A hemin-based molecularly imprinted polymer (MIP) grafted onto a glassy carbon electrode as a selective sensor for 4-aminophenol amperometric

Neto, José de Ribamar Martins; Santos, Wilney de Jesus Rodrigues; Lima, Phabyanno Rodrigues; Tanaka, Sônia Maria Carvalho Neiva; Tanaka, Auro Atsushi; Kubota, Lauro T.

doi:10.1016/j.snb.2010.12.010

Cited by 67 publications

(16 citation statements)

References 30 publications

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“…Electrochemical biosensors are widely synthesized by electropolymerization of such monomers as pyrrole [101], aniline [104], o -phenylenediamine (o-PD) [125,126], phenol [32], aminophenol [110,111,127] and EDOT [128,129], self-assembled monolayers (SAMs) [2,130,131] and sol-gel [93] materials.…”

Section: Electrochemical Transductionmentioning

confidence: 99%

Imprinting Technology in Electrochemical Biomimetic Sensors

Frasco

Truta

Sales

et al. 2017

Sensors

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Biosensors are a promising tool offering the possibility of low cost and fast analytical screening in point-of-care diagnostics and for on-site detection in the field. Most biosensors in routine use ensure their selectivity/specificity by including natural receptors as biorecognition element. These materials are however too expensive and hard to obtain for every biochemical molecule of interest in environmental and clinical practice. Molecularly imprinted polymers have emerged through time as an alternative to natural antibodies in biosensors. In theory, these materials are stable and robust, presenting much higher capacity to resist to harsher conditions of pH, temperature, pressure or organic solvents. In addition, these synthetic materials are much cheaper than their natural counterparts while offering equivalent affinity and sensitivity in the molecular recognition of the target analyte. Imprinting technology and biosensors have met quite recently, relying mostly on electrochemical detection and enabling a direct reading of different analytes, while promoting significant advances in various fields of use. Thus, this review encompasses such developments and describes a general overview for building promising biomimetic materials as biorecognition elements in electrochemical sensors. It includes different molecular imprinting strategies such as the choice of polymer material, imprinting methodology and assembly on the transduction platform. Their interface with the most recent nanostructured supports acting as standard conductive materials within electrochemical biomimetic sensors is pointed out.

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Section: Electrochemical Transductionmentioning

confidence: 99%

Imprinting Technology in Electrochemical Biomimetic Sensors

Frasco

Truta

Sales

et al. 2017

Sensors

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“…19 Neto et al demonstrated the performance of a heminmodified molecularly imprinted polymer (MIP) used as an amperometric sensor for the detection of 4-AP. 20 Shiroma et al developed a sensitive microfluidic paper-based device for electrochemical detection of paracetamol and 4-AP. 21 De Bleye employed a quantified 4-AP in a pharmaceutical formulation containing acetaminophen using surface-enhanced Raman scattering.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a reduced graphene oxide/poly-l-glutathione nanocomposite for electrochemical detection of 4-aminophenol in orange juice samples

