A graphene-based fluorescent nanoprobe for silver(i) ions detection by using graphene oxide and a silver-specific oligonucleotide

Wen, Yanqin; Xing, Feifei; He, Songnian; Song, Shiping; Wang, Lihua; Long, Yi‐Tao; Li, Di; Fan, Chunhai

doi:10.1039/b924832c

Cited by 447 publications

(223 citation statements)

References 30 publications

(26 reference statements)

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“…8,18 The EIS technique was used to monitor the charge transfer resistance of the redox couple on the RGO-modified electrode surface after the immobilization of the probe hp-DNA. The introduction of an hp-DNA probe layer sterically hindered the redox couple, Fe(CN) 6 3-and Fe(CN) 6 …”

Section: -mentioning

confidence: 99%

Impedimetric Hg²⁺Detection on Multilayered Reduced Graphene Oxide-Modified Electrode

Park¹,

Kim²,

Haque³

et al. 2012

Bulletin of the Korean Chemical Society

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Recent efforts toward the development of electrochemical biosensors have emphasized the incorporation of nanomaterials, such as carbon nanotubes, metal nanoparticles, and graphene into the electrode. This incorporation, particularly of graphene, significantly improves the sensor sensitivity and selectivity.1,2 Graphene, a single-atom-thick twodimensional sp 2 carbon network material, serves as a good support for biomolecules due to its flat surface, large surface area, and rich π-conjugation structure.3-7 The combination of graphene and DNA has attracted much interest in the biosensor field. Recently, the interaction between hairpin DNA (hp-DNA) and graphene surface have been used to detect single nucleotide polymorphisms (SNP).5,8 Hp-DNA is a secondary DNA structure in which two regions of the same DNA strand form base-pairs in a double helix capped by an unpaired loop. The presence of the unpaired loop permits hp-DNA to readily adsorb onto graphene surfaces.The development of efficient methods for detection of trace Hg 2+ is of significant importance for human health and the environment because Hg 2+ is an extremely toxic heavy metal ion. Numerous strategies including optical and electrochemical methods for the detection of Hg 2+ have been developed.9 Among them, electrochemical impedance spectroscopy (EIS) as a label-free method, is highly attractive for biosensing assay due to its high sensitivity and simplicity. 10Herein, we report an Hg 2+ detection on the reduced graphene oxide (RGO) surfaces modified with hp-DNA using EIS. The RGO-modified electrode surface was prepared by the chemical reduction of layer-by-layer (LbL) assembled graphene oxide (GO) and polyethyleneimine (PEI). The hp-DNA used here as the probe DNA included a loop region containing several thymine bases. The hp-DNA was immobilized onto the RGO-modified indium-tin-oxide (ITO) electrode surface. Hg 2+ may be captured between thyminethymine (T-T) mismatched base pairs to form a duplex structure stabilized by covalent bonds between Hg 2+ and the thymine residues (T-Hg11 which results in removal of hp-DNA from graphene surface, as shown in Figure 1(a).EIS was used to monitor the interfacial changes in the electrode surface after exposure to an Hg 2+ solution. The charge transfer resistance of a redox couple was recorded using the RGO-modified ITO electrode surface after exposure to the probe hp-DNA and the subsequent interactions with Hg 2+ in the buffer solution. To the best of our knowledge, this is the first report of using RGO-modified surface for the electrochemical impedimetric detection of Hg 2+. Furthermore, Hg 2+ detection using hairpin DNA immobilized on the RGOmodified electrode surface has never been reported.GO was observed by atomic force microscopy ( Figure 1(b)), which confirms that individual single-layer GO sheets with a thickness of ~1 nm. GO is readily dispersed in water due to its intrinsic oxygen-containing groups, including phenol, carbonyl, and epoxide groups.12 The presence of these groups enables the easy adsorption ...

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Section: -mentioning

confidence: 99%

Impedimetric Hg²⁺Detection on Multilayered Reduced Graphene Oxide-Modified Electrode

Park¹,

Kim²,

Haque³

et al. 2012

Bulletin of the Korean Chemical Society

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“…While the majority of graphene based sensors employ electrochemical detection methods, a graphene based fluorescent nanoprobe for detection of silver ions has also been developed (163). Fluorescein labeled silver specific oligonucleotides (SSO) were prepared.…”

Section: Carbon Nanotubes and Graphenementioning

confidence: 99%

Nanotechnology Solutions for Global Water Challenges

McGuinness

Garvey

Whelan

et al. 2015

ACS Symposium Series

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The lack of clean and safe drinking water is responsible for more deaths than war, terrorism and weapons of mass destruction combined. This suggests contaminated water poses a significant threat to human health and welfare. In addition, standard water disinfection approaches such as sedimentation, filtration, and chemical or biological degradation are not fully capable of destroying emerging contaminants (e.g. pesticides, pharmaceutical waste products) or certain types of bacteria (e.g. Cryptosporidium parvum). Nanomaterials and nanotechnology based devices can potentially be employed to solve the challenges posed by various contaminants and

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“…Ag + can selectively interact with cytosine bases (C) to form strong and stable C-Ag + -C complexes. [10][11][12][13][14][15][16][17][18] Based on this fact, nanomaterial has been introduced for silver(I) ion detection. For example, Li et al reported a graphene-based fluorescent DNA sensor for silver(I) ions detection.…”

Section: Introductionmentioning

confidence: 99%

Selective and Sensitive Detection of Silver(I) Ion Based on Tetracationic Complex and TGA/GSH Co-capped Quantum Dots as an Effective Fluorescent Sensing Platform

Shu

Xiao

et al. 2017

ANAL. SCI.

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A graphene-based fluorescent nanoprobe for silver(i) ions detection by using graphene oxide and a silver-specific oligonucleotide

Abstract: A fluorescence sensor for Ag(I) ions is developed based on the target-induced conformational change of a silver-specific cytosine-rich oligonucleotide (SSO) and the interactions between the fluorogenic SSO probe and graphene oxide.

Cited by 447 publications

References 30 publications

Impedimetric Hg²⁺Detection on Multilayered Reduced Graphene Oxide-Modified Electrode

Impedimetric Hg²⁺Detection on Multilayered Reduced Graphene Oxide-Modified Electrode

Nanotechnology Solutions for Global Water Challenges

Selective and Sensitive Detection of Silver(I) Ion Based on Tetracationic Complex and TGA/GSH Co-capped Quantum Dots as an Effective Fluorescent Sensing Platform

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A graphene-based fluorescent nanoprobe for silver(i) ions detection by using graphene oxide and a silver-specific oligonucleotide

Abstract: A fluorescence sensor for Ag(I) ions is developed based on the target-induced conformational change of a silver-specific cytosine-rich oligonucleotide (SSO) and the interactions between the fluorogenic SSO probe and graphene oxide.

Cited by 447 publications

References 30 publications

Impedimetric Hg2+Detection on Multilayered Reduced Graphene Oxide-Modified Electrode

Impedimetric Hg2+Detection on Multilayered Reduced Graphene Oxide-Modified Electrode

Nanotechnology Solutions for Global Water Challenges

Selective and Sensitive Detection of Silver(I) Ion Based on Tetracationic Complex and TGA/GSH Co-capped Quantum Dots as an Effective Fluorescent Sensing Platform

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Impedimetric Hg²⁺Detection on Multilayered Reduced Graphene Oxide-Modified Electrode

Impedimetric Hg²⁺Detection on Multilayered Reduced Graphene Oxide-Modified Electrode