A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis

Abstract

The GH (growth hormone) cDNA sequence of the marine silverside fish Odontesthes argentinensis was obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The marine silverside GH cDNA sequence is 928 nucleotides long and was found to encode a polypeptide of 204 amino acids, including a signal peptide of 17 amino acids. The 5' and 3' untranslated regions of the messenger are 109 and 204 nucleotides long, respectively. The deduced GH amino acid sequence was used to infer a phylogenetic tree with GH amino acid sequences from representative species belonging to the Euteleostei Subdivision using the maximum parsimony method. The topology found is according to the major phylogenetic grouping of euteleosts. The results corroborate the hypothesis that atherinids are not related to paracanthopterygians as previously suggested, and show a lack of solid synapomorphies among most of the Acanthopterygii Orders analysed indicating a complex assemblage of fishes in which the phylogenetic tree remains indeterminable.

Molecular profiling of growth hormone in the juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ Epinephelus polyphekadion ♂)

“…represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein". residue (Trp) was observed in hybrid grouper as was figured out in the Hemiramphus brasiliensis, mullet, halfbeak, marine silverside fish, Mugil platanus and Odenthesthes argentinensis, to be one of the physiognomies of growth hormone (Marins et al, 2003;Meire et al, 2006). The growth hormone protein sequences are termed as reflecting the molecular phylogeny of bony fishes (Bernardi et al, 1993;Schneider et al, 1992).…”

Annotation and analysis of expressed sequence tags (ESTs) from Chinese sturgeon (Acipenser sinensis) pituitary cDNA library

“…However, nuclear genes may provide molecular data for more expressive phylogenetic reconstruction. GH, GtH-α subunit and POMC are three polypeptides of fundamental importance for growth regulation in vertebrates (Kawauchi and Sower, 2006), in addition, all three have the critical criteria used in this kind of analysis, for example (i) conservation of the sequence to avoid saturation; (ii) sufficient length; and (iii) a minimal amount of homoplasy (Marins et al, 2003). It would be interesting to do further works to reveal the GH, GtH, POMC molecular mechanisms and its characteristic in this ancient ray-finned fish from an evolutionary point of view.…”

Short Communication Sequence analysis of the growth hormone gene of the South American catfish Rhamdia quelen

“…Structurally, the latter differs from the former by the presence of an intron inserted in the 5th exon (Moriyama et al, 2006). The gh gene has been shown to serve as a natural marker for studies of evolutionary genetics of various fishes because of its sequence conservation, sufficient length and minimal amount of homoplasy (Marins et al, 2003;Chen et al, 2004;Pinheiro et al, 2008). The aim of this study was to annotate the coding sequence of the R. quelen gh gene and perform a gh-based phylogenetic analysis within the Siluriformes family.…”