A High‐Affinity Fluorescent Sensor for Catecholamine: Application to Monitoring Norepinephrine Exocytosis

Zhang, Le; Liu, Xin A.; Gillis, Kevin D.; Glass, Timothy E.

doi:10.1002/anie.201810919

Cited by 105 publications

(53 citation statements)

References 30 publications

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“…Since their discovery, fluorescent probes have been engineered to accurately monitor and quantify in real-time an increasing variety of physiological parameters in living cells such as ion concentration variations [1] , cysteines [2] , pH [3] , enzymatic activity [4] or oxidative stress [5] , [6] , [7] , [8] , but also various cellular compounds [9] , [10] , [11] , [12] . Genetically-encoded fluorescent proteins are of particular interest as they can be addressed to specific organelles, such as the nucleus or the mitochondria, allowing a subcellular resolution [13 , 14] .…”

Section: Methods Detailsmentioning

confidence: 99%

PiQSARS: A pipeline for quantitative and statistical analyses of ratiometric fluorescent biosensors

et al. 2020

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Genetically encoded ratiometric fluorescent probes are cutting-edge tools in biology. They allow precise and dynamic measurement of various physiological parameters within cell compartments. Because data extraction and analysis are time consuming and may lead to inconsistencies between results, we describe here a standardized pipeline for Semi-automated treatment of time-lapse fluorescence microscopy images. Quantification of individual cell signal. Statistical analysis of the data. First, a dedicated macro was developed using the FIJI software to reproducibly quantify the fluorescence ratio as a function of time. Raw data are then exported and analyzed using R and MATLAB softwares. Calculation and statistical analysis of selected graphic parameters are performed. In addition, a functional principal component analysis allows summarizing the dataset. Finally, a principal component analysis is performed to check consistency and final analysis is presented as a visual diagram. The method is adapted to any ratiometric fluorescent probe. As an example, the analysis of the cytoplasmic HyPer probe in response to an acute cell treatment with increasing amounts of hydrogen peroxide is shown. In conclusion, the pipeline allows to save time and analyze a larger amount of samples while reducing manual interventions and consequently increasing the robustness of the analysis.

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Section: Methods Detailsmentioning

confidence: 99%

PiQSARS: A pipeline for quantitative and statistical analyses of ratiometric fluorescent biosensors

et al. 2020

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“…Organic p-conjugated fluorescent materials have been investigated in the past several decades owing to their promising applications in organic light-emitting diodes (OLEDs), [1][2][3][4][5][6] chemical sensors [7][8][9][10][11] and biological imaging. [12][13][14][15][16][17] However, the notorious problem of these classical luminescent materials is the familiar aggregation-caused quenching (ACQ), i.e., they weakly emit in their aggregated states such as powder and crystals compared to their dilute solutions.…”

Section: Introductionmentioning

confidence: 99%

Each phenyl group performs its own functions on luminescence: phenyl substituted effect in tetraphenylpyrazine

Song

Zhang

et al. 2020

Mater. Chem. Front.

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“…Fluorescent materials have an important role in modern society because of their extensive applications in chemical and biological sensors, anti-counterfeit, forensic and biomedical imaging, arts and entertainment industries, etc. [1][2][3][4] However, most of traditional fluorophores suffer from aggregation-caused quenching (ACQ) effect, 5 which has seriously restricted their applications in concentrated solutions and in solid state. In contrast, aggregation induced emission (AIE) effect has received great attention since the pioneering work by Tang's group in 2001.…”

Section: Introductionmentioning

confidence: 99%

An Intrinsic Hydroquinone-Functionalized AIE Core with Dual Sensitivity via Integrated Molecular Design

Wang

et al. 2020

Preprint

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A new aggregation-induced emission (AIE) luminescence core, 1-hydroquinol-1,2,2-triphenylethene (HQTPE), has been designed and synthesized for the first time. This AIE core is amazingly simple but is fundamentally important to chemistry because of its intrinsic redox and pH activities. The incorporation of hydroquinone (HQ) moiety into a common AIE core tetraphenylethene (TPE) yields HQTPE with unique fluorescent properties over most other AIEgens so far reported. There are tremendous differences of photochemcical properties between HQTPE, 1-benzoquinol-1,2,2-triphenylethene (QTPE, the oxidized counterpart) and its anions. Interestingly, as the solution concentration is increased, AIEgen HQTPE shows stronger fluorescence but QTPE exhibits rapid quenching of fluorescence in a nonlinear fashion, which are in agreement with theoretical studies. The fluorescence of HQTPE is also highly dependent of pH value of media. We have further explored HQTPE as an ultrasensitive redox probe and efficient deoxidizer, which could lead to many potential applications in health care, food security, environmental monitoring, optic and electronic devices.

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A High‐Affinity Fluorescent Sensor for Catecholamine: Application to Monitoring Norepinephrine Exocytosis

Cited by 105 publications

References 30 publications

PiQSARS: A pipeline for quantitative and statistical analyses of ratiometric fluorescent biosensors

PiQSARS: A pipeline for quantitative and statistical analyses of ratiometric fluorescent biosensors

Each phenyl group performs its own functions on luminescence: phenyl substituted effect in tetraphenylpyrazine

An Intrinsic Hydroquinone-Functionalized AIE Core with Dual Sensitivity via Integrated Molecular Design

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