A hexameric peptide purified from Styela plicata protects against free radical-induced oxidative stress in cells and zebrafish model

Ko, Seok‐Chun; Kim, Eun-A; Jung, Won‐Kyo; Kim, Won‐Suk; Lee, Seung‐Cheol; Son, Kwang Tae; Kim, Jae-Il; Jeon, You‐Jin

doi:10.1039/c6ra06308j

Cited by 9 publications

(27 citation statements)

References 44 publications

(42 reference statements)

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“…35 According to the previous reports, bioactive peptides have widely been utilized to improve their functional and nutritional properties via enzymatic hydrolysis method. Bioactive peptides can act as potential metabolism regulator that it reported various bioactivities such as osteoblast differentiation, 11 anti-oxidant, 36 glucose uptake, 37 and anti-allergic 38 activities. In particular, Jung et al had reported that fish bone peptide could not only promote bone formation and mineralization but also induce higher calcium solubility owing to increased calcium absorption.…”

Section: Discussionmentioning

confidence: 99%

Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor

Heo

Nam

et al. 2018

Cell Biochemistry & Function

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Fish bone, a by-product of fishery processing, is composed of protein, calcium, and other minerals. The objective of this study was to investigate the effects of a bioactive peptide isolated from the bone of the marine fish, Johnius belengerii, on the osteoblastic differentiation of MC3T3-E1 pre-osteoblasts. Post consecutive purification by liquid chromatography, a potent osteogenic peptide, composed of 3 amino acids, Lys-Ser-Ala (KSA, MW: 304.17 Da), was identified. The purified peptide promoted cell proliferation, alkaline phosphatase activity, mineral deposition, and expression levels of phenotypic markers of osteoblastic differentiation in MC3T3-E1 pre-osteoblast. The purified peptide induced phosphorylation of mitogen-activated protein kinases, including p38 mitogen-activated protein kinase, extracellular regulated kinase, and c-Jun N-terminal kinase as well as Smads. As attested by molecular modelling study, the purified peptide interacted with the core interface residues in bone morphogenetic protein receptors with high affinity. Thus, the purified peptide could serve as a potential pharmacological substance for controlling bone metabolism.

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Section: Discussionmentioning

confidence: 99%

Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor

Heo

Nam

et al. 2018

Cell Biochemistry & Function

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“…Notably, some studies omitted the UF step and directly purified protein hydrolysates on an SEC and/or IEC column [45,46,56,57]. Sephadex G-15, G-25 and G-50 are SEC stationary phases that have been used in the purification of antioxidant peptides from marine invertebrate hydrolysates.…”

Section: Enzyme-assisted Production Purification and Identificatmentioning

confidence: 99%

“…The use of both SEC and IEC to purify antioxidant peptides from the protein hydrolysates of the prawn [48], Indian squid [57], shortclub cuttlefish [56], sea squirt [45], and shrimp processing by-products [54] has also been reported. When purifying antioxidant peptides from the sea cucumber, Zhou, Wang and Jiang [55] adopted the approach of two-step SEC linked by IEC.…”

Section: Enzyme-assisted Production Purification and Identificatmentioning

confidence: 99%

“…Cell types used in these studies were human lung fibroblast cells [41,43], Chang liver cells [32], RAW 264.7 [45], and rat cerebral microvascular endothelial cells (RCMEC) [42]. Notably, the cytoprotective activity of PIIVYWK, FSVVPSPK, VKP, and VKCFR was confirmed using chemically synthesized peptide sequences, rather than purified fractions [32,42].…”

Section: Evaluation Of Antioxidant Activitiesmentioning

confidence: 99%

“…The effectiveness of the marine invertebrate peptides in scavenging ROS, chelating metals, suppressing lipid peroxidation, and protecting cells against ROS-induced toxicity has been demonstrated by using chemical and cell-based assays [ 40 , 41 , 42 , 43 ]. Notably, the in vivo effects of antioxidant peptides identified from the mussel [ 44 ] and sea squirt [ 45 ] were reported, suggesting that antioxidant peptides identified from edible marine invertebrates can have biological or physiological significance. Collectively, the aforementioned findings suggest that edible marine invertebrates deserve more attention than they have received to date as a promising source of potent antioxidant peptides.…”

Section: Introductionmentioning

confidence: 99%

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Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

et al. 2017

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Marine invertebrates, such as oysters, mussels, clams, scallop, jellyfishes, squids, prawns, sea cucumbers and sea squirts, are consumed as foods. These edible marine invertebrates are sources of potent bioactive peptides. The last two decades have seen a surge of interest in the discovery of antioxidant peptides from edible marine invertebrates. Enzymatic hydrolysis is an efficient strategy commonly used for releasing antioxidant peptides from food proteins. A growing number of antioxidant peptide sequences have been identified from the enzymatic hydrolysates of edible marine invertebrates. Antioxidant peptides have potential applications in food, pharmaceuticals and cosmetics. In this review, we first give a brief overview of the current state of progress of antioxidant peptide research, with special attention to marine antioxidant peptides. We then focus on 22 investigations which identified 32 antioxidant peptides from enzymatic hydrolysates of edible marine invertebrates. Strategies adopted by various research groups in the purification and identification of the antioxidant peptides will be summarized. Structural characteristic of the peptide sequences in relation to their antioxidant activities will be reviewed. Potential applications of the peptide sequences and future research prospects will also be discussed.

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Improving antioxidant and antiproliferative activities of colla corii asini hydrolysates using ginkgo biloba extracts

Guo²,

Hao³

et al. 2018

Food Science & Nutrition

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Colla corii asini hydrolysates (ACCH) and ginkgo biloba extracts (EGb) possess more potent antioxidant effects when used in combination than when used alone. The mixture of ACCH and EGb at a dose ratio of 20:4(w:w) showed the highest radical scavenging activity with IC 50 of 0.17 ± 0.01, 0.43 ± 0.02 and 1.52 ± 0.07 mg/ml against DPPH, ABTS and HO · free radicals, respectively. Furthermore, the inhibition of breast cancer cells MCF‐7 and MDA‐MB‐231 proliferation increased when these cell lines were treated with a combination of ACCH and EGb for 72 hr, with IC 50 of 4.32 ± 0.12 mg/ml and 0.39 ± 0.01 mg/ml, respectively. The findings indicated that the mixtures of ACCH and EGb could be used to prevent and treat some diseases caused by the excessive free radicals, especially cancer. Therefore, the mixtures of ACCH and EGb might serve as a natural source of desirable antioxidant and anticancer agents for the nutraceutical and pharmaceutical industries.

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A hexameric peptide purified from Styela plicata protects against free radical-induced oxidative stress in cells and zebrafish model

Abstract: To prepare antioxidative peptide from Styela plicata, nine proteases were employed for enzymatic hydrolysis, and the antioxidative activities of the hydrolysates were investigated using an electron spin resonance (ESR) spectrometer.

Cited by 9 publications

References 44 publications

Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor

Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor

Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

Improving antioxidant and antiproliferative activities of colla corii asini hydrolysates using ginkgo biloba extracts

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