A high-density genetic map developed by specific-locus amplified fragment (SLAF) sequencing and identification of a locus controlling anthocyanin pigmentation in stalk of Zicaitai (Brassica rapa L. ssp. chinensis var. purpurea)

Li, Guihua; Chen, Hancai; Liu, Jiali; Luo, Wenlong; Xie, Dasen; Luo, Shuhong; Wu, Tianbin; Akram, Waheed; Zhong, Yixi

doi:10.1186/s12864-019-5693-2

Cited by 28 publications

(29 citation statements)

References 66 publications

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“…chinensis var. purpurea), BrbHLH49, BrEGL3.2 and BrMYBL2.1 [19][20][21] ; purple bok choy (B. rapa L. ssp. chinensis), BrMYB73 22 ; purple tumorous stem mustard (Brassica juncea var.…”

Section: Introductionmentioning

confidence: 99%

The novel gene BrMYB2, located on chromosome A07, with a short intron 1 controls the purple-head trait of Chinese cabbage (Brassica rapa L.)

Xue

et al. 2020

Hortic Res

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Anthocyanins are important secondary metabolites in plants, but information on anthocyanin biosynthesis mechanisms in Chinese cabbage is limited. The new purple head Chinese cabbage cultivar 11S91 was analyzed, and an R2R3-MYB regulatory gene BrMYB2, located on chromosome A07, controlling the dominant purple-head trait was isolated. High expression of BrMYB2 generated a large accumulation of anthocyanins in 11S91, accompanied by highly upregulated BrTT8, BrF3′H, BrDFR1, BrANS1, BrUGTs, BrATs, and BrGSTs. 11S91 inherited the purple locus from purple trait donor 95T2-5, and they shared consensus CDSs and gDNAs with those of BrMYB2 (cBrMYB2 and gBrMYB2). Two SNPs in cBrMYB2 in 11S91 did not cause loss of function; in addition to several SNPs at both ends of intron 1, a large deletion had occurred in intron 1 of gBrMYB2 in 11S91. Genetic transformation of Arabidopsis showed that gBrMYB2 overexpression lines presented deeper purple color and higher expression than did the cBrMYB2 and cBrmyb2 lines, whereas gBrmyb2 with a long intron 1 did not cause the purple phenotype. We first show that BrMYB2 promotes anthocyanin biosynthesis under the control of the short intron 1 of gBrMYB2 in purple head Chinese cabbage, and gBrmyb2 with a long intron 1 represses anthocyanin production in white head Chinese cabbage. This evidence provides a new understanding of anthocyanin biosynthesis and purple germplasm generation in Brassica vegetables.

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“…chinensis var. purpurea), BrbHLH49, BrEGL3.2 and BrMYBL2.1 [19][20][21] ; purple bok choy (B. rapa L. ssp. chinensis), BrMYB73 22 ; purple tumorous stem mustard (Brassica juncea var.…”

Section: Introductionmentioning

confidence: 99%

The novel gene BrMYB2, located on chromosome A07, with a short intron 1 controls the purple-head trait of Chinese cabbage (Brassica rapa L.)

Xue

et al. 2020

Hortic Res

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“…BrMYB73 was predicted to encode a R2R3-MYB transcription factor, and one deletion and one SNP were found in this gene in purple-leaf parent. Recently, Li et al identified a bHLH49 transcription factor (BrbHLH49) on A07 that might positively regulate the anthocyanin accumulation in Zicaitai based on a specific-locus amplified fragment sequencing method 19 . These studies revealed the genetic diversity of anthocyanin biosynthesis related genes in Brassica crops.…”

Section: Discussionmentioning

confidence: 99%

“…The purple pigment of Zicaitai has been confirmed as anthocyanins 4 . Although several studies have characterized anthocyanins in Brassica crops [16][17][18][19] , there is limited information on the genes involved in anthocyanin biosynthesis in Zicaitai.…”

mentioning

confidence: 99%

QTL-Seq and Sequence Assembly Rapidly Mapped the Gene BrMYBL2.1 for the Purple Trait in Brassica rapa

