A gyrovirus infecting a sea bird

Abstract

We characterized the genome of a highly divergent gyrovirus (GyV8) in the spleen and uropygial gland tissues of a diseased northern fulmar (Fulmarus glacialis), a pelagic bird beached in San Francisco, California. No other exogenous viral sequences could be identified using viral metagenomics. The small circular DNA genome shared no significant nucleotide sequence identity, and only 38–42 % amino acid sequence identity in VP1, with any of the previously identified gyroviruses. GyV8 is the first member of the third major phylogenetic clade of this viral genus and the first gyrovirus detected in an avian species other than chicken.

Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation

“…The supernatant was filtered through a 0.45-μm filter (Millipore) to remove bacterium-sized particles. The filtrate was treated with a mixture of enzymes to digest unprotected nucleic acids, and viral nucleic acids were then extracted [11]. Random RT-PCR was performed using first reverse transcription and then primer extension using Klenow DNA polymerase and a primer with degenerate 3′ end (GCCGACTAATGCGTAGTCNNNNNNNNN).…”

Revisiting the taxonomy of the family Circoviridae: establishment of the genus Cyclovirus and removal of the genus Gyrovirus

“…However, other potential members of the genus Gyrovirus have been recently discovered in humans and birds. These novel gyroviruses (GyVs) have been identified in human skin (human gyrovirus (HGyV) [74]) and feces (GyV3 through GyV6; [8,61,62]), chicken serum (avian gyrovirus 2 (AGV2) [66]) and meat (GyV4 and GyV7; [8]) as well as spleen and uropygial gland tissues from sea birds (GyV8; [39]). Since HGyV and AGV2 may represent the same species [61], the genus Gyrovirus should soon be updated to reflect at least eight viral species.…”

A new gyrovirus in human feces

“…At least eight gyrovirus genomes have been described which all include three overlapping ORFs encoding structural protein VP1, non-structural protein VP2, and VP3/Apoptin protein. Gyroviruses can be divided phylogenetically into three clades A (CAV, HGyV/AGV2, GyV3, −6 and −7), B (GyV4, −5), and C (GyV8) [2, 3]. Chicken anemia virus (CAV), the prototype of the Gyrovirus genus, is responsible for severe anemia and immunosuppression in young chickens [4].…”

“…Two additional species, GyV5 and GyV6, were identified in Tunisian diarrheal feces [3]. Recently, GyV7 was reported to infect chicken [12] and GyV8 was found in tissues of a fulmar (sea bird) [2], respectively. Here we describe the genome of a proposed new gyrovirus species (GyV9) found in a human diarrhea sample.…”