A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

Abstract

The Chinese mitten crab (Eriocheir sinensis) is a popular aquaculture product in East Asia, especially in China. In the last decade, rice–crab co-culture has rapidly expanded in China. Under this model, crabs are raised in rice fields instead of in traditional aquaculture ponds. In this study, we cultured two varieties of Chinese mitten crabs (Changjiang and Liaohe) in an alkaline region in northwest China and used Illumina MiSeq sequencing to compare the intestinal bacterial alpha diversity and community structure between traditional and co-culture aquaculture models, between two crab varieties, and between female and male crabs. Significant variations in intestinal bacterial communities were found between crab varieties and between female and male crabs but not between aquaculture models. These results show that rice–crab co-culture operations did not obviously impact the crab intestinal bacterial community compared with traditional pond aquaculture. Firmicutes was the most abundant bacterial phylum in the crab intestines (78%, relative abundance). Three dominant operational taxonomic units (OTUs) represented 73.2% of Firmicutes sequences and 56.8% of all sequences. A dominant OTU assigned as Firmicutes that was negatively correlated with crab body length, width, and weight was found in the source water for the experimental area. The results of this study suggest that the aquaculture of Chinese mitten crabs in alkaline regions requires more study to improve cultivation techniques.

Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors

“…It can be influenced by several factors, including genetic background, sex, developmental stage, habitat environment, feed type, health conditions, and external stimuli ( Piazzon et al, 2020 ). While nongenetic factors have been extensively studied for their impact on the changes in the intestinal microorganisms of E. sinensis ( Chen et al, 2021a , b ; Guan et al, 2021 ; Guo et al, 2021 ; Jiang et al, 2022 ; Shao et al, 2022 ), little research has been conducted on the effects of host genetic background on the composition of the intestinal microflora in this economically significant species. This study investigated the gut microbiome communities and metabolic profiles in juveniles of two E. sinensis crab varieties with different shell colors.…”

Effects of different temperatures on growth and intestinal microbial composition of juvenile Eriocheir sinensis

“… Chen et al (2015 ) studied the microflora in the intestines of E. sinensis cultured in Tai Lake and found that Proteobacteria, Bacteroidetes, and Tenericutes were the dominant groups. Guan et al (2021 ) found that the dominant flora in the intestinal microflora of E. sinensis in an alkaline region in Northwest China was Firmicutes. In addition, there is relatively little research on the effect of temperature on the relationship between gut microbiota and growth of E. sinensis .…”

Exploration of the roles of microbiota on biogenic amines formation during traditional fermentation of Scomber japonicus

“…Since spoilage potentials and metabolic characteristics of different microbes vary significantly and could be affected by microbial interactions ( Lu et al, 2018 ). High-throughput sequencing (HTS) with the properties of high flux and short experimental cycle has recently been applied to the analysis of microbial systems in different food matrices, such as fish sauce ( Wang et al, 2018 ), reef fishes ( Gao et al, 2020 ), and Pacific white shrimp ( Guan et al, 2021 ). A great potential of HTS is facilitating the exploration of the relationships between the microbiota composition and specific variables like BAs ( Hao and Sun, 2020 ), salinity ( Mengjuan et al, 2021 ).…”