A guide to defining and implementing protocols for the welfare assessment of laboratory animals: eleventh report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working Group on Refinement

Hawkins, Penny; Morton, David B.; Burman, Ohp; Dennison, Ngaire; Honess, Paul; Jennings, Maggy; Lane, Sarah; Middleton, V.; Roughan, J. V.; Wells, S.; Westwood, Kerry

doi:10.1258/la.2010.010031

Cited by 192 publications

(193 citation statements)

References 32 publications

(30 reference statements)

Supporting

Mentioning

189

Contrasting

Unclassified

Order By: Relevance

“…The rats were housed at the vivarium of the Instituto de Microbiología y Parasitología Médica (IMPaM), Universidad de Buenos Aires and CONICET. Animal care was in accordance with the guidelines set forth by the National Institutes of Health (22). The use of the rat osteomyelitis model was evaluated by the "Comisión Institucional para el Cuidado y Uso de Animales de Laboratorio" (CICUAL), Facultad de Medicina, Universidad de Buenos Aires, and approved through resolution CD 1938/07.…”

Section: Methodsmentioning

confidence: 99%

Protein Antigens Increase the Protective Efficacy of a Capsule-Based Vaccine against Staphylococcus aureus in a Rat Model of Osteomyelitis

et al. 2014

View full text Add to dashboard Cite

cStaphylococcus aureus is an invasive bacterial pathogen, and antibiotic resistance has impeded adequate control of infections caused by this microbe. Moreover, efforts to prevent human infections with single-component S. aureus vaccines have failed. In this study, we evaluated the protective efficacy in rats of vaccines containing both S. aureus capsular polysaccharides (CPs) and proteins. The serotypes 5 CP (CP5) and 8 CP (CP8) were conjugated to tetanus toxoid and administered to rats alone or together with domain A of clumping factor A (ClfA) or genetically detoxified alpha-toxin (dHla). The vaccines were delivered according to a preventive or a therapeutic regimen, and their protective efficacy was evaluated in a rat model of osteomyelitis. Addition of dHla (but not ClfA) to the CP5 or CP8 vaccine induced reductions in bacterial load and bone morphological changes compared with immunization with either conjugate vaccine alone. Both the prophylactic and therapeutic regimens were protective. Immunization with dHla together with a pneumococcal conjugate vaccine used as a control did not reduce staphylococcal osteomyelitis. The emergence of unencapsulated or small-colony variants during infection was negligible and similar for all of the vaccine groups. In conclusion, addition of dHla to a CP5 or CP8 conjugate vaccine enhanced its efficacy against S. aureus osteomyelitis, indicating that the inclusion of multiple antigens will likely enhance the efficacy of vaccines against both chronic and acute forms of staphylococcal disease.

show abstract

Section: Methodsmentioning

confidence: 99%

Protein Antigens Increase the Protective Efficacy of a Capsule-Based Vaccine against Staphylococcus aureus in a Rat Model of Osteomyelitis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Existen acuerdos y orientaciones sobre las condiciones que deben reunir los bioterios, conforme al tipo de animal que se tiene en ellos (Hawkins et al 2011). La tercera «R» de Russell y Burch (1959), el refinamiento, supone el mejoramiento constante de las técnicas de manejo para reducir el sufrimiento y aumentar el bienestar de los animales que se tienen en cautiverio y se utilizan con fines científicos.…”

Section: Bioterios En Los Laboratorios De Las Escuelasunclassified

El material natural en la Biología escolar. Consideraciones éticas y didácticas sobre las actividades prácticas de laboratorio.

