A growth selection system for sucrose synthases (SuSy): design and test
Gonzalo N. Bidart,
Se Hyeuk,
Tobias Benedikt Alter
et al.
Abstract:High throughput screening (HTS) methods of enzyme variants are essential for the development of robust biocatalysts suited for low impact, industrial scale, biobased synthesis of a myriad of compounds. However, for the majority of enzyme classes, current screening methods have limited throughput, or need expensive substrates in combination with sophisticated setups. Here, we present a straightforward, high throughput selection system that couples sucrose synthase activity to growth. Enabling high throughput sc… Show more
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