A HET-CAM based vascularized intestine tumor model as a screening platform for nano-formulated photosensitizers

Elberskirch, Linda; Harzic, Ronan Le; Scheglmann, Dietrich; Wieland, Gerhard D.; Wiehe, Arno; Mathieu-Gaedke, Maria; Golf, Hartwig R. A.; Briesen, Hagen von; Wagner, Sylvia

doi:10.1016/j.ejps.2021.106046

Cited by 5 publications

(3 citation statements)

References 49 publications

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“…A major advantage relies in the CAM’s easy accessibility and low rejection rate to facilitate increasing the model’s complexity by engrafting 3D spheroids ( 118 , 119 ) or multicellular organoids. For instance, mature organoids derived from human-induced pluripotent stem cells (PSCs) rapidly connected to the vascular network of the chick embryo after transferring them on the CAM ( 120 ), and PSC–derived inner ear and kidney organoids demonstrated the model’s potential to optimize developmental maturity and functionality of organoids based on vascularization ( 121 , 122 ).…”

Section: Discussionmentioning

confidence: 99%

In ovo model in cancer research and tumor immunology

2022

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Considering cancer not only as malignant cells on their own but as a complex disease in which tumor cells interact and communicate with their microenvironment has motivated the establishment of clinically relevant 3D models in past years. Technological advances gave rise to novel bioengineered models, improved organoid systems, and microfabrication approaches, increasing scientific importance in preclinical research. Notwithstanding, mammalian in vivo models remain closest to mimic the patient’s situation but are limited by cost, time, and ethical constraints. Herein, the in ovo model bridges the gap as an advanced model for basic and translational cancer research without the need for ethical approval. With the avian embryo being a naturally immunodeficient host, tumor cells and primary tissues can be engrafted on the vascularized chorioallantoic membrane (CAM) with high efficiencies regardless of species-specific restrictions. The extraembryonic membranes are connected to the embryo through a continuous circulatory system, readily accessible for manipulation or longitudinal monitoring of tumor growth, metastasis, angiogenesis, and matrix remodeling. However, its applicability in immunoncological research is largely underexplored. Dual engrafting of malignant and immune cells could provide a platform to study tumor-immune cell interactions in a complex, heterogenic and dynamic microenvironment with high reproducibility. With some caveats to keep in mind, versatile methods for in and ex ovo monitoring of cellular and molecular dynamics already established in ovo are applicable alike. In this view, the present review aims to emphasize and discuss opportunities and limitations of the chicken embryo model for pre-clinical research in cancer and cancer immunology.

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Section: Discussionmentioning

confidence: 99%

In ovo model in cancer research and tumor immunology

2022

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“…Cell testing in the murine C6 glioma cell line revealed apoptosis as the main cell death mechanism. Elberskirch et al (226) used the HET-CAM model with the duodenum adenocarcinoma cell line HuTu-80 employing temoporfin-loaded PLGA nanoparticles as well as nanoparticles with two porphyrin-based photosensitizers, finding that the model was suitable to simulate in vivo endoscopic irradiation. PLGA nanoparticles coated with a phospholipid bilayer have also been tested for co-encapsulation of temoporfin and a chemotherapeutic, exhibiting an additive effect against a retinoblastoma cell line (183).…”

Section: Plga Nanoparticlesmentioning

confidence: 99%

“…Visudyne®, which contains the photosensitizer verteporfin as the active substance and is used in the treatment of the age‐related macular degeneration, is such a freeze‐dried liposomal formulation to be re‐constituted before administration to the patient (387). Various liposomal formulations of temoporfin have been prepared and related photosensitizers have been incorporated into liposomes as well (226), e.g. the temoporfin analogue temocene (78).…”

Section: Pharmacology and Biochemistrymentioning

confidence: 99%

The Photosensitizer Temoporfin (mTHPC) – Chemical, Pre‐clinical and Clinical Developments in the Last Decade^†^‡

Wiehe

Senge

2022

Photochem & Photobiology

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This review follows the research, development and clinical applications of the photosensitizer 5,10,15,20-tetra(mhydroxyphenyl)chlorin (mTHPC, temoporfin) in photodynamic (cancer) therapy (PDT) and other medical applications. Temoporfin is the active substance in the medicinal product Foscanâ authorized in the EU for the palliative treatment of head and neck cancer. Chemistry, biochemistry and pharmacology, as well as clinical and other applications of temoporfin are addressed, including the extensive work that has been done on formulation development including liposomal formulations. The literature has been covered from 2009 to early 2022, thereby connecting it to the previous extensive review on this photosensitizer published in this journal [Senge, M. O. and J. C. Brandt (2011) Photochem. Photobiol. 87, 1240-1296] which followed its way from initial development to approval and clinical application.

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Exploring Current In Vitro Models for Cancer Research

Gilazieva,

Kitaeva,

Issa

et al. 2024

Interdisciplinary Cancer Research

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A HET-CAM based vascularized intestine tumor model as a screening platform for nano-formulated photosensitizers

Cited by 5 publications

References 49 publications

In ovo model in cancer research and tumor immunology

In ovo model in cancer research and tumor immunology

The Photosensitizer Temoporfin (mTHPC) – Chemical, Pre‐clinical and Clinical Developments in the Last Decade^†^‡

Exploring Current In Vitro Models for Cancer Research

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A HET-CAM based vascularized intestine tumor model as a screening platform for nano-formulated photosensitizers

Cited by 5 publications

References 49 publications

In ovo model in cancer research and tumor immunology

In ovo model in cancer research and tumor immunology

The Photosensitizer Temoporfin (mTHPC) – Chemical, Pre‐clinical and Clinical Developments in the Last Decade†‡

Exploring Current In Vitro Models for Cancer Research

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Product

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The Photosensitizer Temoporfin (mTHPC) – Chemical, Pre‐clinical and Clinical Developments in the Last Decade^†^‡