A helpers' guide to infection

Gebhardt, Thomas; Carbone, Federico

doi:10.1038/462418a

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…As the innate immunity yields to adaptive immunity, the associated migration of lymphocytes occurs within days (53–55), with CD4 + T-cell infiltration preceding CD8 + T-cells (13, 14, 56). Indeed, the presence of CD4 + T-cells seems to be critical for the subsequent recruitment of activated CD8 + T-cells (56, 57). On the other hand, B-cells are not typically recruited to sites of acute inflammation (14, 15, 58, 59).…”

Section: Discussionmentioning

confidence: 99%

Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology

Silva

Fung

Donnelly

et al. 2017

The Journal of Immunology

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Both host defense and immunopathology are shaped by the ordered recruitment of circulating leukocytes to affected sites, a process initiated by binding of blood-borne cells to E-selectin displayed at target endothelial beds. Accordingly, knowledge of the expression and function of leukocyte E-selectin ligands is key to understanding the tempo and specificity of immunoreactivity. Here, we performed E-selectin adherence assays under hemodynamic flow conditions coupled with flow cytometry and western blot analysis to elucidate the function and structural biology of glycoprotein E-selectin ligands expressed on human peripheral blood mononuclear cells (PBMCs). Circulating monocytes uniformly express high levels of the canonical E-selectin binding determinant sLeX and display markedly greater adhesive interactions with E-selectin than do circulating lymphocytes, which exhibit variable E-selectin binding among CD4+ and CD8+ T-cells but no binding by B-cells. Monocytes prominently present sLeX decorations on an array of protein scaffolds including PSGL-1, CD43, and CD44 (rendering the E-selectin ligands CLA, CD43E, and HCELL, respectively), and B-cells altogether lack E-selectin ligands. Quantitative PCR gene expression studies of glycosyltransferases that regulate display of sLeX reveal high transcript levels among circulating monocytes and low levels among circulating B-cells, and, commensurately, cell surface α(1,3)-fucosylation reveals that acceptor sialyllactosaminyl glycans convertible into sLeX are abundantly expressed on human monocytes yet are relatively deficient on B-cells. Collectively, these findings unveil distinct cell-specific patterns of E-selectin ligand expression among human PBMCs, indicating that circulating monocytes are specialized to engage E-selectin and providing key insights into the molecular effectors mediating recruitment of these cells at inflammatory sites.

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Section: Discussionmentioning

confidence: 99%

Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology

Silva

Fung

Donnelly

et al. 2017

The Journal of Immunology

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“…Effector memory T cells (T EM ) migrate from the blood into the peripheral nonlymphoid tissues as they express the inflamed tissue homing receptors and not CD62L and CCR7. Studies in humans showed that these cells very efficiently protect the peripheral tissues by production of effector cytokines such as IFN- γ and IL-4 [ 79 – 81 ]. Recently, the existence of tissue resident memory T cells (T RM ) has been reported not only in the human skin [ 82 ] and lungs [ 83 , 84 ], but also in murine lungs [ 83 , 84 ], central nervous system [ 85 ], bone marrow (BM) [ 86 ], and intestine [ 87 , 88 ].…”

Section: Does Cd69 Expression Indicate Resident Memory T Cells?mentioning

confidence: 99%

CD69 Is the Crucial Regulator of Intestinal Inflammation: A New Target Molecule for IBD Treatment?

Radulovic

Niess

2015

Journal of Immunology Research

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CD69 has been identified as an early activation marker of lymphocytes. However, recent work has indicated that CD69 plays an essential role for the regulation of inflammatory processes. Particularly, CD69 is highly expressed by lymphocytes at mucosal sites being constantly exposed to the intestinal microflora (one of the nature's most complex and most densely populated microbial habitats) and food antigens, while only a small number of circulating leukocytes express this molecule. In this review we will discuss the role of CD69 in mucosal tissue and consider CD69 as a potential target for the development of novel treatments of intestinal inflammation.

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