A hand-held, real-time, AI-assisted capillary convection PCR system for point-of-care diagnosis of African swine fever virus

Miao, Guijun; Jiang, Xiaodan; Yang, Dianlong; Fu, Qiang; Zhang, Lulu; Ge, Shengxiang; Ye, Xiangzhong; Xia, Ningshao; Qian, Shizhi; Qiu, Xianbo

doi:10.1016/j.snb.2022.131476

Cited by 6 publications

(1 citation statement)

References 40 publications

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“…Previously, we have developed different CPCR reactors (Qiu et al 2017a , 2017b , 2017c , 2019 ; Miao et al 2022 ) for rapid nucleic acid amplification, which more focused on CPCR reactor itself. Different from our previous work, this paper demonstrated a combined platform for rapid ‘sample-in, answer-out’ virus detection by taking advantage of both convective PCR and microfluidics.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic system for rapid nucleic acid analysis based on real-time convective PCR at point-of-care testing

Jiang

Zou

et al. 2022

Microfluid Nanofluid

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A microfluidic system for rapid nucleic acid analysis based on real-time convective PCR is developed. To perform ‘sample-in, answer-out’ nucleic acid analysis, a microfluidic chip is developed to efficiently extract nucleic acid, and meanwhile convective PCR (CPCR) is applied for rapid nucleic acid amplification. With an integrated microfluidic chip consisting of reagent pre-storage chambers, a lysis & wash chamber, an elution chamber and a waste chamber, nucleic acid extraction based on magnetic beads can be automatically performed for a large size of test sample within a limited time. Based on an easy-to-operate strategy, different pre-stored reagents can be conveniently released for consecutive reaction at different steps. To achieve efficient mixing, a portable companion device is developed to introduce properly controlled 3-D actuation to magnetic beads in nucleic acid extraction. In CPCR amplification, PCR reagent can be spontaneously and repeatedly circulated between hot and cool zones of the reactor for space-domain thermal cycling based on pseudo-isothermal heating. A handheld real-time CPCR device is developed to perform nucleic acid amplification and in-situ detection. To extend the detection throughput, multiple handheld real-time CPCR devices can be grouped together by a common control system. It is demonstrated that influenza A (H1N1) viruses with the reasonable concentration down to 1.0 TCID 50 /ml can be successfully detected with the microfluidic system.

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