A gravity-induced flow injection system for surface plasmon resonance biosensor

Zhou, Chao; Mu, Ying; Yang, Mengchao; Song, Qi; Zhang, Ying; Wu, Zhongyu; Xiang, Liancheng; Jin, Wei; Jin, Qinhan

doi:10.1016/j.talanta.2013.03.037

Cited by 15 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14,15 Internal referencing requires selection of a single non-absorbing wavelength for a given sample and then using that wavelength to "re-normalize" the rest of the absorbance curve to the absorbance value at that wavelength. A mathematical replacement for a real-time reference was used to achieve improved baseline stability.…”

Section: Resultsmentioning

confidence: 99%

Thermal energy harvesting near-infrared radiation and accessing low temperatures with plasmonic sensors

2015

View full text Add to dashboard Cite

Near-infrared (NIR) thermal energy harvesting has been demonstrated for gold nanorods (AuNRs), allowing concentration dependent, ppm-level, gas detection of H2, CO, and NO2 at 500 °C without using a white light source. Part-per-million detection capabilities of the gold nanorods are demonstrated with a factor of 11 reduction in collection times in the NIR as compared to measurements made in the visible light region. Decreased collection times are enabled by an increase in S : N ratio, which allowed a demonstration of selectivity through the use of both full spectral and a reduced spectral-based principal component analysis. Furthermore, low temperature thermal imaging spectra have been obtained at sample temperatures ranging from 275-500 °C, showing the possibility of energy harvested gas sensing at lower temperatures. These findings are promising in the area of miniaturizing plasmonic gas sensing technology and integration in areas such as gas turbines.

show abstract

Section: Resultsmentioning

confidence: 99%

Thermal energy harvesting near-infrared radiation and accessing low temperatures with plasmonic sensors

2015

View full text Add to dashboard Cite

show abstract

“…The development of the microfluidic technology has contributed to the emergence of the microfluidic chip which is now commercially mass-produced. The microfluidic chip [20][21][22], also known as lab on a chip, combines and arranges various unit technologies on a small platform to implement all the biochemical laboratory experiments including sampling, sample introduction, sample preparation, reaction, product testing, and so on [23,24]. The fundamental feature of the microfluidic chip is the integration of functional modules.…”

Section: Introductionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Jiang¹,

Jiang

et al. 2020

Preprint

View full text Add to dashboard Cite

KEYWORDS 2 swine disease, microfluidic chips, PCR, multiplex and on-site detection 3 Abstract Background: At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples and generation of pathogenic microorganisms, and does not meet the rapid on-site detection demand. Therefore, development of a technology for rapid and high-throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput detection for swine disease with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation. Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is timeconsuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the positive nucleic acid of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance. Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs port The new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.Corporation for their support of chip design and fabrication and microchip based PCR detection instrument. Authors' contributionsJY designed and directed the study. JS and JY perform the experiment ,collect results and analyze data. YW and BZ draft the first vision of the manuscript. KMW, CG and DXZ collect clinical serum samples. BZ critically revised the manuscripts. All the participants carefully reviewed this vision manuscript. Ethics approval and consent to participate 11Not applicable Consent for publicationNot applicable Competing interest. Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

show abstract

“…The microfluidic chip [18][19][20], also known as lab on a chip, combines and arranges various unit technologies on a small platform to implement all the biochemical laboratory experiments including sampling, sample introduction, sample preparation, reaction, product testing, and so on [21,22]. The fundamental feature of the microfluidic chip is the integration of functional modules.…”

Section: Introductionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Zhou

Jiang²,

et al. 2020

Preprint

View full text Add to dashboard Cite

Background：At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples [32] and generation of pathogenic microorganisms [33], and does not meet the rapid on-site detection demand [34]. Therefore, development of a technology for rapid and high- throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput swine disease detection with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation.Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is time-consuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the clinical samples of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on- site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance.Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs portThe new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.

show abstract

A gravity-induced flow injection system for surface plasmon resonance biosensor

Cited by 15 publications

References 39 publications

Thermal energy harvesting near-infrared radiation and accessing low temperatures with plasmonic sensors

Thermal energy harvesting near-infrared radiation and accessing low temperatures with plasmonic sensors

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Contact Info

Product

Resources

About