A healthy birth after intracytoplasmic sperm injection by using immotile testicular spermatozoa in a case with totally immotile ejaculated spermatozoa before and after Percoll gradients

Kahraman, Semra; Işık, Ahmet Zeki; Vıcdan, Kubilay; Özgür, Suat; Özgün, O.D.

doi:10.1093/humrep/12.2.292

Cited by 27 publications

(14 citation statements)

References 6 publications

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“…In azoospermic patients it is frequently observed that more immotile than motile spermatozoa are obtained in TESE. But even the use of immotile spermatozoa either from the ejaculate (Kahraman et al, 1996;Nijs et al, 1996;Vandervorst et al, 1997;Wang et al, 1997;Barros et al, 1998) or extracted from a testicular biopsy (Nijs et al, 1996;Kahraman et al, 1997;Shulman et al, 1999) has succeeded in pregnancies, revealing that immotile spermatozoa can successfully fertilize.…”

Section: Introductionmentioning

confidence: 99%

Optimizing TESE‐ICSI by laser‐assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes

et al. 2012

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SUMMARYFor most azoospermic men testicular sperm extraction (TESE) is the only treatment, however it presents challenges for the ART laboratory, as the retrieval of motile spermatozoa is difficult. In the absence of sperm movement no unequivocal distinction can be made between either dead or immotile, but vital spermatozoa. However, a single laser shot directed to the tip of the tail allows recognition of viability because the flagellum coils at the area of impact. To rank the quality and the maturity of oocytes, polarization microscopy can be used. The zona score and the visualization of the meiotic spindle correlate with implantation and pregnancy rates. We compared 65 TESE-ICSI cycles of the years 2007 and 2008 (Group 1, G1) with 58 TESE-ICSI cycles of the years 2009 and 2010 (Group 2, G2). Testicular spermatozoa were injected according to motility and morphology into selected oocytes. In G1 both, oocyte and spermatozoa were rated using light microscopy only, whereas in G2 the laser was used for sperm selection and the oocytes were rated by light and polarization microscopy. In G2 we enhanced our fertilization rate (FR) significantly in comparison to G1 (G1 42.1% vs. G2 52.7%, p < 0.001). The fertilization rate with immotile, but vital spermatozoa improved significantly when applying laser-based selection (p = 0.006). The laser selection of immotile spermatozoa and the use of polarization microscopy can enhance the FR of TESE-ICSI. No negative effect of the laser was seen on birth rates. The FR with immotile, but vital spermatozoa clearly benefits from laser selection and is a non-hazardous and safe method for the selection of viable but immotile sperm. To our knowledge this is the first report using new technology creating novel endpoints for the analysis of spermatozoa and oocytes in TESE-ICSI.

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Section: Introductionmentioning

confidence: 99%

Optimizing TESE‐ICSI by laser‐assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes

et al. 2012

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“…Successful treatment of PCD with the birth of healthy babies following embryo transfer on day three or earlier has been reported using sub-zonal insemination (SUZI) [4][5][6] and intracytoplasmic sperm injection (ICSI) [7][8][9][10][11][12][13]. The hypo-osmotic swelling (HOS) test [14] was used in conjunction with ICSI of immotile spermatozoa with success in several cases as well [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

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Purpose To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture. Materials and methods Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage. Results 100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3-17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks. Conclusions The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.

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“…Rare and sporadic forms of male infertility which • are probably caused by mutations, such as globozoospermia (Lundin et al 1994;Bourne et al 1995;Liu et al 1995;Catt et al 1996;Battaglia et al 1997;Rybouchkin et al 1997;Tejera et al 2008, Fig. 23.10) and total sperm immotility (Nijs et al 1996;Casper et al 1996;Liu et al 1997;Kahraman et al 1997;Peeraer 2004). In these rare occasions the fertilization rate after ICSI seemed to be lower than the usual, which varies between 60-70%.…”

Section: Intracytoplasmic Sperm Injection (Icsi)mentioning

confidence: 99%

Assisted Reproduction

Geyter¹,

Geyter²,

Behre³

2010

Andrology

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A healthy birth after intracytoplasmic sperm injection by using immotile testicular spermatozoa in a case with totally immotile ejaculated spermatozoa before and after Percoll gradients

Cited by 27 publications

References 6 publications

Optimizing TESE‐ICSI by laser‐assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes

Optimizing TESE‐ICSI by laser‐assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Assisted Reproduction

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