A high efficacy DNA vaccine against Tilapia lake virus in Nile tilapia (Oreochromis niloticus)

Yu, Nana; Zeng, Weiwei; Zhou, Xiong; Liu, Zhixin

doi:10.1016/j.aqrep.2022.101166

Cited by 13 publications

(12 citation statements)

References 29 publications

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“…As previously observed [ 29 , 74 ], vaccine efficacy is ultimately influenced by the dose of the vaccine being administered and the use of adjuvants. This is also exemplified in a study by Alberer et al [ 129 ] in which the efficacy of vaccination against rabies virus with an mRNA vaccine was found to be highly dependent on the dose and the route of administration.…”

Section: Factors That Can Influence Vaccine Efficacymentioning

confidence: 90%

“…Another DNA vaccine (pcDNA3.1–ORF10) against TiLV infection was developed and tested by Yu et al [ 74 ] for its efficacy against TiLV in Nile tilapia. After IM vaccination with this DNA vaccine, the authors observed a significant upregulation of immune-related genes encoding for IgM, TLR2, MyD88, IL8, TNFα, IFN-γ, and NF-κB in the spleen, liver, and kidney of pcDNA3.1–ORF10-vaccinated tilapia, especially those encoding for IFN-γ in the liver, IgM, IFN-γ, and NF-κB in the spleen, and IL-8 and TNF-α in the kidneys.…”

Section: Types Of Fish Vaccines and Current Vaccine Approaches For Th...mentioning

confidence: 99%

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Tilapia Lake Virus Vaccine Development: A Review on the Recent Advances

et al. 2023

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Tilapia tilapinevirus (or tilapia lake virus, TiLV) is a recently emerging virus associated with a novel disease affecting and decimating tilapia populations around the world. Since its initial identification, TiLV has been reported in 17 countries, often causing mortalities as high as 90% in the affected populations. To date, no therapeutics or commercial vaccines exist for TiLV disease control. Tilapia exposed to TiLV can develop protective immunity, suggesting that vaccination is achievable. Given the important role of vaccination in fish farming, several vaccine strategies are currently being explored and put forward against TiLV but, a comprehensive overview on the efficacy of these platforms is lacking. We here present these approaches in relation with previously developed fish vaccines and discuss their efficacy, vaccine administration routes, and the various factors that can impact vaccine efficacy. The overall recent advances in TiLV vaccine development show different but promising levels of protection. The field is however hampered by the lack of knowledge of the biology of TiLV, notably the function of its genes. Further research and the incorporation of several approaches including prime–boost vaccine regimens, codon optimization, or reverse vaccinology would be beneficial to increase the effectiveness of vaccines targeting TiLV and are further discussed in this review.

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Section: Factors That Can Influence Vaccine Efficacymentioning

confidence: 90%

Section: Types Of Fish Vaccines and Current Vaccine Approaches For Th...mentioning

confidence: 99%

Tilapia Lake Virus Vaccine Development: A Review on the Recent Advances

et al. 2023

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“…Following a viral infection, fish respond by producing an early antiviral response elicited by the innate response. [48][49][50] An antibody response 51,52 and cytotoxic T-cell immunity 53 are subsequently produced by the adaptive immune response.…”

Section: Current Understanding Of the Fish's Immune Response To Tilvmentioning

confidence: 99%

“…65 The entire ORF of TiLV Segment 10 was incorporated into a DNA vaccine (the eukaryotic expression vector pcDNA3.1) and subsequently tested in a TiLV vaccine trial in Nile tilapia. 53 Fish receiving an IM injection of pcDNA3.1-ORF10…”

Section: The Current Status Of Tilv Vaccines For Tilapiamentioning

confidence: 99%

“…showed increased survival compared to fish that received an IM injection with the pcDNA3.1 expression vector alone, with RPS as high as 85.7% in fish vaccinated with 450 μg/mL of pcDNA3.1-ORF10. 53 This improved survival correlated with very low viral loads in the kidney, liver and spleen and elevated immune gene expression in each of these tissues, including igm, tlr2, mdf88, il-8, tnfα, ifn-γ and nf-κB. 53 However, a key consideration is that gene expression of immunerelated genes does not necessarily correlate with improved protection against disease.…”

Section: The Current Status Of Tilv Vaccines For Tilapiamentioning

confidence: 99%

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Tilapia lake virus disease: Vaccine strategies to control the threat to tilapia aquaculture

Lakshmi¹,

Smith

Dong

et al. 2023

Reviews in Aquaculture

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Since the first report of a novel disease caused by tilapia lake virus (TiLV) in 2014, the disease (TiLVD) has gained considerable attention as one of the most devastating viral diseases in tilapia aquaculture globally. The virus is both highly virulent and transmissible and has been detected in many countries worldwide. It poses a severe threat to the global tilapia aquaculture industry, food security and many people's livelihoods in low and middle‐income countries. The absence of effective treatment strategies and inadequate biosecurity measures means the virus's rapid spread is likely to continue. Vaccination has proven to be an effective approach to preventing and controlling disease in finfish aquaculture. The vaccine's efficacy and the delivery system's suitability are essential for evoking an appropriate immune response against the pathogen. The purpose of this review is to provide an overview of the strategies being used to develop vaccines for TiLVD. Nanovaccines, which offer a new approach to prevent TiLVD in farmed tilapia, are also discussed.

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Alternative therapies recently applied in controlling farmed fish diseases: mechanisms, challenges, and prospects

Elgendy,

Ali,

Dayem

et al. 2024

Aquacult Int

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The aquaculture industry is suffering from significant financial setbacks due to an increasing frequency of disease outbreaks, posing a threat to the sector’s sustainability. Various bacterial, viral, parasitic, and fungal pathogens have led to massive mortalities in farmed fish worldwide. Throughout the years, the management of fish diseases has predominantly centered around the utilization of conventional antibiotics and chemicals. Nevertheless, their indiscriminate use has given rise to serious implications, including an increase in resistant pathogens, disruptions in the metabolic processes of fish, degradation of the aquatic environment, the presence of drug residues in aquatic products, and a potential threat to human health. Various effective bio-based and immunoprophylaxis alternative therapies have been developed to overcome these impediments. Recent alternative therapeutic approaches to fish diseases encompass a range of strategies, including phytotherapeutics, nanotherapeutics, probiotics, prebiotics, synbiotics, phage therapy, vaccination, quorum quenching, antimicrobial peptides, biosurfactants, bacteriocins, stem cells, and diagnostic-based therapy. Advancements in biotechnology have significantly enhanced the efficacy of these therapies. However, additional research is essential to refine the utilization of these therapeutic approaches. Critical concerns, such as efficacy, cost, risks, availability, and adverse effects on fish and the ecosystem, need to be addressed to establish guidelines for their sustainable application in aquaculture. This review will increase aquaculturists’ awareness of recent therapies used in fish farming, their mechanisms, challenges, and impacts while promoting the sustainability of commercial aquaculture.

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A high efficacy DNA vaccine against Tilapia lake virus in Nile tilapia (Oreochromis niloticus)

Cited by 13 publications

References 29 publications

Tilapia Lake Virus Vaccine Development: A Review on the Recent Advances

Tilapia Lake Virus Vaccine Development: A Review on the Recent Advances

Tilapia lake virus disease: Vaccine strategies to control the threat to tilapia aquaculture

Alternative therapies recently applied in controlling farmed fish diseases: mechanisms, challenges, and prospects

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