A heparin-functionalized carbon nanotube-based affinity biosensor for dengue virus

Wasik, Daniel; Mulchandani, Ashok; Yates, Marylynn V.

doi:10.1016/j.bios.2017.01.017

Cited by 84 publications

(61 citation statements)

References 47 publications

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“…The immunosensor calibration plot for NS1 in PB was linear between 1.0 ng/mL and 1000.0 ng/mL (y = −4.88x − 35.23, R 2 = 0.99; n = 7) with a sensitivity (slope) of −4.88 ± 0.13% per log ng/mL. The increase in resistance to NS1 additions is likely due to the electron scattering/donation potential onto semiconducting p -type SWNT [ 33 , 36 , 37 ].…”

Section: Resultsmentioning

confidence: 99%

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Wasik

Mulchandani

Yates

2018

Sensors

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Dengue virus (DENV) is a highly pathogenic, arthropod-borne virus transmitted between people by Aedes mosquitoes. Despite efforts to prevent global spread, the potential for DENV epidemics is increasing world-wide. Annually, 3.6 billion people are at risk of infection. With no licensed vaccine, early diagnosis of dengue infection is critical for clinical management and patient survival. Detection of DENV non-structural protein 1 (NS1) is a clinically accepted biomarker for the early detection of DENV infection. Unfortunately, virtually all of the laboratory and commercial DENV NS1 diagnostic methods require a blood draw for sample analysis, limiting point-of-care diagnostics and decreases patient willingness. Alternatively, NS1 in human saliva has been identified for the potential early diagnosis of DENV infection. The collection of saliva is simple, non-invasive, painless, and inexpensive, even by minimally trained personnel. In this study, we present a label-free chemiresistive immunosensor for the detection of the DENV NS1 protein utilizing a network of single-walled carbon nanotubes functionalized with anti-dengue NS1 monoclonal antibodies. NS1 was successfully detected in adulterated artificial human saliva over the range of clinically relevant concentrations with high sensitivity and selectivity. It has potential application in clinical diagnosis and the ease of collection allows for self-testing, even within the home.

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Section: Resultsmentioning

confidence: 99%

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Wasik

Mulchandani

Yates

2018

Sensors

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“…ese materials can be cells, viruses, DNA, exosomes, blood, etc. Many techniques such as scanning tunneling microscopy (STM) [11], nanotechnology [12], carbon nanotube networks [13,14], microstrip cavity resonant measurement [15], dielectrophoretic impedance measurement (DEPIM) [16,17] are there to detect and identify these viruses. Moreover, for DNA characterization, there are various techniques such as carbon nanoparticles (CNPs) [18][19][20], field effect transistors (FETs) [21,22], cyclic voltammetry [23,24], molecular wire [25,26], graphene quantum [27,28], and dielectrophoresis [29,30].…”

Section: Electrical Characterizationmentioning

confidence: 99%

Cells Electrical Characterization: Dielectric Properties, Mixture, and Modeling Theories

Nasir

Ahmad

2020

Journal of Engineering

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Electrical detection of biotic materials and their properties is always crucial in Biomedical Engineering. It emphasizes elaborate analysis of a certain disease diagnosis using electrical means which includes the study of electrical properties followed by detection, identification, and quantification of biotic entities. Proper and early detection of cells, which can be normal or cancerous, holds never-ending demand. This review emphasizes the electrical characterization methods in modeling and classification of cell properties. Moreover, standard methods have been highlighted and discussed to yield performance analysis. This study suggests that techniques presented for single-cell analysis (SCA) are always precise and hold great scope compared to those for cell mixture. SCA plays a promising role in disease diagnosis and treatment planning, as it not only detects the cells but also helps in identification. Distinct electrical-based cell detection techniques are highlighted, along with the pros and cons of various SCA devices. The content, in terms of methodologies and available techniques, of this review paper is useful to attract researchers working in this area.

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“…Recognition of the dengue virus in a viral culture supernatant is as sensitive as the corresponding viral titer in a phosphate buffer in spite of using growth media and Vero cell lysate. The biosensor exhibited sensitivity within a range of clinical relevance for humans and infected A. aegypti [ 87 ]. A report indicates a novel procedure for detecting, classifying and antibody screening of the dengue virus on the basis of electrochemical impedance spectroscopy.…”

Section: Application Of Carbon Nanomaterials For Viral Diagnosismentioning

confidence: 99%

“…6 a) Functionalization schematic representation of the fabrication of the chemiresistor used for detection of Dengue virus. Reprinted with the permission of Ref [ 87 ]. b) Schematic illustration of Raman immunoassay based on GO-GNRs.…”

Section: Application Of Carbon Nanomaterials For Viral Diagnosismentioning

confidence: 99%

Application of carbon nanomaterials in human virus detection

Ehtesabi

2020

Journal of Science: Advanced Materials and Devices

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Human-pathogenic viruses are still a chief reason for illness and death on the globe, as epitomized by the COVID-19 pandemic instigated by a coronavirus in 2020. Multiple novel sensors have been invented because diseases must be detected and diagnosed as early as possible, and recognition methods have to be carried out with minimal invasivity. Sensors have been particularly developed focusing on miniaturization by the use of nanomaterials for fabricating nanosensors. The nano-sized nature of nanomaterials and their exclusive optical, electronical, magnetical, and mechanical attributes can enhance patient care through the use of sensors with minimal invasivity and extreme sensitivity. Amongst the nanomaterials utilized for fabricating nano-sensors, carbon-based nanomaterials are promising as these sensors respond better to signals in various sensing settings. This review provides an overview of the recent developments in carbon nanomaterial-based biosensors for viral recognition based on the biomarkers that arise from the infection, the nucleic acids from the viruses, and the entire virus. The role of carbon nanomaterials is highlighted by the improvement of sensor and recognition functionality. The Dengue virus, Ebola virus, Hepatits virus, human immunodeficiency virus (HIV), influenza virus, Zika virus and Adenovirus are the virus types reviewed to illustrate the implementation of the techniques. Finally, the drawbacks and advantages of carbon nanomaterial-based biosensors for viral recognition are identified and discussed.

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A heparin-functionalized carbon nanotube-based affinity biosensor for dengue virus

Cited by 84 publications

References 47 publications

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Cells Electrical Characterization: Dielectric Properties, Mixture, and Modeling Theories

Application of carbon nanomaterials in human virus detection

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