Zhang

et al. 2020

Sci Rep

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Anthocyanins have strong antioxidant activity and are believed to be healthy for human beings. the Brassica rapa L. ssp. chinensis var. purpurea "Zicaitai" is rich in anthocyanins. We constructed an f 2 population of Zicaitai and "caixin" (Brassica rapa ssp. parachinensis) and it shows clear segregation of the purple phenotype (i.e., variation in anthocyanin enrichment). Here, quantitative trait locus (QTL)-Seq was performed with two sample groups from the F 2 population: one exhibiting an intense purple phenotype and the other showed a completely green phenotype. The results showed that the QTL-Seq and linkage analysis located different major loci. This indicates that there are two major genetic factors that plays different roles in regulating anthocyanin enrichment in Zicaitai. This was further supported by the data simulation of an in silico f 2 population that QTL-Seq and linkage analysis can locate different major loci. Furthermore, the draft genomes of the two parents (Zicaitai and Caixin) were assembled and utilized to search for mutations in candidate genes. A ~100-bp insertion was found in the third exon of gene BrMYBL2.1 in Zicaitai. BrMYBL2.1 is a negative regulator of anthocyanin biosynthesis, while BrEGL3.2-previously located by linkage mapping-is a positive regulator. for these populations with multiple genes contributing large effects to a trait, a strategy of low depth re-sequencing of F 2 individuals followed by QTL-Seq analysis with the free combination of sample groups is proposed. Furthermore, draft-sequence assembly of parental genomes together with QTL mapping is suggested as an efficient means for fine-mapping genes rapidly in segregating populations.Anthocyanin pigments are important flavonoid compounds that exhibit a wide range of biological functions in plants 1-3 , including as attractants for pollinators and seed dispersers and in protecting plants against abiotic and biotic stresses 4,5 . More importantly, anthocyanins show beneficial effects to human health and exhibit potential protective functions against cancer and heart disease 6,7 . These properties are partially attributed to their strong antioxidant capacity 8 . According to studies in the model plant Arabidopsis thaliana, regulatory anthocyanin genes can be mainly divided into positive and negative regulatory genes based on whether they promote or inhibit the expression of structural genes for anthocyanin biosynthesis, respectively 9 . Positive regulatory factors primarily include three types of genes: R2R3-MYB 10 , basic helix-loop-helix (bHLH), and WD40 transcription factors 11 . These genes promote the biosynthesis of anthocyanins. Additionally, there are two main types of negative regulatory genes, including the R3-MYB transcription factor 10,12 and the nitrogen-induced LBD transcription factor 13 . The bioavailability and activity of anthocyanins and their regulatory genes vary widely across plants 5,14 . "Zicaitai" (Brassica rapa L. ssp. chinensis var. utilis), a representative purple variety of B. rapa, exhibits purple...

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“…Благодаря высокому содержанию уникальных биологически активных соединений в съедобных стеблях и листьях пурпурная капуста пользуется спросом в странах Азии, Европы и Америки (Li et al, 2019). В последние годы эта культура стала востребованным объектом для молекулярных и генетических исследований.…”

Section: Introductionunclassified

“…Такие исследования начаты и в отношении культуры B. purpuraria (Guo et al, 2015). Ведутся работы по созданию генетической карты для идентификации локусов контролирующих накопление антоциана в стебле растения (Li et al, 2019).…”

Section: Introductionunclassified

Production of doubled haplois in Brassica purpuraria

et al. 2019

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Relevance. In recent years vegetable crop Brassica rapa ssp. chinensis var. purpuraria (synonyms: Brassica campestris L. var.purpurea Bailey; Brassica rapa L. ssp. chinensis var. purpurea) is gaining popularity as an object of genetic and molecular researches, and as an economically valuable vegetable plant due to the high content of biologically active compounds and distinctive economically valuable traits. Effective technology for development DH-plants to accelerate the breeding process for this culture has not been developed yet, so research in this area is relevant.Materials and methods. The study included two varieties from the collection of Vavilov AllRussian Research Institute of Plant Industry (VIR): No. 1301 (China) and No. 1357(Netherlands). Both protocols standard unmodified and with addition of silver nitrate (AgNO3) in the medium for embryogenesis induction were used in experiments for production of DH plants from isolated microspore in vitro. Direct chromosome counting in meristem cells and flow cytometry were used to determine the ploidy of regenerating plants.Results. As a result of the study embryogenesis in B. purpuraria culture can develop with the use of a standard protocol as well as with the addition of silver nitrate that showed a positive effect on the induction of embryogenesis. The yield of embryoids varied depending on the genotype of the individual plant within the variety accession. The highest yield of embryoids was 40 embryoids/petri dish. The main problem at the stage of regeneration is that about half of the regenerating plants occurred to be albinos and were not viable. We show a high degree of spontaneous chromosome doubling in regenerated plants (all analyzed plants were doubled haploids). In total 38 regenerated plants were obtained from accession No. 1301. It was shown that four DH-plants had self-incompatibility after self-pollination, but seed progeny from other plants was obtained. The created material was taken for genetics study and breeding work.

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A high-density genetic map developed by specific-locus amplified fragment (SLAF) sequencing and identification of a locus controlling anthocyanin pigmentation in stalk of Zicaitai (Brassica rapa L. ssp. chinensis var. purpurea)

Cited by 28 publications

References 66 publications

The novel gene BrMYB2, located on chromosome A07, with a short intron 1 controls the purple-head trait of Chinese cabbage (Brassica rapa L.)

The novel gene BrMYB2, located on chromosome A07, with a short intron 1 controls the purple-head trait of Chinese cabbage (Brassica rapa L.)

QTL-Seq and Sequence Assembly Rapidly Mapped the Gene BrMYBL2.1 for the Purple Trait in Brassica rapa

Production of doubled haplois in Brassica purpuraria

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