A hand-held device for rapid single tube detection of hepatitis-C virus

Jogezai, Naqeebullah; Shabbir, Muhammad Imran

doi:10.1039/c8ay00802g

Cited by 15 publications

(3 citation statements)

References 40 publications

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“…According to previous reports, the change in pH value is normally around 2.5 units when the LAMP reaction occurred in a non-buffered solution [ 30 ]. Since the pH value of the LAMP master mix used in this study is around 8.5, the potentiometric response of the E-INAATs device was characterized by using TE buffer with a pH value from 6.0 to 8.5.…”

Section: Resultsmentioning

confidence: 99%

Real-time monitoring of isothermal nucleic acid amplification on a smartphone by using a portable electrochemical device for home-testing of SARS-CoV-2

Gao

et al. 2022

Analytica Chimica Acta

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Section: Resultsmentioning

confidence: 99%

Real-time monitoring of isothermal nucleic acid amplification on a smartphone by using a portable electrochemical device for home-testing of SARS-CoV-2

Gao

et al. 2022

Analytica Chimica Acta

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“…Several devices to simplify the amplification and detection of HCV RNA using RT-LAMP have been developed. These include portable battery-operated LED heaters integrated with ion sensors to detect nucleic acid amplification and heat-block-compatible microfluidics devices that integrate amplification and lateral flow detection of HCV RNA as low as 398 copies per reaction in under 40 min [152,153]. A microfluidics device that is capable of integrating magnetic-bead-based nucleic acids extraction from plasma samples with RT-LAMP and colorimetric detection was reported to be able to detect 500 HCV virions per mL in 45 min [33].…”

Section: Hepatits C Virusmentioning

confidence: 99%

Point-of-Care Testing for Hepatitis Viruses: A Growing Need

Pauly,

Ganova-Raeva

2023

Life

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Viral hepatitis, caused by hepatitis A virus (HAV), hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus (HDV), or hepatitis E virus (HEV), is a major global public health problem. These viruses cause millions of infections each year, and chronic infections with HBV, HCV, or HDV can lead to severe liver complications; however, they are underdiagnosed. Achieving the World Health Organization’s viral hepatitis elimination goals by 2030 will require access to simpler, faster, and less expensive diagnostics. The development and implementation of point-of-care (POC) testing methods that can be performed outside of a laboratory for the diagnosis of viral hepatitis infections is a promising approach to facilitate and expedite WHO’s elimination targets. While a few markers of viral hepatitis are already available in POC formats, tests for additional markers or using novel technologies need to be developed and validated for clinical use. Potential methods and uses for the POC testing of antibodies, antigens, and nucleic acids that relate to the diagnosis, monitoring, or surveillance of viral hepatitis infections are discussed here. Unmet needs and areas where additional research is needed are also described.

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“…The need for a heat source, which is dependent on electrical power, makes it unsuitable for very low‐resource setting environments. To solve this problem, chemically heated 44,63–65 or battery‐powered heat sources 66–68 were suggested. However, they are not ideal solutions as they can process a very limited number of samples at once.…”

Section: Assured: a Closer Lookmentioning

confidence: 99%

ASSURED‐compliant point‐of‐care diagnostics for the detection of human viral infections

Naseri

Ziora

Simon

et al. 2021

Reviews in Medical Virology

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Viral disease outbreaks have been always a threat to global public health making affordable, rapid and accurate diagnostics highly important tools to slow down the spread of viruses and decrease the mortality rate. Point‐of‐care (POC) diagnostics have emerged as a strong tool for the diagnosis of viral infections, especially in countries where health‐care systems are inadequate to provide proper services to all citizens. According to the World Health Organization, an ideal POC diagnostic must be Affordable, Sensitive, Specific, User‐friendly, Rapid/Robust, Equipment‐free and Deliverable (ASSURED). This review surveys carefully each ASSURED criterion and identifies where existing viral POC diagnostics fail to meet these criteria. Given the widespread concern with plastic pollution, we also propose the addition of 'disposability' to the existing ASSURED criteria and consider the letter “D” as the representative of both Deliverable and Disposable. Next, the POC tests used for the diagnosis of several common human viral infections which met all the ASSURED criteria (ASSURED‐compliant) are described in detail. Finally, the future of ASSURED‐compliant POC diagnostics, capable of generating comparable results to the viral diagnostic gold standards, is discussed.

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A hand-held device for rapid single tube detection of hepatitis-C virus

Abstract: A Single Tube Analysis system using LAMP, LED and ION-sensing (STALLION) for pathogen derived RNA/DNA.

Cited by 15 publications

References 40 publications

Real-time monitoring of isothermal nucleic acid amplification on a smartphone by using a portable electrochemical device for home-testing of SARS-CoV-2

Real-time monitoring of isothermal nucleic acid amplification on a smartphone by using a portable electrochemical device for home-testing of SARS-CoV-2

Point-of-Care Testing for Hepatitis Viruses: A Growing Need

ASSURED‐compliant point‐of‐care diagnostics for the detection of human viral infections

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