A guanine insert in OsBBS1 leads to early leaf senescence and salt stress sensitivity in rice (Oryza sativa L.)

Zeng, Dongdong; Yang, Chengcong; Qin, Ran; Alamin, Md.; Yue, Erkui; Jin, Xiaogang; Shi, Chao

doi:10.1007/s00299-018-2280-y

Cited by 50 publications

(22 citation statements)

References 44 publications

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“…BBS1 expression was upregulated up to 8 h and later down regulated under salt stress. The bbs1 mutant also showed hypersensitivity to salt stress [45]. In our study PTK4/BBS1 expression was upregulated under osmotic stress.…”

Section: Real-time Rt-pcr Analysis Of Expression Of Ptk Genessupporting

confidence: 55%

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Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice

Elangovan

Dalal

Kumar

et al. 2020

Plants

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Tyrosine phosphorylation constitutes up to 5% of the total phophoproteome. However, only limited studies are available on protein tyrosine kinases (PTKs) that catalyze protein tyrosine phosphorylation in plants. In this study, domain analysis of the 27 annotated PTK genes in rice genome led to the identification of 18 PTKs with tyrosine kinase domain. The kinase domain of rice PTKs shared high homology with that of dual specificity kinase BRASSINOSTEROID-INSENSITIVE 1 (BRI1) of Arabidopsis. In phylogenetic analysis, rice PTKs clustered with receptor-like cytoplasmic kinases-VII (RLCKs-VII) of Arabidopsis. mRNAseq analysis using Genevestigator revealed that rice PTKs except PTK9 and PTK16 express at moderate to high level in most tissues. PTK16 expression was highly abundant in panicle at flowering stage. mRNAseq data analysis led to the identification of drought, heat, salt, and submergence stress regulated PTK genes in rice. PTK14 was upregulated under all stresses. qRT-PCR analysis also showed that all PTKs except PTK10 were significantly upregulated in root under osmotic stress. Tissue specificity and abiotic stress mediated differential regulation of PTKs suggest their potential role in development and stress response of rice. The candidate dual specificity PTKs identified in this study paves way for molecular analysis of tyrosine phosphorylation in rice.

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Section: Real-time Rt-pcr Analysis Of Expression Of Ptk Genessupporting

confidence: 55%

“…In our study PTK4/BBS1 expression was upregulated under osmotic stress. Our results suggest that in addition to salt stress [45], PTK4/BBS1 may also confer osmotic stress tolerance to rice.…”

Section: Real-time Rt-pcr Analysis Of Expression Of Ptk Genesmentioning

confidence: 81%

Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice

Elangovan

Dalal

Kumar

et al. 2020

Plants

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“…Instead, as one of the major crop species, and representative of agriculturally most important monocots, rice has been playing an increasing role in fundamental research. For example, Zeng et al () established the function of OsBBS1 , in the context of leaf senescence, and the response to salt stress, by knocking out this gene. Taking a base‐editing approach using nCas9‐PBE, Zong et al () elucidated the role of OsCDC43 in senescence and programmed cell death.…”

Section: Applications Of Targeted Genome Modification By Nhejmentioning

confidence: 99%

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Kumlehn

Pietralla

Hensel

et al. 2018

JIPB

100

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Since the discovery that nucleases of the bacterial CRISPR (clustered regularly interspaced palindromic repeat)‐associated (Cas) system can be used as easily programmable tools for genome engineering, their application massively transformed different areas of plant biology. In this review, we assess the current state of their use for crop breeding to incorporate attractive new agronomical traits into specific cultivars of various crop plants. This can be achieved by the use of Cas9/12 nucleases for double‐strand break induction, resulting in mutations by non‐homologous recombination. Strategies for performing such experiments − from the design of guide RNA to the use of different transformation technologies − are evaluated. Furthermore, we sum up recent developments regarding the use of nuclease‐deficient Cas9/12 proteins, as DNA‐binding moieties for targeting different kinds of enzyme activities to specific sites within the genome. Progress in base deamination, transcriptional induction and transcriptional repression, as well as in imaging in plants, is also discussed. As different Cas9/12 enzymes are at hand, the simultaneous application of various enzyme activities, to multiple genomic sites, is now in reach to redirect plant metabolism in a multifunctional manner and pave the way for a new level of plant synthetic biology.

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“…Leaf senescence is a complex trait involved in various biological processes, including degradation of chloroplasts, degradation of nucleic acids and proteins, and recycling of nutrients (Zeng et al, 2018). Early leaf senescence has a negative effect on rice yield and quality; therefore, it is of great importance to explore the hidden molecular mechanisms.…”

Section: Hda710 May Regulate Leaf Senescence Through Programmed Cell mentioning

confidence: 99%

Over-Expression of HDA710 Delays Leaf Senescence in Rice (Oryza sativa L.)

Zhao

Sheng

Zhao

et al. 2020

Front. Bioeng. Biotechnol.

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Histone deacetylases (HDACs) influence chromatin state and gene expression. Eighteen HDAC genes with important biological functions have been identified in rice. In this study, we surveyed the gene presence frequency of all 18 rice HDAC genes in 3,010 rice accessions. HDA710/OsHDAC2 showed insertion/deletion (InDel) polymorphisms in almost 98.8% japonica accessions but only 1% indica accessions. InDel polymorphism association analysis showed that accessions with partial deletions in HDA710 tended to display early leaf senescence. Further transgenic results confirmed that HDA710 delayed leaf senescence in rice. The over-expression of HDA710 delayed leaf senescence, and the knock-down of HDA710 accelerated leaf senescence. Transcriptome analysis showed that photosynthesis and chlorophyll biosynthesis related genes were up-regulated in HDA710 over-expression lines, while some programmed cell death and disease resistance related genes were down-regulated. Co-expression network analysis with gene expression view revealed that HDA710 was co-expressed with multiple genes, particularly OsGSTU12, which was significantly up-regulated in 35S::HDA710-sense lines. InDels in the promoter region of OsGSTU12 and in the gene region of HDA710 occurred coincidentally among more than 90% accessions, and we identified multiple W-box motifs at the InDel position of OsGSTU12. Over-expression of OsGSTU12 also delayed leaf senescence in rice. Taken together, our results suggest that both HDA710 and OsGSTU12 are involved in regulating the process of leaf senescence in rice.

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A guanine insert in OsBBS1 leads to early leaf senescence and salt stress sensitivity in rice (Oryza sativa L.)

Cited by 50 publications

References 44 publications

Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice

Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Over-Expression of HDA710 Delays Leaf Senescence in Rice (Oryza sativa L.)

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