A graphene oxide-based fluorescent platform for selective detection of amyloid-β oligomers

Liu, Lin; Xia, Ning; Zhang, Jiebin; Mao, Wenhui; Wu, Yifan; Ge, Xingxing

doi:10.1039/c5ay02018b

Cited by 31 publications

(16 citation statements)

References 58 publications

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“…The limit of detection (LOD), estimated from 3σ of the baseline signals, was approximately 100 pM for the A β oligomers. The obtained LOD of this assay could be comparable to that of other methods such as, ELISA, SERS, MS, SPR, electrochemical and fluorescent sensor 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 . The high sensitivity of the proposed assay might be explained by the highly efficient signal amplification by the aptamer-Au-Th bioconjugate.…”

Section: Resultssupporting

confidence: 53%

“…Electrochemical sensors 7 8 9 , surface plasma resonance sensors 10 11 , and fluorescent sensors 12 13 14 15 16 17 18 19 20 , and surface-enhanced Raman spectroscopy 21 to measure A β oligomers have been designed based on the high binding affinity of peptides, proteins, antibodies and small molecules toward A β oligomers 22 . Although a low detection of these assays was obtained, a drawback of above fabricated assays was their low specificity owing to the interference and nonspecific adsorption from body fluids.…”

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confidence: 99%

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Fabrication of an antibody-aptamer sandwich assay for electrochemical evaluation of levels of β-amyloid oligomers

Zhou

Zhang

Liu

et al. 2016

Sci Rep

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Amyloid β-peptide (Aβ) in its oligomeric form is often considered as the most toxic species in Alzheimer’s disease (AD), and thus Aβ oligomer is a potentially promising candidate biomarker for AD diagnosis. The development of a sensitive and reliable method for monitoring the Aβ oligomer levels in body fluids is an urgent requirement in order to predict the severity and progression at early or preclinical stages of AD. Here, we show a proof of concept for a sensitive and specific detection of Aβ oligomers by an antibody-aptamer sandwich assay. The antibodies of Aβ oligomers and a nanocomposite of gold nanoparticles with aptamer and thionine (aptamer-Au-Th) were used as the recognition element and the detection probe for specifically binding to Aβ oligomers, respectively. The electrochemical signal of Th reduction could provide measurable electrochemical signals, and a low limit of detection (100 pM) was achieved due to the signal amplification by high loading of Th on the gold nanoparticles. The feasibility of the assay was verified by test of Aβ oligomers in artificial cerebrospinal fluid. The proposed strategy presents valuable information related to early diagnosis of AD process.

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Section: Resultssupporting

confidence: 53%

mentioning

confidence: 99%

Fabrication of an antibody-aptamer sandwich assay for electrochemical evaluation of levels of β-amyloid oligomers

Zhou

Zhang

Liu

et al. 2016

Sci Rep

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“…This release induces a measurable increase in fluorescence to indicate the presence of target biomolecules in an analyte. FRET-based aptasensors with nanomolar or even picomolar limits of detection have been developed for many biomolecules of interest, including amyloid β oligomers 15 , single-stranded DNA 16 , insulin 17 , platelet-derived growth factor 18 , thrombin 16 , and a malarial lactate dehydrogenase 19 , 20 . However, most of these sensors are liquid-based, in which solutions of aptamers, quenchers, and an analyte containing the target molecules must be mixed together before detection proceeds.…”

Section: Introductionmentioning

confidence: 99%

Paper-based MoS2 nanosheet-mediated FRET aptasensor for rapid malaria diagnosis

Geldert

Kenry

Lim

2017

Sci Rep

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There has been growing interest in the development of paper-based biosensors because their simplicity and low cost are attractive for point-of-care diagnosis, especially in low-resource areas. However, only a limited range of paper materials – primarily chromatography papers – have been incorporated into diagnostics thus far. Here, we investigate the performance of different types of paper in order to develop an aptamer- and MoS2 nanosheet-based sensor relying on fluorescence resonance energy transfer (FRET) to signal the presence of a target protein. An aptamer which binds to a malarial biomarker, Plasmodium lactate dehydrogenase (pLDH), is chosen for this study, as point-of-care diagnostics would be especially advantageous in low-resource areas, such as those where malaria is prevalent. We observe that of all papers tested, a measurable and specific fluorescence recovery can only be produced on the sensor created with printer paper, while no significant fluorescence recovery is generated on sensors made from other types of paper, including chromatography, lens, and filter papers. Therefore, our findings demonstrate the importance of careful material selection for the development of a paper-based diagnostic test, and suggest that commercially-available products such as printer paper may serve as viable materials to develop cost-effective and simple diagnostics.

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“…Thus, many GO-based fluorescent chem/bio-sensors have been developed for monitoring the enzymatic activities [34,35,36,37,38,39], measuring the levels of various analytes including nucleic acids, proteins, metal ions and small molecules [40,41,42,43,44], and imaging of cells as well as animals [45,46]. Based on the high quenching ability of GO and the specific aptamer–target interaction, several groups have reported the detection of proteins (e.g., thrombin, cyclin A2, amyloid-β oligomers, α-bungarotoxin and antibodies) with the dye-labeled DNA or peptide probes as the recognition elements [47,48,49,50,51]. In a typical detection model, the fluorescence of a dye-labeled probe would be quenched when it was absorbed onto the surface of GO.…”

Section: Introductionmentioning

confidence: 99%

A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin

Xia

Wang

Liu

2016

Sensors

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Human chorionic gonadotropin (hCG) has been regarded as a biomarker for the diagnosis of pregnancy and some cancers. Because the currently used methods (e.g., disposable Point of Care Testing (POCT) device) for hCG detection require the use of many less stable antibodies, simple and cost-effective methods for the sensitive and selective detection of hCG have always been desired. In this work, we have developed a graphene oxide (GO)-based fluorescent platform for the detection of hCG using a fluorescein isothiocyanate (FITC)-labeled hCG-specific binding peptide aptamer (denoted as FITC-PPLRINRHILTR) as the probe, which can be manufactured cheaply and consistently. Specifically, FITC-PPLRINRHILTR adsorbed onto the surface of GO via electrostatic interaction showed a poor fluorescence signal. The specific binding of hCG to FITC-PPLRINRHILTR resulted in the release of the peptide from the GO surface. As a result, an enhanced fluorescence signal was observed. The fluorescence intensity was directly proportional to the hCG concentration in the range of 0.05–20 IU/mL. The detection limit was found to be 20 mIU/mL. The amenability of the strategy to hCG analysis in biological fluids was demonstrated by assaying hCG in the urine samples.

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A graphene oxide-based fluorescent platform for selective detection of amyloid-β oligomers

Abstract: We report a graphene oxide (GO)-based fluorescent platform for selective detection of amyloid-β oligomers (AβOs) based on the strong and specific interaction between AβOs and the PrP(95–110) peptide, a segment of the cellular prion protein.

Cited by 31 publications

References 58 publications

Fabrication of an antibody-aptamer sandwich assay for electrochemical evaluation of levels of β-amyloid oligomers

Fabrication of an antibody-aptamer sandwich assay for electrochemical evaluation of levels of β-amyloid oligomers

Paper-based MoS2 nanosheet-mediated FRET aptasensor for rapid malaria diagnosis

A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin

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