A high-density linkage map of the RN region in pigs

Abstract

The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906 . The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene.

Association of Porcine IGF Binding Protein-5 Gene with Meat Quality

“…The IGFBP-5 gene has been mapped on SSC15, and multipoint linkage analysis has revealed the order FN1-IGFBP-5-S1000-S1001-LR8RB-VIL1-RN-Sw936-Sw906 (Looft et al 2000). Together with the results of Rohrer et al (1997), we found that porcine IGFBP-5 is located between 78 and 85 cm (Wang 2006).…”

“…The IGFBP-5 gene was mapped on porcine chromosome 15 (Looft et al 2000). The mRNA for this gene is 966 bp, and the deduced amino acid sequence consists of 252 amino acids and a putative 19 amino acid signal (White et al 1996).…”

Chromosome Location and Association of Haplotypes of Insulin-like Growth Factor Binding Protein-2 with Production Performance in Swine

“…To date, porcine IGFBP-1 (Inage-Miyake et al 2005), -3 (Lahbib et al 1996), -4 (Wang et al 2005, and -5 (Looft et al 2000) have been mapped on the porcine chromosome but not IGFBP-2. Also, no studies have been done on the association between the polymorphism and the production performance of this gene.…”