Long-term immunosuppressed renal transplant recipients with chronic hepatitis B virus (HBV) infection often develop liver cirrhosis (LC) and end-stage liver disease (ESLD).This study investigated accumulation and persistence of specific HBV mutants in relation to the clinical course in these patients (n ؍ 38; mean follow-up, 3.5 years). HBV was analyzed longitudinally via length polymorphism of polymerase chain reaction (PCR) fragments (median, 6.5 serum samples per patient) as well as by cloning and partial sequencing of 346 full-length HBV genomes. Fourteen patients (group 1) developed LC or died from ESLD, whereas 24 patients (group 2) showed no evidence of LC during follow-up. Development of LC and ESLD was associated with persistence of HBV mutant populations characterized by deletions/insertions in core promoter plus deletions in the C gene and/or deletions in the pre-S region (86% of group 1 vs. 17% of group 2; P < .0001). HBV without these mutations or with core promoter mutations alone were predominantly found in group 2 (14% of group 1 vs. 75% of group 2). In patients infected with core promoter mutants, the additional appearance and persistence of deletions in the C gene and/or the pre-S region were accompanied or followed by development of LC and ESLD. The mutations were distributed on individual genomes in various combinations, leading to a high complexity of the virus population. In conclusion, these data suggest that accumulation and persistence of specific HBV populations characterized by mutations in 3 subgenomic regions play a role in pathogenesis of LC and ESLD in long-term renal transplant recipients. (HEPATOLOGY 2002;35:
466-477.)H epatitis B virus (HBV) infection is associated with various courses of liver disease ranging from asymptomatic carrier state to fulminant hepatitis. It is generally accepted that liver injury is immune mediated. However, severe liver disease and liver cirrhosis (LC) are also observed in long-term immunosuppressed patients such as renal transplant recipients with chronic HBV infection, 1-4 which is not fully compatible with immunopathogenesis. In past years, there was increasing evidence that HBV mutants may be involved in hepatopathogenesis in immunosuppressed patients. Mutations in the core promoter/enhancer II (Cp/EnII) that create a novel hepatocyte nuclear factor (HNF) 1 binding site and deletions in the pre-S region were found in a heart transplant recipient who died from fulminant HBV infection under immunosuppression. 5 A liver transplant recipient died from fibrosing cholestatic hepatitis following the accumulation of HBV with deletions in the pre-S1/2 region. 6 In a previous study, we showed that HBV with deletions in the C gene is significantly associated with development of HBV-related end-stage liver disease (ESLD) and death in renal transplant recipients. 7 Whereas the former studies reported just single cases without a control group, the latter was focused on 1 subgenomic region of HBV. Therefore, on the HBV genome level, it is still unclear wh...