A hexasaccharide from capsular polysaccharide of carbapenem-resistant Klebsiella pneumoniae KN2 is a ligand of Toll-like receptor 4

Lee, I‐Ming; Huang, Tzu-Yin; Fu, Yang; Johansson, Victor; Hsu, Chun-Ru; Hsieh, Pei-Fang; Chen, Shin‐Tai; Yang, Yen-Ju; Konradsson, Peter; Sheu, Jyh‐Horng; Wang, Jann-Tay; Wu, Shih‐Hsiung

doi:10.1016/j.carbpol.2021.118944

Cited by 2 publications

(2 citation statements)

References 46 publications

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“…As such, the O -acetyl group in TFP-F1 is essential for immunomodulatory activity. The relationship between the structures and immunomodulatory activities of TFP-F1 derivatives was in agreement with our previous research [ 26 , 27 , 41 ].…”

Section: Resultssupporting

confidence: 92%

An Immunological Polysaccharide from Tremella fuciformis: Essential Role of Acetylation in Immunomodulation

Huang

Chiu

et al. 2022

IJMS

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The edible fungus Tremella fuciformis was shown to have a high molecular weight (1.87 × 103 kDa) bioactive polysaccharide, denoted as TFP-F1. Monosaccharide composition and NMR analysis of the polysaccharide and its derivatives indicated it contained fucose (Fucp), xylose (Xylp), mannose (Manp), and glucuronic acid (GlcAp) in a ratio of 0.9:1.0:3.2:1.2. Using IR, NMR, and GC-MS spectroscopic data, the structure of TFP-F1 was elucidated as {→3)-[β-D-GlcAp-(1→2)]-α-D-Manp-(1→3)-α-D-Manp-(1→3)-[α-L-Fucp-(1→2)-β-D-Xylp-(1→2)]-α-D-Manp-(1→}n, with partial acetylation of C6-OH in mannoses. Furthermore, at a concentration of 1 μg/mL, TFP-F1 was found to stimulate the secretion of TNF-α and IL-6 in J774A.1 macrophage cells in vitro via interaction with toll-like receptor 4 (TLR4). The removal of O-acetyl groups led to the loss of immunomodulatory activities, demonstrating that O-acetyl groups play an essential role in enhancing the production of pro-inflammatory cytokines.

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Section: Resultssupporting

confidence: 92%

An Immunological Polysaccharide from Tremella fuciformis: Essential Role of Acetylation in Immunomodulation

Huang

Chiu

et al. 2022

IJMS

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“…The latest experiments showed that capsular polysaccharide (CPS) derived from carbapenem-resistant K. pneumoniae KN2 serotype can stimulate J774A.1 mouse macrophage to release TNF-α and IL-6 in vitro . The CPS also exerts an immune response through TLR4 in human embryonic kidney-293 (HEK-293) cells ( Lee et al., 2022 ). Melissa and Kovach observed that the clearance rate of K. pneumoniae in IL-36γ-deficient mice was decreased and the mortality of the mouse was increased, which confirmed that IL-36γ is related to the anti- Klebsiella effect.…”

Section: Innate Immune Responses To K Pneumoniaementioning

confidence: 99%

Host defense against the infection of Klebsiella pneumoniae: New strategy to kill the bacterium in the era of antibiotics?

Liang

Wang²,

Lai³

et al. 2022

Front. Cell. Infect. Microbiol.

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Klebsiella pneumoniae (K. pneumoniae) is a typical gram-negative iatrogenic bacterium that often causes bacteremia, pneumonia and urinary tract infection particularly among those with low immunity. Although antibiotics is the cornerstone of anti-infections, the clinical efficacy of β-lactamase and carbapenems drugs has been weakened due to the emergence of drug-resistant K. pneumoniae. Recent studies have demonstrated that host defense plays a critical role in killing K. pneumoniae. Here, we summarize our current understanding of host immunity mechanisms against K. pneumoniae, including mechanical barrier, innate immune cells, cellular immunity and humoral immunity, providing a theoretical basis and the new strategy for the clinical treatment of K. pneumoniae through improving host immunity.

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A hexasaccharide from capsular polysaccharide of carbapenem-resistant Klebsiella pneumoniae KN2 is a ligand of Toll-like receptor 4

Cited by 2 publications

References 46 publications

An Immunological Polysaccharide from Tremella fuciformis: Essential Role of Acetylation in Immunomodulation

An Immunological Polysaccharide from Tremella fuciformis: Essential Role of Acetylation in Immunomodulation

Host defense against the infection of Klebsiella pneumoniae: New strategy to kill the bacterium in the era of antibiotics?

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