A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis

He, Songnian; Song, Bo; Li, Di; Zhu, Chunyin; Qi, Wenpeng; Wen, Yanqin; Wang, Lihua; Song, Shiping; Fang, Haiping; Chen, Fan

doi:10.1002/adfm.200901639

Cited by 1,319 publications

(898 citation statements)

References 52 publications

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“…DNA hybridisation to immobilised ssDNA probes on CVD graphene could be used to detect single base mismatches [114]. Multiple DNA targets and various mismatch DNA strands were also selectively detected with fluorescence microscopy [115][116][117][118]. Fluorescent dyes attached to ssDNA probes adsorbed to a graphene surface were efficiently quenched by graphene oxide, while after hybridization to complementary or mismatched strands, the fluorescent signals reappeared in the dsDNA.…”

Section: Detection Methods Based On Dna Adsorptionmentioning

confidence: 99%

Graphene nanodevices for DNA sequencing

2016

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Fast, cheap, and reliable DNA sequencing could be one of the most disruptive innovations of this decade as it will pave the way for personalised medicine. In pursuit of such technology, a variety of nanotechnology-based approaches have been explored and established including sequencing with nanopores. Due to its unique structure and properties, graphene provides interesting opportunities for the development of a new sequencing technology. In recent years, a wide range of creative ideas for graphene sequencers have been theoretically proposed and the first experimental demonstrations have begun to appear. Here, we review the different approaches to using graphene nanodevices for DNA sequencing, which involve DNA passing through graphene nanopores, nanogaps, and nanoribbons, and the physisorption of DNA on graphene nanostructures. We discuss the advantages and problems of each of these key techniques, and provide a perspective on the use of graphene in future DNA sequencing technology.

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Section: Detection Methods Based On Dna Adsorptionmentioning

confidence: 99%

Graphene nanodevices for DNA sequencing

2016

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“…Graphene oxide (GO) is a general quencher for a diverse range of fluorophores, including semiconductor quantum dots [87,89,90]. DNA can be adsorbed by GO and also desorb by adding its cDNA to form a duplex [91].…”

Section: Molecular Beaconsmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

190

141

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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“…They are similar in the sense that both effectively adsorb single-stranded (ss) DNA. [17][18][19][20][21] At the same time, both possess strong fluorescence quenching ability and good thermal and electric conductivity. 22,23 Despite these similarities, their surface properties are quite different.…”

Section: Introductionmentioning

confidence: 99%

Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide

et al. 2012

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Understanding the interface between DNA and nanomaterials is crucial for rational design and optimization of biosensors and drug delivery systems. For detection and delivery into cells, where high concentrations of cellular proteins are present, another layer of complexity is added. In this context, we employ polyethylene glycol (PEG) as a model polymer to mimic the excluded volume effect of cellular proteins and test its effect on DNA adsorption and hybridization on gold nanoparticles (AuNPs) and graphene oxide (GO), both of which show great promise for designing intracellular biosensors and drug delivery systems. We show that PEG 20,000 (e.g. 4%) accelerates DNA hybridization to DNAfunctionalized AuNPs by 50-100%, but this enhanced hybridization kinetics has not been observed with free DNA. Therefore, this rate enhancement is attributed to the surface blocking effect by PEG instead of the macromolecular crowding effect. On the other hand, DNA adsorption on citrate-capped AuNP surfaces is impeded even in the presence of a trace level (i.e., parts-per-billion) of PEG, confirming PEG competes with DNA for surface binding sites. Additional insights have been obtained by studying the adsorption of a thiolated DNA and a peptide nucleic acid. In these cases, the steric effects of PEG to impede adsorption are observed. Similar observations have also been made with GO. Therefore, PEG may be used as an effective blocking agent for both hydrophilic AuNP and for GO that also contains hydrophobic domains.

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A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis

Cited by 1,319 publications

References 52 publications

Graphene nanodevices for DNA sequencing

Graphene nanodevices for DNA sequencing

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide

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