et al. 2015

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A novel electrochemical sensor, 4-aminophenol (4-AP), was generated by electrodeposition of poly-L-glutathione (P-L-GSH) onto reduced graphene oxide (RGO) to form a RGO/P-L-GSH composite matrix on a glassy carbon electrode (RGO/P-L-GSH/GCE). The morphology and structure of RGO/P-L-GSH modified GCE were characterized by scanning electron microscopy (SEM), FT-IR spectroscopy and electrochemical impedance spectroscopy. As an electrochemical sensor, the RGO/P-L-GSH modified GCE composite exhibited strong catalytic activity toward the oxidation of 4-AP by cyclic voltammetry (CV) and chronoamperometry.Furthermore, the electrochemical sensor exhibited an excellent current response for 4-AP over a wide linear range of 0.4 to 200 µM (R 2 = 0.9934) with a lower detection limit (LOD ) of 0.03 µM (S/N = 3), as well as a sensitivity of 27.2 (µA µM -1 cm -2 ) and excellent selectivity, reproducibility and stability. The electrochemical sensor demonstrated good anti-interference ability in the presence of glucose, fructose, paracetamol, ethanol, l-isoluecine, l-histidine, lcysteine, dopamine, l-serine, l-tyrosine, phenylalanine, urea, hydrogen peroxide, K + , Ca 2+ , Na + , F -, SO 4 2-, Cu 2+ , Fe 3+ , Zn 2+ , SO 4 2-, SO 3 2-, Cl -, folic acid and ascorbic acid. The proposed electrochemical method was successfully applied to produce a sensor for the detection of 4-AP in juice and water samples. sensitivity and very low LOD for amperometric determination of 4-AP when compared to most other sensors reported in recent years.The 4-AP sensor was also applied for analysis of juice and water samples, and the results showed it was useful in practical applications. Experimental Section ReagentsThe graphite (powder, <20 lm), L-glutathione, 4-aminophenol, Na 2 HPO 4 and NaH 2 PO 4 , were received from the Sigma ChemicalCompany. In addition a 0.05 M phosphate buffer solution (PBS, pH 7) was used as the supporting electrolyte. Other chemicals were of analytical reagent grade, and all aqueous solutions were prepared with double distilled water for use in the experiments. ApparatusAll the electrochemical measurements were executed on a CHI 405A electrochemical workstation (Shanghai Chenhua Instrument, China).A conventional three-electrode system was used with an RGO/P-L-GSH film modified electrode as the working electrode, a platinum wire as the auxiliary electrode and a saturated Ag/AgCl as the reference electrode. Electrochemical impedance spectroscopy (EIS) was carried out in a frequency range of 0.

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“…The MIP column has a large number of "separation plates" which results in a remarka- bly low cross-reactivity towards structurally similar substances. Later a glassy carbon electrode was covered with a suspension of a heme-based bulk MIP and it responded linearly to p-aminophenol with a LOD of 3 mM and no interfering signals for o-aminophenol, catechol and guaiacol [14].…”

Section: Catalytically Active Mip Architecturesmentioning

confidence: 99%

Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

et al. 2015

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Hybrid architectures which combine a MIP with an immobilized “affinity ligand” or a biocatalyst sum up the advantages of both components. In this paper, hybrid architectures combining a layer of a molecularly imprinted electropolymer with a mini‐enzyme or a self‐assembled monolayer will be presented. (i) Microperoxidase‐11 (MP‐11) catalyzed oxidation of the drug aminopyrine on a product‐imprinted sublayer: The peroxide dependent conversion of the analyte aminopyrine takes place in the MP‐11 containing layer on top of a product‐imprinted electropolymer on the indicator electrode. The hierarchical architecture resulted in the elimination of interfering signals for ascorbic acid and uric acid. An advantage of the new hierarchical structure is the separation of MIP formation by electropolymerization and immobilization of the catalyst. In this way it was for the first time possible to integrate an enzyme with a MIP layer in a sensor configuration. This combination has the potential to be transferred to other enzymes, e.g. P450, opening the way to clinically important analytes. (ii) Epitope‐imprinted poly‐scopoletin layer for binding of the C‐terminal peptide and cytochrome c (Cyt c): The MIP binds both the target peptide and the parent protein almost eight times stronger than the non‐imprinted polymer with affinities in the lower micromolar range. Exchange of only one amino acid in the peptide decreases the binding by a factor of five. (iii) MUA‐poly‐scopoletin MIP for cytochrome c: Cyt c bound to the MIP covered gold electrode exhibits direct electron transfer with a redox potential and rate constant typical for the native protein. The MIP cover layer suppresses the displacement of the target protein by BSA or myoglobin. The combination of protein imprinted polymers with an efficient electron transfer is a new concept for characterizing electroactive proteins such as Cyt c. The competition with other proteins shows that the MIP binds its target Cyt c preferentially and that molecular shape and the charge of protein determine the binding of interfering proteins.

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A hemin-based molecularly imprinted polymer (MIP) grafted onto a glassy carbon electrode as a selective sensor for 4-aminophenol amperometric

Cited by 67 publications

References 30 publications

Imprinting Technology in Electrochemical Biomimetic Sensors

Imprinting Technology in Electrochemical Biomimetic Sensors

Preparation of a reduced graphene oxide/poly-l-glutathione nanocomposite for electrochemical detection of 4-aminophenol in orange juice samples

Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

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