‐B box peptide played an important role in mediating the immune response of S. prenanti.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression profiling and inflammatory activation analysis of high‐mobility group box 1 in Schizothorax prenanti

Chen

Hong

et al. 2022

J Aqua Anim Hlth

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“…These complexes act synergistically to aggravate pancreatitis via cognate receptors to the HMGB1-partner molecules. Although the list of reported HMGB1 receptors is fairly extensive, only two receptor systems, MOK protein kinase and Toll-like receptor (TLR)4, have been fully confirmed to act as genuine HMGB1 receptors (30). TLR4-deficient mice have been shown to succumb to endotoxemia in the presence of increased levels of HMGB1, while caspase11-deficient mice survive (31).…”

Section: Mir-340-5p Inhibits Pancreatic Acinar Cell Inflammation and Apoptosis Via Targeted Inhibition Of Hmgb1mentioning

confidence: 99%

miR‑340‑5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1

et al. 2021

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Acute pancreatitis (AP) is a common gastrointestinal disease that affects 1 million individuals worldwide. Inflammation and apoptosis are considered to be important pathogenic mechanisms of AP, and high mobility group box 1 (HMGB1) has been shown to play a particularly important role in the etiology of this disease. MicroRNAs (miRs) are emerging as critical regulators of gene expression and, as such, they represent a promising area of therapeutic target identification and development for a variety of diseases, including AP. Using the online database query (microRNA.org), the current study identified a site in the 3' untranslated region of HMGB1 mRNA that was a viable target for miR-340-5p. The present study aimed to investigate the association between miR-340-5p and HMGB1 expression in pancreatic acinar cells following lipopolysaccharide (LPS) treatment by performing luciferase, western blotting and reverse transcription-quantitative PCR assays. The results suggest that miR-340-5p attenuates the induction of HMGB1 by LPS, thereby inhibiting inflammation and apoptosis via blunted activation of Toll-like receptor 4 and enhanced AKT signaling. Thus, the therapeutic application of miR-340-5p may be a useful strategy in AP via upregulation of HMGB1 and subsequent promotion of inflammation and apoptosis.

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“…Therefore, studies on black rockfish immune-related genes can expand our understanding of ncRNA that related to immune response and regulatory mechanisms, and are also helpful to guide the prevention and control of its diseases. Previous studies have investigated several immune-related genes, such as high mobility group box 1, c-type lectin, galectin-8, cathepsin K, chemokine ligand 25, and melanocortin-4, in black rockfish in response to pathogen stimulation (32)(33)(34)(35)(36)(37). However, no systematic report on the interactions of ncRNAs and coding RNAs in S. schlegelii during infection has been performed.…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of circRNA-miRNA-mRNA Regulatory Networks in the Intestine of Sebastes schlegelii Following Edwardsiella tarda Challenge

Cao

et al. 2021

Front. Immunol.

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Sebastes schlegelii, an important aquaculture species, has been widely cultured in East Asian countries. With the increase in the cultivation scale, various diseases have become major threats to the industry. Evidence has shown that non-coding RNAs (ncRNAs) have remarkable functions in the interactions between pathogens and their hosts. However, little is known about the mechanisms of circular RNAs (circRNAs) and coding RNAs in the process of preventing pathogen infection in the intestine in teleosts. In this study, we aimed to uncover the global landscape of mRNAs, circRNAs, and microRNAs (miRNAs) in response to Edwardsiella tarda infection at different time points (0, 2, 6, 12, and 24 h) and to construct regulatory networks for exploring the immune regulatory mechanism in the intestine of S. schlegelii. In total, 1,794 mRNAs, 87 circRNAs, and 79 miRNAs were differentially expressed. The differentially expressed RNAs were quantitatively validated using qRT-PCR. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that most of the differentially expressed mRNA genes and the target genes of ncRNAs were related to immune signaling pathways, such as the NF-κB signal pathway, pathogen recognition receptors related to signaling pathways (Toll-like receptors and Nod-like receptors), and the chemokine signaling pathway. Based on these differentially expressed genes, 624 circRNA-miRNA pairs and 2,694 miRNA-mRNA pairs were predicted using the miRanda software. Integrated analyses generated 25 circRNA-miRNA-mRNA interaction networks. In a novel_circ_0004195/novel-530/IκB interaction network, novel_530 was upregulated, while its two targets, novel_circ_0004195 and IκB, were downregulated after E. tarda infection. In addition, two circRNA-miRNA-mRNA networks related to apoptosis (novel_circ_0003210/novel_152/apoptosis-stimulating of p53 protein 1) and interleukin (novel_circ_0001907/novel_127/interleukin-1 receptor type 2) were also identified in our study. We thus speculated that the downstream NF-κB signaling pathway, p53 signaling pathway, and apoptosis pathway might play vital roles in the immune response in the intestine of S. schlegelii. This study revealed a landscape of RNAs in the intestine of S. schlegelii during E. tarda infection and provided clues for further study on the immune mechanisms and signaling networks based on the circRNA-miRNA-mRNA axis in S. schlegelii.

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A high-mobility group box 1 that binds to DNA, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, Sebastes schlegelii

Cited by 34 publications

References 39 publications

Expression profiling and inflammatory activation analysis of high‐mobility group box 1 in Schizothorax prenanti

Expression profiling and inflammatory activation analysis of high‐mobility group box 1 in Schizothorax prenanti

miR‑340‑5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1

Integrated Analysis of circRNA-miRNA-mRNA Regulatory Networks in the Intestine of Sebastes schlegelii Following Edwardsiella tarda Challenge

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