A Heterologous Radioimmunoassay for Follicle-Stimulating Hormone: Application to Measurement of FSH in the Ovine Estrous Cycle and in Several Other Species Including Man1

Salamonsen, Lois A.; Jonas, Helen A.; Burger, Henry; Buckmaster, Joan M.; Chamley, W. A.; Cumming, I. A.; Findlay, Jock K.; Goding, J. R.

doi:10.1210/endo-93-3-610

Cited by 77 publications

(46 citation statements)

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“…In addition, when the hormone profiles were standardized about the preovulatory surge, the pattern of FSH secretion monitored in the control ewes (see Fig. 3b) was comparable to that previously reported for GnRH-treated anoestrous ewes (McLeod & Haresign, 1987), and indeed, to that observed for naturally-cycling ewes during the breeding season (Salamonsen et al, 1973;Pant et al, 1977). For the first 30 h of treatment mean plasma LH concen¬ trations, and the LH response to GnRH injections, did not differ between treatment groups.…”

Section: Discussionsupporting

confidence: 84%

Suppression of plasma FSH concentrations with bovine follicular fluid blocks ovulation in GnRH-treated seasonally anoestrous ewes

McLeod

McNeilly²

1987

Reproduction

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Summary. The specific requirement for FSH in the final stages of preovulatory follicle development was assessed in seasonally anoestrous ewes given 2-h injections of GnRH (250 ng/injection), with (N = 10) or without (N = 10) concurrent treatment with bovine follicular fluid (bFF: 2 ml given i.v. at 8-h intervals). Treatment with bFF significantly (P < 0\m=.\01)suppressed plasma FSH concentrations, but, at least for the first 30 h of treatment, did not influence the magnitude of GnRH-induced LH episodes (mean max. conc. 3\m=.\00 \ m=+-\ 0\m=.\39and 3\m=.\63 \ m=+-\ 0\m=.\51 ng/ml for bFF-treated and control ewes, respectively). Of 10 animals treated with GnRH for 72 h, 5/5 control ewes showed oestrus and ovulated whereas 0/5 bFF-treated ewes showed oestrus or ovulated in response to GnRH treatment. There was, however, a transient (13\m=.\2\m=+-\ 1\m=.\0h) increase in plasma LH concentrations in the ewes given bFF (mean max. conc. 4\m=.\64\m=+-\1\m=.\57 ng/ml), which was coincident with the preovulatory LH surge recorded in animals given GnRH alone. In 10 GnRH-treated ewes slaughtered after 32 h of treatment, the mean diameter of the largest antral follicle was significantly (P < 0\m=.\001) greater in control ewes (5\m=.\92 \m=+-\0\m=.\17 mm) than in animals that were also given bFF (3\m=.\94\ m=+-\ 0\m=.\14 mm). In addition, the incidence of atresia in the 3 largest antral follicles present at this time was greater in bFF-treated ewes. These results show that, when plasma FSH concentrations are suppressed by administration of bFF, although the magnitude of GnRH-induced LH episodes is unchanged, preovulatory follicular development is impaired and ovulation does not occur. This may be indicative of a specific requirement for FSH in the final stages of preovulatory follicle development, or due to direct inhibitory effects of bovine follicular fluid.

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Section: Discussionsupporting

confidence: 84%

Suppression of plasma FSH concentrations with bovine follicular fluid blocks ovulation in GnRH-treated seasonally anoestrous ewes

McLeod

McNeilly²

1987

Reproduction

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“…Plasma FSH was measured using a heterologous double antibody radioimmunoassay with an antiserum (Butt M94) raised in a rabbit against human FSH (McNeilly et al 1976) and NIH-FSH-S6 (biopotency 1·24 x NIH-FSH-Sl) as standard and PapkoffFSH (G4-15OC; biopotency 54 x NIH-FSH-Sl) for preparation of tracer (Salamonsen et al 1973). This assay has been previously described by Bremner et al (1980).…”

Section: Hormone Assaysmentioning

confidence: 99%

Plasma LH and FSH in Ewes That Were Either Fertile or Infertile After Long-Term Grazing of Oestrogenic Pasture

Rodgers

Clarke

Findlay

et al. 1980

Aust. Jnl. Of Bio. Sci.

