A Herpetological Survey of the James Bay Area of Québec and Ontario

Desroches, Jean-François; Schueler, Frederick W.; Picard, Isabelle; Gagnon, Langis

doi:10.22621/cfn.v124i4.1101

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…Today, Western North American populations, including those in Alberta, British Columbia, Colorado, and Nevada have dramatically declined [7-10]. Northeastern Ontario populations of L. pipiens have also been declining over the last 40 years [11]. …”

Section: Introductionmentioning

confidence: 99%

Efficient induction of spawning of Northern leopard frogs (Lithobates pipiens) during and outside the natural breeding season

Trudeau

Schueler

Navarro‐Martín

et al. 2013

Reprod Biol Endocrinol

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BackgroundAmphibian declines are now recognized globally. It is also well known that many anurans do not reproduce easily in captivity, especially when held over long periods, or if they require hibernation before breeding. A simple method to induce spawning and subsequent development of large numbers of healthy tadpoles is therefore required to meet research and conservation goals.MethodsThe method is based on simultaneous injection of both female and male leopard frogs, Lithobates pipiens (formerly called Rana pipiens) with a cocktail of a gonadotropin-releasing hormone agonist (GnRH-A) and a dopamine antagonist. We call this the AMPHIPLEX method, which is derived from the combination of the words amphibian and amplexus. Following injection, the animals are thereby induced, and perform amplexus and natural fertilization under captive conditions.ResultsWe tested combinations of a GnRH agonist with 2 different dopamine antagonists in L. pipiens in the breeding season. The combination of des-Gly10, D-Ala6, Pro-NHEt9-GnRH (0.4 micrograms/g body weight; GnRH-A) with metoclopramide hydrochloride (10 micrograms/g body weight; MET) or domperidone (DOM) were equally effective, producing 89% and 88% successful spawning, respectively. This yielded more than 44,000 eggs for the 16/18 females that ovulated in the GnRH-A+MET group, and more than 39,000 eggs for the 15/17 females that ovulated in the GnRH-A+DOM group. We further tested the GnRH-A+MET in frogs collected in the wild in late autumn and hibernated for a short period under laboratory conditions, and report a low spawning success (43%). However, GnRH-A priming 24 hours prior to injections of the GnRH-A+MET cocktail in animals hibernated for 5–6 weeks produced out-of-season spawning (89%) and fertilization (85%) comparable to those we observed for in-season spawning. Assessment of age and weight at metamorphosis indicated that L. pipiens tadpoles resulting from out-of-season spawning grew normally and metamorphosed successfully.ConclusionWe provide evidence for successful captive breeding of the leopard frog, L. pipiens. This simple protocol can be used to obtain large numbers of eggs in a predictable, timed manner.

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Section: Introductionmentioning

confidence: 99%

Efficient induction of spawning of Northern leopard frogs (Lithobates pipiens) during and outside the natural breeding season

Trudeau

Schueler

Navarro‐Martín

et al. 2013

Reprod Biol Endocrinol

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“…These range margin declines are clearly an ongoing large scale phenomenon in the northeastern U.S. and Canada since the 1990s (Schueler, 1999). However, at broad geographical scales chorus frog ranges may be shifting, rather than simply retracting, because of possible P. maculata range expansions in northern Quebec (Ouellet et al, 2009;Desroches et al, 2010). If this finding is not simply due to increased survey efforts, the species will have recently crossed a major river system that was previously believed to be a barrier (Schueler, 1973).…”

Section: Resultsmentioning

confidence: 99%

“…Boreal chorus frogs may have undergone a sizeable range expansion on the far northern range margin at James Bay in Ontario and Quebec where the frogs inhabit open bogs (Ouellet et al, 2009) and brackish pools (Desroches et al, 2010). Unfortunately the paucity of historical surveys at this remote location makes it difficult to determine whether this finding is the result of a recent range expansion or earlier post-glacial reinvasion.…”

Section: Boreal Chorus Frogmentioning

confidence: 99%

Shifting status and distribution of range margin chorus frog (Pseudacris) populations in eastern Great Lakes watersheds

Corser¹,

Roblee²,

Johnson³

2012

Journal of Great Lakes Research

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“…To my knowledge, this is the first reported case of communal oviposition of E. bislineata in Ontario. The range of the Northern Two-lined Salamander is surprisingly extensive in Ontario (Desroches et al 2010) and other eastern Canadian provinces (Cook 1984), but few researchers visit these regions. Continued observation of this species in the northernmost part of its range would be of value.…”

Section: Discussionmentioning

confidence: 99%

Communal Oviposition in the Northern Two-lined Salamander (<em>Eurycea bislineata</em>) in Algonquin Provincial Park, Ontario

LeGros

2012

Can Field Nat

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While surveying a stream for amphibians in Algonquin Provincial Park, Ontario, Canada, I discovered a clutch of Two-lined Salamander (Eurycea bislineata) eggs on the underside of a partially submerged rock. I counted 165 eggs and measured them using ImageJ from a digital photograph. The quantity of eggs is far greater than any known clutch size for this species, and it was likely deposited by more than one female. This is the first report of communal oviposition in this species in Canada.

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A Herpetological Survey of the James Bay Area of Québec and Ontario

Cited by 8 publications

References 11 publications

Efficient induction of spawning of Northern leopard frogs (Lithobates pipiens) during and outside the natural breeding season

Efficient induction of spawning of Northern leopard frogs (Lithobates pipiens) during and outside the natural breeding season

Shifting status and distribution of range margin chorus frog (Pseudacris) populations in eastern Great Lakes watersheds

Communal Oviposition in the Northern Two-lined Salamander (<em>Eurycea bislineata</em>) in Algonquin Provincial Park, Ontario

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