A Hemolytic Modification of the Hemagglutination Test for Antibodies Against Tubercle Bacillus Antigens

Agglutination of Sensitized Sheep Erythrocytes and Collodion Particles by Tuberculous and Normal Sera 1

“…Results are summarized in Table I. It will be noted that the percentage of high titers (1: 8 and over [3] or 1: 16 and over [12]) in the tuberculous group was higher than in the nontuberculous group by both methods and with both antigens. Differences between non-tuberculous and tuberculous sera were greater if 1: 16 were accepted as the "significant" titer.…”

Induction of Delayed Hypersensitivity in Guinea Pigs With Old Tuberculin or Purified Protein Derivative

“…Meanwhile, no significant increase of skin sensitivity was observed in the group sensitized with 1,000-fold dilution of OT. During 4 weeks, the circulating antibodies as determined by hemagglutination and hemolysis according to the method of Middlebrook-Dubos (1948, 1950 did not increase significantly in any group. Finally, rabbits were employed as experimental animals.…”

Hemagglutinins and Hemolysins for Erythrocytes Sensitized With Tuberculin in Pulmonary Tuberculosis 1

“…Middlebrook (2) came to the conclusion that human anti-tuberculin hemagglutinin and hemolysin were not identical since their respective titers varied at random in a small group of immune sera.…”

“…The recent discovery by Middlebrook (1,2) that sera from tuberculous animals and human beings are capable of agglutinating and lysing erythrocytes previously treated with extracts of tubercle bacilli has aroused considerable interest. In a brief period of time several clinical evaluations of the hemagglutination test have appeared in print (3,4).…”