A Haematin-Independent Mutant of Haemophilus influenzae

Butler, L. O.

doi:10.1099/00221287-29-2-189

Cited by 7 publications

(1 citation statement)

References 2 publications

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“…Results similar to those described above in the transformation of pneu-The most important difference recognized between Hemo, Ohilus influenzae and Hemophilus parainfluenzae is that the former requires both hematin and a pyridine nucleotide as growth factors whereas H. parainfluenzae requires a pyridine nucleotide but does not require hematin. Recently Butler (9) has observed what appears to be spontaneous mutation of H. influenzae to H. parainfluenzae, that is the loss of requirement of hematin as a growth factor, Therefore, the assignment of these two representatives of the genus Hemophilus to different species on the basis of hematin requirement may require revision.…”

Section: Reciplcmtmentioning

confidence: 99%

Intraspecies and Interspecies Transformation Reactions in Pneumococcus and Streptococcus

Krauss

MacLeod

1963

The Journal of General Physiology

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The efficiency of transformation of pneumococcus and a strain of viridans streptococcus (strain D) to streptomycin resistance is influenced by the species in which the mutation to resistance occurred, as well as by the species in which the mutated gene has been replicated. Pneumococcus and streptococcus strain D transform in higher frequency with DNA that has been replicated in bacteria of the same species than with DNA from the heterologous species. However, the difference between the frequencies of interspecific and intraspecific transformation is much greater with pneumococcus as receptor than with streptococcus. In addition pneumococcus transforms in higher frequency with wholly homologous (pneumococcal) DNA than with DNA from pneumococci that have replicated the streptococcal Smr gene. Pneumococcus is transformed in lower frequency by wholly heterologous (streptococcal) DNA than by DNA from streptococci that have replicated the pneumococcai Smr gene. Streptococcus behaves similarly in that wholly homologous (streptococcal) DNA transforms it more efficiently than when the transforming fragment contains a pneumococcal moiety. Streptococcus is transformed in the same or lower frequency by wholly heterologous (pneumococcal) DNA than by DNA from pneumococci that have replicated the streptococcal Sm• gene. When erythromycin resistance was used as genetic marker instead of streptomycin resistance, similar results were found.

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Section: Reciplcmtmentioning

confidence: 99%