Silva¹

2018

Rev_Eureka_ensen_divulg_cienc

View full text Add to dashboard Cite

Resumen: La enseñanza y el aprendizaje de las ciencias implican necesariamente introducirse en su metodología de trabajo. Las Ciencias Biológicas se caracterizan por utilizar, sobre la base de un marco teórico, la observación y la experimentación con los organismos para la construcción de conocimiento. En la enseñanza de estas ciencias se señalan el valor de una Biología escolar que en sintonía con la ciencia realiza trabajos prácticos de laboratorio (incluyendo observaciones, disecciones y diferentes tipos de experimentaciones con los animales), y la importancia de promover el respeto, la conservación de la vida y el no sufrimiento de los diferentes tipos de organismos que constituyen los ecosistemas. En este trabajo proponemos distintas formas de trabajos prácticos de laboratorio para la enseñanza de la Biología atendiendo al objetivo señalado por las 3 R de Russell y Burch: reducir, remplazar y refinar la observación y experimentación con animales. Proponemos configurar estas actividades como prácticas generadoras. Mediante una planificación didáctica que incluya un interrogatorio oral o escrito (concebido como guía de la observación del material), el trabajo práctico permitirá traer a luz las concepciones que se tienen, ir conectando conocimientos previos y generando explicaciones que permiten la construcción colectiva de conocimiento científico. The natural resource in Biology at school. Ethical and didactic considerations on practical laboratory activitiesAbstract: The teaching and learning of the sciences necessarily imply knowing its methodology in depth. The biological sciences are characterized by using, on the basis of a theoretical framework, the observation and experimentation with organisms for the construction of knowledge. Teaching these sciences consists of putting the value of a school Biology together with the lab experiments conducted in science s (including observation, dissections, and different types of experiments with animals) and the importance of promoting values such as respect, life conservation and no suffering of the ecosystem organisms. In this study we propose different ways of laboratory practical tasks to teach Biology framed within Russell and Burch's 3R objective: to reduce, replace and refine observation and experimentation with animals. We propose to set these activities as generative practices. By means a didactic/methodological planning including a written or oral interview (conceived as a guide to the observation of the material), the practicum will shed light to the preconceptions each individual has, their schemata and at the same time it will generate explanations that allow the collective construction of scientific knowledge. IntroducciónLa enseñanza y el aprendizaje de las ciencias implican necesariamente introducirse en la metodología que las mismas tienen o utilizan para construir conocimientos. Las ciencias naturales se caracterizan por utilizar, sobre la base de un marco teórico, la observación del mundo natural y la experimentación con él, como parte fundamen...

show abstract

“…The challenges of analysing the large amounts of data generated by automated systems (Tecott and Nestler, 2004;Spruijt and DeVisser, 2006) and transforming data into information that is meaningful in terms of animal health and welfare must also be overcome to harness the true power of automated technologies. Finally, because there are practical and technological limitations with automation, homecage technologies should never be used as a substitute to regular clinical monitoring carried out by experienced, compassionate staff (Hawkins, 2002;Hawkins et al, 2011;Hawkins, 2014) instead be used to provide supplemental monitoring.…”

Section: Why Automate the Study Of Behaviour?mentioning

confidence: 99%

The power of automated behavioural homecage technologies in characterizing disease progression in laboratory mice: A review

Richardson

2015

Applied Animal Behaviour Science

View full text Add to dashboard Cite

a b s t r a c tBehavioural changes that occur as animals become sick have been characterized in a number of species and include the less frequent occurrence of 'luxury behaviours' such as playing, grooming and socialization. 'Sickness behaviours' or behavioural changes following exposure to infectious agents, have been particularly well described; animals are typically less active, sleep more, exhibit postural changes and consume less food/water. Disease is frequently induced in laboratory mice to model pathophysiological processes and investigate potential therapies but despite what is known about behavioural changes as animals become sick, behavioural phenotyping of mice involved in disease studies is relatively rare. A detailed understanding of how behaviour changes as mice get sick could be applied to improve welfare of laboratory mice and support the underlying biomedical research. Specifically, characterizing behavioural changes in ill health could help those working with laboratory mice to recognize when refinements should be introduced, when severity limits are being approached and when humane endpoints should be implemented. Understanding how behaviour changes with illness may also help to identify compounds that have a clinical effect as well as when these agents act. There are an increasing number of automated systems to monitor the behaviour of laboratory mice in their homecages incorporating technologies such as the quantification of cage movement, automated video analysis and radiofrequency identification transponders/readers. Mouse models of neurodegenerative diseases particularly Huntington's disease have been well characterized using these systems and behavioural biomarkers of pathology, including changes in the animals' use of environmental enrichment, changes in food/water consumption and alterations in circadian rhythms, are now monitored by laboratories worldwide and used to refine studies and develop therapies. In contrast, automated behavioural technologies have not been used to characterize the behaviour of mice with systemic diseases such as cancer and liver disease. In this review, common behavioural changes that occur in animals with declining health will be discussed with an emphasis on progressive disease studies involving mice. Automated homecage behaviour recording technologies will then be summarized, studies in which these systems have been used to characterize the behaviour of mice with progressive diseases will be reviewed and the potential to apply automated technologies to refine disease studies involving mice will be discussed.

show abstract

A guide to defining and implementing protocols for the welfare assessment of laboratory animals: eleventh report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working Group on Refinement

Cited by 192 publications

References 32 publications

Protein Antigens Increase the Protective Efficacy of a Capsule-Based Vaccine against Staphylococcus aureus in a Rat Model of Osteomyelitis

Protein Antigens Increase the Protective Efficacy of a Capsule-Based Vaccine against Staphylococcus aureus in a Rat Model of Osteomyelitis

El material natural en la Biología escolar. Consideraciones éticas y didácticas sobre las actividades prácticas de laboratorio.

The power of automated behavioural homecage technologies in characterizing disease progression in laboratory mice: A review

Contact Info

Product

Resources

About