A Heterologous Radioimmunoassay Method for Measurement of Hamster Growth Hormone

Borer, Katarina T.; Kelch, Robert P.

doi:10.1159/000122730

Cited by 10 publications

(8 citation statements)

References 13 publications

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“…Un responsiveness to prolactin secretory stimuli ( fig. 6) esta-blished that hypophysectomy effectively abolished stimu lated prolactin release as it abolished the release of GH [5].…”

Section: Discussionmentioning

confidence: 93%

Hamster Prolactin: Physiological Changes in Blood and Pituitary Concentrations as Measured by a Homologous Radioimmunoassay

Borer¹,

Kelch²,

Corley³

1982

Neuroendocrinology

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A specific and sensitive homologous radioimmunoassay (RIA) for determination of hamster prolactin is described and compared to heterologous hamster prolactin RIA and to the homologous and heterologous RIAs for rat prolactin. With this method, we have determined that, in the golden hamster, diurnal plasma prolactin fluctuations have a mean interpeak interval of 1.5 h, that serum prolactin concentrations are influenced by day length, by ether vapors, by animal’s gender, and by refeeding following a fast but not by neurosurgical procedures which increase somatic growth and serum GH concentration in adult hamsters. Variations in day length influenced pituitary prolactin content and concentration, and pituitary removal abolished prolactin secretory responses to ether stress. A heterologous RIA for hamster prolactin which utilizes radioiodinated rat prolactin and rabbit antihamster prolactin serum (RK1–15) represents the most advantageous method for the measurement of hamster prolactin because of its high sensitivity (0.14 ng/ml), high antiserum binding to iodinated rat prolactin, and the ability to measure both hamster and rat prolactin.

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“…Un responsiveness to prolactin secretory stimuli ( fig. 6) esta-blished that hypophysectomy effectively abolished stimu lated prolactin release as it abolished the release of GH [5].…”

Section: Discussionmentioning

confidence: 93%

Hamster Prolactin: Physiological Changes in Blood and Pituitary Concentrations as Measured by a Homologous Radioimmunoassay

Borer¹,

Kelch²,

Corley³

1982

Neuroendocrinology

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“…We have again [6] shown that ether stress with or without cardiac puncture elicits GH secretion in the hamster ( fig. 4.…”

Section: Discussionmentioning

confidence: 55%

“…SH AP was prepared from 4 adult female APs with a combined weight of 13.73 mg. Pituitaries were homogenized as described previously, and SHAP was used at 9 serial solutions between 1.72 pgand 6.71 ng/tube. SHAP reference preparation was used to facil itate comparisons with the GH values obtained in the heterologous hamster GH assay with SHAP reference preparation [6].…”

Section: Methodsmentioning

confidence: 99%

“…each assay included eight serial dilutions in duplicate of hamster GH (AFP 1595-B) between 12.8 and 0.1 ng/tube. This GH preparation has a biological activity of greater than I lU/mg as de termined by the observed weight gain of 3 hypophysectomized rats treated with 50 pg SC for 5 days [6], Each assay also included three serial dilutions in triplicate of a normal hamster standard serum pool. All RIA data collected in Ann Arbor including parallelism determinations, were performed by the computer program des cribed by Duddleson et al [18],…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…11, 13], Studies of the accompanying changes in serum and pituitary growth hormone (GH) concentration [5] have been hampered by the unavailability of a direct method for measurement of hamster GH. Serial dilutions of hamster sera and pituitary extracts [36] or of purified hamster GH [6] suppress the binding between rat GH and antiserum against rat GH in a nonparallel fashion. To alleviate this problem, we have developed a specific and sensitive radioimmunoassay (RIA) for measurement of hamster GH using purified gol den hamster GH (AFP 1595-B) for iodination and reference preparation and an antiserum developed against this hor mone in a rhesus monkey [8].…”

mentioning

confidence: 99%

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Hamster Growth Hormone

1982

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A specific and sensitive homologous radioimmunoassay (RIA) for determination of golden hamster growth hormone (GH) is described and compared to a heterologous hamster GH RIA. Using the homologous system, cross-reactivity experiments between golden hamster GH on one hand, and pituitary extracts from two other hamster species, and purified GHs from several rodent and mammalian species, suggested that hamster GH differs from other rodent and mammalian GHs to a greater extent than rat GH. Using the homologous method, we have determined that, in the golden hamster, diurnal plasma fluctuations have a mean interpeak interval of 70- 80 min, that serum GH concentrations are affected by nyctohemeral differences in lighting, by ether vapors, by animal’s gender, and by starvation and refeeding. Basal GH concentrations were not influenced by exposure to different photoperiods or by removal of the gonads. A heterologous hamster GH RIA, which utilizes a radioiodinated rat GH and monkey antihamster GH serum, represents the most advantageous method for the measurement of hamster GH because of its high sensitivity (0.3 ng/ml), low limit of detection (46 pg/ml), high antiserum binding to iodinated rat GH, and the ability to measure rat and mouse GHs in addition to hamster GH. Immunological differences between hamster, rodent, and other mammalian GHs are diminished when heterologous, rather than homologous, tracer is used.

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