A hairpin DNA-fueled nanoflare for simultaneous illumination of two microRNAs in drug-induced nephrotoxic cells with target catalytic recycling amplification

Abstract

A hairpin DNA-fueled nanoflare for the simultaneous and sensitive detection of two drug-induced nephrotoxicity-related miRNAs with target catalytic recycling amplification.

Advances in Biosensor Technologies for Acute Kidney Injury

“…36 For instance, miRNA-21 and miRNA-200c are also associated with the drug-induced AKI, and accordingly, novel hairpin DNAfueled nanoflares have been introduced to determine their levels by using target catalytic recycling amplification. 144 Briefly, two hairpin DNA (H1 for miR-21 and H2 for miR-200c) were labeled with FAM and cyanine 5 (Cy5), and they were immobilized on the surface of AuNPs. The strategy was to hybridize the double strands, including H1-miR-21 and H2-miR-200c, when miRNA was encountered with nanoflares, and eventually, these interactions resulted in two-colored fluorescence signals.…”

“…The LOD values of these strategies were 18.1 and 21.1 pM for miRNA-21 and miRNA-200c, respectively. 144 In another study determining the levels of miRNA-21, a personal glucose meter (PGM) was developed. 145 The platform constituted streptavidin-immobilized magnetic beads (MBs)-coated with RNA-cleaving DNAzyme strands through biotin−avidin interactions.…”

“…As aforementioned, different miRNA types (e.g., miR-494 and miR-16-5p) have been utilized in AKI diagnosis . For instance, miRNA-21 and miRNA-200c are also associated with the drug-induced AKI, and accordingly, novel hairpin DNA-fueled nanoflares have been introduced to determine their levels by using target catalytic recycling amplification . Briefly, two hairpin DNA (H1 for miR-21 and H2 for miR-200c) were labeled with FAM and cyanine 5 (Cy5), and they were immobilized on the surface of AuNPs.…”

“…The strategy was to hybridize the double strands, including H1-miR-21 and H2-miR-200c, when miRNA was encountered with nanoflares, and eventually, these interactions resulted in two-colored fluorescence signals. The LOD values of these strategies were 18.1 and 21.1 pM for miRNA-21 and miRNA-200c, respectively …”

A ratiometric nanoprobe for the in vivo bioimaging of hypochlorous acid to detect drug-damaged liver and kidneys

“…As the major organs responsible for the metabolism and excretion of drugs, the liver and kidneys of patients taking drugs for a lengthy period are constantly under threat of suffering from drug toxicity, which could result in chronic nephritis, fatty liver, liver fibrosis, kidney failure, glomerulonephritis, and other diseases, thereby gravely affecting the quality of life. [1][2][3][4][5][6][7][8][9][10][11] Thus, the early assessment of drug-induced liver and kidney damage is essential for the real-time monitoring of the physiological states of the liver and kidneys and for the timely prompting of therapy to prevent further deterioration. [12][13][14][15][16][17] Tissue damage and severe disease can be caused by the irregular over-accumulation of HClO in spite of its role as a vital intracellular and intercellular species in maintaining homeostasis and regulating cell signal transduction.…”