2020
DOI: 10.1101/2020.06.29.20142349
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A handheld point-of-care system for rapid detection of SARS-CoV-2 in under 20 minutes

Abstract: The COVID-19 pandemic is a global health emergency characterized by the high rate of transmission and ongoing increase of cases globally. Rapid point-of-care (PoC) diagnostics to detect the causative virus, SARS-CoV-2, are urgently needed to identify and isolate patients, contain its spread and guide clinical management. In this work, we report the development of a rapid PoC diagnostic test (< 20 min) based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and semiconductor technolog… Show more

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Cited by 19 publications
(27 citation statements)
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“… 43 Altogether the 51 studies analyzed 18,923 persons, of them 10,181 persons (22 studies) before COVID-19 and 8,742 persons for SARS-CoV-2 (29 studies). 7 , 9 , 13 , 14 , 17 , 18 , 19 , 22 , 23 , 25 , 26 , 27 , 34 , 35 , 36 , 38 , 39 , 41 , 43 , 45 , 46 , 47 , 50 , 51 , 53 , 54 , 55 , 56 The studies evaluated mostly patients with non-specific ILI or suspected COVID-19, the latter including both upper ARTI and pneumonia. Five studies included only children <2 years 16 , 21 , 31 , 32 , 48 , 25 studies included a mixed age range and 21 did not address patients' age.…”
Section: Resultsmentioning
confidence: 99%
“… 43 Altogether the 51 studies analyzed 18,923 persons, of them 10,181 persons (22 studies) before COVID-19 and 8,742 persons for SARS-CoV-2 (29 studies). 7 , 9 , 13 , 14 , 17 , 18 , 19 , 22 , 23 , 25 , 26 , 27 , 34 , 35 , 36 , 38 , 39 , 41 , 43 , 45 , 46 , 47 , 50 , 51 , 53 , 54 , 55 , 56 The studies evaluated mostly patients with non-specific ILI or suspected COVID-19, the latter including both upper ARTI and pneumonia. Five studies included only children <2 years 16 , 21 , 31 , 32 , 48 , 25 studies included a mixed age range and 21 did not address patients' age.…”
Section: Resultsmentioning
confidence: 99%
“…The copyright holder for this this version posted September 25, 2020. ; https://doi.org/10.1101/2020.09.18.20195370 doi: medRxiv preprint Kingdom testing program more than seven months after the SARS-CoV-2 pandemic was declared a public health emergency of international concern by the WHO. Further expansion of testing to include screening of asymptomatic individuals, which may be needed to prevent SARS-CoV-2 circulation, would require a significant further increase in testing capacity 1,2 . In the UK, clinical laboratories have struggled to expand conventional RT-PCR workflows to meet the demand for SARS-CoV-2 testing, and many have explored alternative methods that would be more scalable or allow near-patient use 3,4 . At the Oxford University Hospitals NHS Foundation Trust (OUH) and Sheffield Teaching Hospitals NHS Foundation Trust (STH), we evaluated LamPORE, a novel diagnostic platform for SARS-CoV-2 that combines loopmediated isothermal amplification (LAMP) with nanopore sequencing 5 .…”
Section: Resultsmentioning
confidence: 99%
“…Arguably, LAMP is most valuable at the POC because it is fast, simple, and robust; and there are a few examples in the literature of POC platforms that incorporate RT-LAMP to detect SARS-CoV-2 (Table 2). Rodriguez-Manzano et al designed a lab on a chip platform capable of detecting 8 copies/µL of SARS-CoV-2 [132]. Although this handheld platform has a 91% sensitivity and 100% specificity when compared to RT-qPCR, RNA extraction is necessary prior to RT-LAMP [132].…”
Section: Lamp For Covid-19 Detectionmentioning
confidence: 99%
“…Rodriguez-Manzano et al designed a lab on a chip platform capable of detecting 8 copies/µL of SARS-CoV-2 [132]. Although this handheld platform has a 91% sensitivity and 100% specificity when compared to RT-qPCR, RNA extraction is necessary prior to RT-LAMP [132]. Garneret et al describe their POC platform that utilizes membranes for RNA extraction and amplification [133].…”
Section: Lamp For Covid-19 Detectionmentioning
confidence: 99%