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The levels of plasma LH and FSH were measured in serial blood samples taken at 15-min intervals for 6 h from ewes that had remained fertile after grazing oestrogenic pasture (clover-fertile ewes), from ewes that were permanently affected by clover disease (clover-infertile ewes) and from normal ewes. Two flocks of ewes from different locations were studied. In flock I, tonic LH secretion (total area under the curve of LH concentration versus time, 1 area unit = 1 ng ml-1 x 1 h) was significantly (P < 0'05) greater in clover-infertile ewes (10'4 area units) during anoestrus than in ewes that had remained fertile after prolonged grazing of oestrogenic clover (5·4 area units). Tonic LH and FSH secretions during the breeding season and FSH secretion during anoestrus were not significantly different. In flock 2, LH levels during the breeding season were significantly (P < 0'05) elevated in clover-infertile ewes (10' 9 area units) compared to normal ewes (5' 4 area units) that had never grazed oestrogenic clover. LH secretion in clover-infertile ewes (7. 8 area units) was intermediate to that found in infertile and control ewes. Concentrations of FSH, progesterone and ovarian vein oestradiol-17 P (Ez) during the breeding season were similar in the three groups.In another experiment, the positive feedback release of LH following administration of Ez (12'5,25 or 50 f.1g per ewe) was measured in anoestrous ewes of flock 2. Significantly (P < 0·01) more clover-infertile ewes demonstrated a positive feedback effect than control ewes when given 12· 5 f.1g Ez but not when given higher doses.The elevation of LH secretion in permanently affected clover-infertile ewes is consistent with the hypothesis that the hypothalamo-pituitary axis of these ewes is less responsive to the negative feedback effect of oestrogen. Furthermore, the patency of the positive feedback loop is consistent with the ability to ovulate.Other keywords: Clover disease. IntroductionLong-term grazing of oestrogenic clover pastures may cause permanent infertility in ewes (Bennetts et al. 1946;Barrett et al. 1965) although not all ewes grazing oestrogenic clover exhibit infertility. Infertile ewes have uterine endometrial hyperplasia and changes in cell type and function of the cervix (Adams 1976). In addition, there may be an associated neuroendocrine lesion. For example, Findlay et al. (1973) have reported that when infertile ewes which had previously grazed oestrogenic clover were ovariectomized, they were less likely to show elevated plasma LH levels, i.e. positive feedback (Goding et al. 1969), in response to oestradiol-17f1 (E 2 ) treatment than were normal ovariectomized ewes. This reduced responsiveness of the hypothalamo-pituitary axis to E2 could be related to changes in the number or affinity

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“…Radioimmunoassay s FSH, LH and progesterone were measured in plasma by specific radioimmunoassays described previously (Scaramuzzi, Caldwell & Moor, 1970;Salamonsen et al, 1973; McNatty, Gibb, Dobson, Thurley & Findlay, 1981).…”

Section: Introductionmentioning

confidence: 99%

Use of bovine follicular fluid to increase ovulation rate or prevent ovulation in sheep

Henderson¹,

Prisk²,

Hudson³

et al. 1986

Reproduction

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A Heterologous Radioimmunoassay for Follicle-Stimulating Hormone: Application to Measurement of FSH in the Ovine Estrous Cycle and in Several Other Species Including Man1

Cited by 77 publications

References 1 publication

Suppression of plasma FSH concentrations with bovine follicular fluid blocks ovulation in GnRH-treated seasonally anoestrous ewes

Suppression of plasma FSH concentrations with bovine follicular fluid blocks ovulation in GnRH-treated seasonally anoestrous ewes

Plasma LH and FSH in Ewes That Were Either Fertile or Infertile After Long-Term Grazing of Oestrogenic Pasture

Use of bovine follicular fluid to increase ovulation rate or prevent ovulation in sheep

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