A heat shock element in the phosphoglycerate kinase gene promoter of yeast

Piper, Peter W.; Curran, Brendan; Davies, Mark W.; Hirst, K.; Lockheart, Alan; Ogden, Jill E.; Stanway, Clive; Kingsman, Alan J.; Kingsman, Susan M.

doi:10.1093/nar/16.4.1333

Cited by 44 publications

(24 citation statements)

References 25 publications

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“…It has been suggested that retinol dehydrogenase 13 (TC11759) protects mitochondria from oxidative stress in humans [72]. Yeast phosphoglycerate kinase (PGK; TC16651) has been shown to be induced following heat-shock, and to contain a heat-shock promoter element [73,74]. In addition, the PGK promoter has been shown to be induced following sub-lethal oxidative stress exposure [75].…”

Section: Resultsmentioning

confidence: 99%

Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni

Aragon

Imani

Blackburn

et al. 2008

Molecular and Biochemical Parasitology

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The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host immune system. The most common treatment for schistosomiasis is the anti-helminthic drug praziquantel. Its effectiveness, however, is limited due to its inability to kill schistosomes 2 -4 weeks post-infection. Clearly there is a need for new antischistosomal drugs. We hypothesize that gene products expressed as part of a protective response against heat and/or oxidative stress are potential therapeutic targets for future drug development. Using a 12,166 element oligonucleotide microarray to characterize Schistosoma mansoni genes induced by heat and oxidative stress we found that 1,878 S. mansoni elements were significantly induced by heat stress. These included previously reported heat-shock genes expressing homologs of HSP40, HSP70 and HSP86. One thousand and one elements were induced by oxidative stress including those expressing homologs of superoxide dismutase, glutathione peroxidase and aldehyde dehydrogenase. Seventy-two elements were common to both stressors and could potentially be exploited in the development of novel anti-schistosomal therapeutics.

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Section: Resultsmentioning

confidence: 99%

Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni

Aragon

Imani

Blackburn

et al. 2008

Molecular and Biochemical Parasitology

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“…These include two glycolytic enzymes (enolase [17] and phosphoglycerate kinase [32]) and two UV-inducible genes that also show heat inducibility (26). The …”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae.

Nicolet¹,

Craig²

1989

Mol. Cell. Biol.

227

186

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We have isolated a gene from the yeast Saccharomyces cerevisiae that encodes a 2.0-kilobase heat-inducible mRNA. This gene, which we have designated STII, for stress inducible, was also induced by the amino acid analog canavanine and showed a slight increase in expression as cells moved into stationary phase. The STII gene encodes a 66-kilodalton protein, as determined from the sequence of the longest open reading frame. The putative STII protein, as identified by two-dimensional gel electrophoresis, migrated in the region of 73 to 75 kilodaltons as a series of four isoforms with different isoelectric points. STII is not homologous to the other conserved HSP70 family members in yeasts, despite similarities in size and regulation. Cells carrying a disruption mutation of the STI) gene grew normally at 30°C but showed impaired growth at higher and lower temperatures. Overexpression of the STII gene resulted in substantial trans-activation of SSA4 promoterreporter gene fusions, indicating that STII may play a role in mediating the heat shock response of some HSP70 genes.Cells from early every organism thus far tested respond to an increase in temperature by inducing synthesis of a stereotypical set of proteins, the so-called heat shock proteins. Synthesis of these proteins is also induced by a number of other stimuli that stress the cell, indicating that the response is a general one to organismal (and cellular) trauma. The most abundant of the heat shock proteins found after temperature upshift is a 70-kilodalton (kDa) protein called hsp70. hsp70 is the most highly conserved of all heat shock proteins, showing between 60 and 78% identity among eucaryotes and 40 to 50% identity between Escherichia coli hsp70, the dnaK gene product, and the corresponding eucaryotic proteins (reviewed in reference 22).Among the best characterized of the HSP70 genes are those from the yeast Saccharomyces cerevisiae. Eight genes related to HSP70 have been isolated as recombinant DNA clones in our laboratory. These eight genes have been divided into four phenotypic subfamilies, SSA through SSD (SS denotes stress seventy related; A through D indicate the subfamilies). The expression patterns and functional interrelationships of members of the SSA subfamily (encompassing SSAJ through SSA4) have been the most thoroughly investigated. The SSAI gene is expressed at moderate levels at 23°C but is induced approximately 10-fold after a temperature shift to 37°C, whereas SSA2 is expressed at a high level at all temperatures. SSA3 and SSA4 are expressed at extremely low levels at 23°C, but after a temperature upshift the amounts of SSA3 and SSA4 mRNAs increase dramatically (9

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“…4) al., 1990) and GCRl (Henry et al, 1994). A heat-shock element is also found in the promoter region (Piper et al, 1988a). The 5' region of PGKl was examined for homology with the consensus sequences for these binding factors and for TATA sequences.…”

Section: Dna Sequence Analysismentioning

confidence: 99%

3-Phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of Candida albicans

et al. 1997

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We have used a polyclonal antiserum t o cell wall proteins of Candida albicans to isolate several clones from a cDNA i l g t l l expression library. Affinitypurified antibody prepared to the fusion protein of one clone identified a 40 kDa moiety present in cell wall extracts from both morphologies of the organism. Indirect immunof luorescence demonstrated expression of this moiety at the C. albicans cell surface. Sequencing of a pBluescript II genomic clone identified with the cDNA clone revealed an open reading frame for a 417 amino acid protein. The nucleotide sequence showed significant homology with 3-phosphoglycerate kinase (PGK) genes, with 88%, 77% and 76% nucleotide homology with the PGK genes from Candida maltosa, Sacchammyces cere visiae and Kluyveromyces lactis, respectively. The deduced amino acid sequence was consistent with this identification of the sequence as PGKI of C. albicans. This finding was confirmed by a positive immunological response of a commercially available purified PGK from S. cerevisiae with the affinity-purified antibody against the fusion protein of the cDNA clone. The presence of PGK in the cell wall was confirmed by two additional methods.Cell wall proteins were biotinylated with a derivative that does not permeate the cell membrane to distinguish extracellular from cytosolic proteins. Biotinylated PGK was detected among the biotinylated proteins obtained following streptavidin affinity chromatography. lmmunoelectron microscopy revealed that the protein was present at the outer surface of the cell membrane and cell wall as well as expected in the cytoplasm. Northern blot analysis revealed that the gene transcript was present in C. albicans cells growing under different conditions, including different media, temperatures and morphologies. Most of the enzyme activity was found in the cytosol. Low enzymic activity was detected in intact cells but not in culture filtrates. These observations confirmed that PGK is a bona fide cell wall protein of C. albicans.

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A heat shock element in the phosphoglycerate kinase gene promoter of yeast

Cited by 44 publications

References 25 publications

Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni

Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni

Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae.

3-Phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of Candida albicans

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