A helping hand: How vinculin contributes to cell-matrix and cell-cell force transfer

“…vinculin binding sites in talin rod (115,160,206), ii) disappearance of binding sites due to mechanical load (DLC1 -talin, (178, 252)), iii) phosphorylation of mechano-exposed regions within proteins (p130Cas) (385), iv) and proteolytic cleavage of partially unfolded proteins (4). According to the motor-clutch model, integrin-ligand affinity is a parameter that might shift the onset of mechanotransduction from 5 kPa to softer or stiffer substrates.…”

Section: Yap/tazmentioning

confidence: 99%

Cell Adhesion by Integrins

Bachmann

Kukkurainen

Hytönen

et al. 2019

Physiological Reviews

283

223

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Integrins are heterodimeric cell surface receptors ensuring the mechanical connection between cells and the extracellular matrix. In addition to the anchorage of cells to the extracellular matrix, these receptors have critical functions in intracellular signaling, but are also taking center stage in many physiological and pathological conditions. In this review, we provide some historical, structural, and physiological notes so that the diverse functions of these receptors can be appreciated and put into the context of the emerging field of mechanobiology. We propose that the exciting journey of the exploration of these receptors will continue for at least another new generation of researchers.

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“…34 Vinculin is a cytoplasmic actin-binding protein that participates in the linkage of integrins to the actin cytoskeleton, governing cellmatrix interactions and cell−cell adhesions. 35 It is expressed in terminally differentiated (contractile) muscle cells at the costameres, structural functional components of striated muscle cells. Interestingly, all markers were detected at significantly higher levels in C2C12 cells within rGO-coated fibers.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In skeletal muscle, β1-integrin is involved in muscle cell migration, fusion, and sarcomere assembly . Vinculin is a cytoplasmic actin-binding protein that participates in the linkage of integrins to the actin cytoskeleton, governing cell-matrix interactions and cell–cell adhesions . It is expressed in terminally differentiated (contractile) muscle cells at the costameres, structural functional components of striated muscle cells.…”

Section: Resultsmentioning

confidence: 99%

Customizable Composite Fibers for Engineering Skeletal Muscle Models

Fallahi

Yazdi

Serex

et al. 2019

ACS Biomater. Sci. Eng.

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Engineering tissue-like scaffolds that can mimic the microstructure, architecture, topology, and mechanical properties of native tissues while offering an excellent environment for cellular growth has remained an unmet need. To address these challenges, multicompartment composite fibers are fabricated. These fibers can be assembled through textile processes to tailor tissue-level mechanical and electrical properties independent of cellular level components. Textile technologies also allow control of the distribution of different cell types and the microstructure of fabricated constructs and the direction of cellular growth within the 3D microenvironment. Here, we engineered composite fibers from biocompatible cores and biologically relevant hydrogel sheaths. The fibers are mechanically robust to being assembled using textile processes and could support adhesion, proliferation, and maturation of cell populations important for the engineering of skeletal muscles. We also demonstrated that the changes in the coating of the multicompartment fibers could potentially enhance myogenesis in vitro.

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A helping hand: How vinculin contributes to cell-matrix and cell-cell force transfer

Cited by 9 publications

References 53 publications

Vinculin regulates directionality and cell polarity in two- and three-dimensional matrix and three-dimensional microtrack migration

Vinculin regulates directionality and cell polarity in two- and three-dimensional matrix and three-dimensional microtrack migration

Cell Adhesion by Integrins

Customizable Composite Fibers for Engineering Skeletal Muscle Models

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