2021
DOI: 10.1002/jez.b.23095
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A guide to incubate eggs of Tropidurus lizards under laboratory conditions

Abstract: Studies in Evo-Devo benefit from the use of a variety of organisms, as comparative approaches provide a better understanding of Biodiversity and Evolution. Standardized protocols to incubate eggs and manipulate embryo development enable postulation of additional species as suitable biological systems for research in the field.In the past decades, vertebrate lineages such as Squamata (lizards, snakes, and amphisbaenians) emerged as crucial study systems for addressing topics as diverse as phenotypic evolution a… Show more

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Cited by 2 publications
(8 citation statements)
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“…From the 20 clutches obtained (one from each female), we distributed 80 eggs into small plastic cylindric containers of 145 ml (7.5 cm × 5.0 cm) filled with moist vermiculite. We perforated the lids to enable air renovation and fixed the water potential at -150 cmkPa, replacing eventual evaporated water (based on Steele et al, 2018 and following;Rossigalli-Costa et al, 2021). To avoid genetic bias, we separated the clutches and equally distributed the eggs between two developmental environments: 24 °C (cold, N = 40) and 30 °C (warm, N = 40); we did not consider the thermal regime of 36 °C (overheat) as a third developmental condition because this temperature seems unfeasible for the development of T. catalanensis embryos (see Rossigalli-Costa et al, 2021).…”
Section: Egg Collection and Incubationmentioning
confidence: 99%
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“…From the 20 clutches obtained (one from each female), we distributed 80 eggs into small plastic cylindric containers of 145 ml (7.5 cm × 5.0 cm) filled with moist vermiculite. We perforated the lids to enable air renovation and fixed the water potential at -150 cmkPa, replacing eventual evaporated water (based on Steele et al, 2018 and following;Rossigalli-Costa et al, 2021). To avoid genetic bias, we separated the clutches and equally distributed the eggs between two developmental environments: 24 °C (cold, N = 40) and 30 °C (warm, N = 40); we did not consider the thermal regime of 36 °C (overheat) as a third developmental condition because this temperature seems unfeasible for the development of T. catalanensis embryos (see Rossigalli-Costa et al, 2021).…”
Section: Egg Collection and Incubationmentioning
confidence: 99%
“…We perforated the lids to enable air renovation and fixed the water potential at -150 cmkPa, replacing eventual evaporated water (based on Steele et al, 2018 and following;Rossigalli-Costa et al, 2021). To avoid genetic bias, we separated the clutches and equally distributed the eggs between two developmental environments: 24 °C (cold, N = 40) and 30 °C (warm, N = 40); we did not consider the thermal regime of 36 °C (overheat) as a third developmental condition because this temperature seems unfeasible for the development of T. catalanensis embryos (see Rossigalli-Costa et al, 2021). Due to the lack of information for nest temperatures in T. catalanensis, we chose developmental temperatures within the range of air, substrate and body temperatures recorded for Tropidurus lizards at São Simão and other Brazilian cities where this species is found (see Kohlsdorf and Navas, 2006;Leirião et al, 2019;Maia-Carneiro and Navas, 2021), which also agree with the range of preferred temperatures recorded for Tropidurus lizards in thermal gradients (31 °C to 37 °C, see Kohlsdorf and Navas, 2006).…”
Section: Egg Collection and Incubationmentioning
confidence: 99%
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