A HD-ZIP transcription factor specifies fates of multicellular trichomes via dosage-dependent mechanisms in tomato

Wu, Minliang; Chang, Jiang; Han, Xiuli; Shen, Jingyuan; Yang, Liling; Hu, Shourong; Huang, Ben-Ben; Xu, Huimin; Xu, Mengyuan; Wu, Shaopeng; Li, Pengxue; Hua, Bin; Yang, Meng‐Yin; Yang, Zhenbiao; Wu, Shuang

doi:10.1016/j.devcel.2023.01.009

Cited by 28 publications

(25 citation statements)

References 70 publications

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“…WOOLLY is a homeodomain leucine zipper IV (HD-ZIP IV) transcription factor, previously described to control type-I trichome formation (Yang et al, 2011). Combined, these findings are consistent with the recent discovery that WOOLLY actually controls different trichomes types in a dosage-dependent manner (Wu et al, 2023), and this knowledge opens the possibility for use in molecular breeding.…”

Section: Introductionsupporting

confidence: 89%

“…Wo m is a mild gain-of-function mutation in the gene WOOLLY ( Solyc02g080260 ) that increases the density of digitate trichomes (mainly types III and IV, Vendemiatti et al, 2017) and results in a hairy phenotype (Fig. 2 - Yang et al, 2011; Wu et al, 2023). This gene is located 9.16 Mb upstream of the MT- Get02 locus.…”

Section: Resultsmentioning

confidence: 99%

“…The increased density of type-IV trichome in the adult phase of Get02/Wo supports the notion that Wo can enhance type-IV trichome formation (Vendemiatti et al, 2017), and it suggests that Get loci play a role in controlling juvenility (Vendemiatti et al, 2022). Interestingly, recent research has shown that the Woolly mutant can increase the density of all digitate trichome types (Wu et al, 2023), including types I, II, III, IV and V. While types I, II and III are sparsely present in all phases of tomato development, types IV and V predominate in the juvenile and in the adult phases, respectively, with their densities showing a negative correlation throughout tomato development (Vendemiatti et al, 2017). Since type-IV and type-V trichomes differ morphologically solely by the presence of a single-cell gland at the tip of the former, they likely share the same initial developmental pathway.…”

Section: Discussionmentioning

confidence: 99%

“…Since type-IV and type-V trichomes differ morphologically solely by the presence of a single-cell gland at the tip of the former, they likely share the same initial developmental pathway. Therefore, determining the genetic identity of Get02 may contribute to our understanding of the genetic network downstream of WOOLLY in specifying different digitate trichome types (Wu et al, 2023). According to the model proposed by Wu et al (2023), elevated WOOLLY levels result in digitate trichome differentiation.…”

Section: The Woolly Mutation Plays a Pivotal Role In Increasing The D...mentioning

confidence: 99%

“…Therefore, determining the genetic identity of Get02 may contribute to our understanding of the genetic network downstream of WOOLLY in specifying different digitate trichome types (Wu et al, 2023). According to the model proposed by Wu et al (2023), elevated WOOLLY levels result in digitate trichome differentiation. In contrast, low levels lead to the formation of peltate trichomes (types VI and VII).…”

Section: The Woolly Mutation Plays a Pivotal Role In Increasing The D...mentioning

confidence: 99%

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Woollymutation withGet02locus overcomes the polygenic nature of trichome-based pest resistance in tomato

Vendemiatti,

Hernández-De Lira,

Snijders

et al. 2023

Preprint

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Type-IV glandular trichomes, which only occur in the juvenile phase of tomato development, produce acylsugars (AS) that broadly protect against arthropod herbivory. Previously, we introgressed the capacity to retain type-IV trichomes in the adult phase fromSolanum galapagenseinto the cv. Micro-Tom (MT). The resulting MT-Getline contained five loci associated with enhancing the density of type-IV trichomes in adult plants. We genetically dissected MT-Getand obtained a sub-line containing only the locus on chromosome 2 (MT-Get02). This genotype displayed about half the density of type-IV trichomes compared to the wild progenitor. However, when we stacked the gain-of-function allele ofWOOLLY, which codes for a HD-ZIP IV transcription factor, MT-Get02/Woexhibited double the number of type-IV trichomes compared toS. galapagense. This discovery corroborates previous reports positioningWOOLLYas a master regulator of trichome development. AS levels in MT-Get02/Wowere comparable to the wild progenitor, although the composition of AS types differed, especially regarding less AS with medium-length acyl chains. Agronomical parameters of MT-Get02/Wo, including yield, were comparable to MT. Pest resistance assays showed enhanced protection against whitefly, caterpillar, and the fungusSeptoria lycopersici. However, resistance levels did not reach that of the wild progenitor, suggesting the specificity of particulars AS types in the pest resistance mechanism. Our findings in trichome-mediated resistance advance the development of robust, naturally resistant tomato varieties, harnessing the potential of natural genetic variation. Moreover, by manipulating only two loci, we achieved exceptional results for a highly complex, polygenic trait, such as herbivory resistance in tomato.

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Section: Introductionsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Woolly Mutation Plays a Pivotal Role In Increasing The D...mentioning

confidence: 99%

Section: The Woolly Mutation Plays a Pivotal Role In Increasing The D...mentioning

confidence: 99%

See 3 more Smart Citations

Woollymutation withGet02locus overcomes the polygenic nature of trichome-based pest resistance in tomato

Vendemiatti,

Hernández-De Lira,

Snijders

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Morphological, phytochemical, and transcriptome analyses provide insights into the biosynthesis of monoterpenes in Monarda citriodora

Sharma¹,

Wajid²,

Fayaz³

et al. 2023

Planta

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Genome-wide characterization and expression analysis of the HD-Zip II gene family in response to drought and GA3 stresses in Nicotiana tabacum

Liu,

Zhang,

Yang

et al. 2024

Mol Biol Rep

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A HD-ZIP transcription factor specifies fates of multicellular trichomes via dosage-dependent mechanisms in tomato

Cited by 28 publications

References 70 publications

Woollymutation withGet02locus overcomes the polygenic nature of trichome-based pest resistance in tomato

Woollymutation withGet02locus overcomes the polygenic nature of trichome-based pest resistance in tomato

Morphological, phytochemical, and transcriptome analyses provide insights into the biosynthesis of monoterpenes in Monarda citriodora

Genome-wide characterization and expression analysis of the HD-Zip II gene family in response to drought and GA3 stresses in Nicotiana tabacum

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