A haemorrhagic fever from the Côte d'lvoire

Teichmann, Dieter; Grobusch, Martin P.; Wesselmann, Hans; Temmesfeld-Wollbrück, Bettina; Breuer, Thomas; Dietel, Manfred; Emmerich, Petra; Schmitz, Herbert; Suttorp, Norbert

doi:10.1016/s0140-6736(99)09233-8

Cited by 42 publications

(22 citation statements)

References 4 publications

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“…Serum samples were from one acute and one convalescent case of Ebola hemorrhagic fever (Gulu, Uganda, October 2000 [3]), two cases of imported Lassa fever (Würzburg, Germany, January 2000 [17], and Leiden, The Netherlands, March 2000 [2]), an acute case of Crimean-Congo hemorrhagic fever (Prizren, Kosovo, May 2000), an imported case of yellow fever (Berlin, Germany, August 1999 [41]), and an imported case of acute secondary dengue fever with hemorrhagic diathesis (Hamburg, Germany, August 2001).…”

Section: Methodsmentioning

confidence: 99%

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Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

et al. 2002

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Viral hemorrhagic fevers (VHFs) are acute infections with high case fatality rates. Important VHF agents are Ebola and Marburg viruses (MBGV/EBOV), Lassa virus (LASV), Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), dengue virus (DENV), and yellow fever virus (YFV). VHFs are clinically difficult to diagnose and to distinguish; a rapid and reliable laboratory diagnosis is required in suspected cases. We have established six one-step, real-time reverse transcription-PCR assays for these pathogens based on the Superscript reverse transcriptase-Platinum Taq polymerase enzyme mixture. Novel primers and/or 5-nuclease detection probes were designed for RVFV, DENV, YFV, and CCHFV by using the latest DNA database entries. PCR products were detected in real time on a LightCycler instrument by using 5-nuclease technology (RVFV, DENV, and YFV) or SybrGreen dye intercalation (MBGV/EBOV, LASV, and CCHFV). The inhibitory effect of SybrGreen on reverse transcription was overcome by initial immobilization of the dye in the reaction capillaries. Universal cycling conditions for SybrGreen and 5-nuclease probe detection were established. Thus, up to three assays could be performed in parallel, facilitating rapid testing for several pathogens. All assays were thoroughly optimized and validated in terms of analytical sensitivity by using in vitro-transcribed RNA. The >95% detection limits as determined by probit regression analysis ranged from 1,545 to 2,835 viral genome equivalents/ml of serum (8.6 to 16 RNA copies per assay). The suitability of the assays was exemplified by detection and quantification of viral RNA in serum samples of VHF patients.

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Section: Methodsmentioning

confidence: 99%

“…In 1999 a patient died from imported yellow fever in Germany (41), and there are several earlier reports on the import of VHF infections into Europe, Japan, or North America (19,42).…”

mentioning

confidence: 99%

Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

et al. 2002

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“…1 Despite the number of travelers, cases in travelers only occur sporadically, [11][12][13][14][15][16] most likely because of international health regulations mandating the use of the vaccine. However, increasing concerns regarding the safety of the vaccine may curtail its use and diminish its effectiveness in outbreak prevention.…”

Section: Introductionmentioning

confidence: 99%

Incubation Periods of Yellow Fever Virus

Johansson¹,

Arana-Vizcarrondo²,

Biggerstaff³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Yellow fever virus is a global health threat due to its endemicity in parts of Africa and South America where human infections occur in residents and travelers. To understand yellow fever dynamics, it is critical to characterize the incubation periods of the virus in vector mosquitoes and humans. Here, we compare four statistical models of the yellow fever incubation periods fitted with historical data. The extrinsic incubation period in the urban vector Aedes aegypti was best characterized with a temperature-dependent Weibull model with a median of 10 days at 25°C (middle 95% = 2.0–37 days). The intrinsic incubation period, fitted with a log-normal model, had a median of 4.3 days (middle 95% = 2.3–8.6 days). These estimates and their associated statistical models provide a quantitative basis to assist in exposure assessments, model potential outbreaks, and evaluate the effectiveness of public health interventions.

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“…A rapid confirmation of the clinical diagnosis is therefore required by methods such as antigen capture enzyme immunoassay and serologic detection of immunoglobulin M. With the development of PCR technology, it has become possible to rapidly test for viruses that cause VHF (4,5,10). We have recently confirmed a case of acute yellow fever with fulminating hepatic failure by reverse transcription-PCR (RT-PCR) (2).…”

Section: False-negative Results Of Pcr Assay With Plasma Of Patients mentioning

confidence: 99%

False-Negative Results of PCR Assay with Plasma of Patients with Severe Viral Hemorrhagic Fever

et al. 2002

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A haemorrhagic fever from the Côte d'lvoire

Cited by 42 publications

References 4 publications

Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

Incubation Periods of Yellow Fever Virus

False-Negative Results of PCR Assay with Plasma of Patients with Severe Viral Hemorrhagic Fever

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