A Grapevine TTG2-Like WRKY Transcription Factor Is Involved in Regulating Vacuolar Transport and Flavonoid Biosynthesis

Amato, Alessandra; Cavallini, Erika; Zenoni, Sara; Finezzo, Laura; Begheldo, Maura; Ruperti, Benedetto; Tornielli, Giovanni Battista

doi:10.3389/fpls.2016.01979

Cited by 108 publications

(82 citation statements)

References 89 publications

(166 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression profiles of VvMYB5a and VvMYB5b (Cavallini et al ., ) have been compared with that of VvWRKY26 , which is the homologue of PhPH3 and AtTTG2 and encodes a WRKY transcription factor that can replace the function of PH3 in transgenic ph3 petunia plants (Amato et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…We recently demonstrated that VvWRKY26, the apparent homologue of AtTTG2 and PhPH3 (Wang et al ., ; Verweij et al ., ), can replace the function of the endogenous PH3 in petunia flowers (Amato et al ., ). Expression of VvWRKY26 in ph3 flowers restores the pH of crude petal limb extracts, the colour of the corolla and the wild‐type expression levels for PhPH1 and PhPH5 in petals.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

The MYB5‐driven MBW complex recruits a WRKY factor to enhance the expression of targets involved in vacuolar hyper‐acidification and trafficking in grapevine

et al. 2019

Self Cite

View full text Add to dashboard Cite

† These authors contributed equally to this work. SUMMARYThe accumulation of secondary metabolites and the regulation of tissue acidity contribute to the important traits of grape berry and influence plant performance in response to abiotic and biotic factors. In several plant species a highly conserved MYB-bHLH-WD (MBW) transcriptional regulatory complex controls flavonoid pigment synthesis and transport, and vacuolar acidification in epidermal cells. An additional component, represented by a WRKY-type transcription factor, physically interacts with the complex increasing the expression of some target genes and adding specificity for other targets. Here we investigated the function of MBW(W) complexes involving two MYBs (VvMYB5a and VvMYB5b) and the WRKY factor VvWRKY26 in grapevine (Vitis vinifera L.). Using transgenic grapevine plants we showed that these complexes affected different aspects of morphology, plant development, pH regulation, and pigment accumulation. Transcriptomic analysis identified a core set of putative target genes controlled by VvMYB5a, VvMYB5b, and VvWRKY26 in different tissues. Our data indicated that VvWRKY26 enhances the expression of selected target genes induced by VvMYB5a/b. Among these targets are genes involved in vacuolar hyper-acidification, such as the P-type ATPases VvPH5 and VvPH1, and trafficking, and genes involved in the biosynthesis of flavonoids. In addition, VvWRKY26 is recruited specifically by VvMYB5a, reflecting the functional diversification of VvMYB5a and VvMYB5b. The expression of MBWW complexes in vegetative organs, such as leaves, indicates a possible function of vacuolar hyper-acidification in the repulsion of herbivores and/or in developmental processes, as shown by defects in transgenic grape plants where the complex is inactivated.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

The MYB5‐driven MBW complex recruits a WRKY factor to enhance the expression of targets involved in vacuolar hyper‐acidification and trafficking in grapevine

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Arabidopsis TT8 protein groups in a distinct clade (Consonni, Geuna, Gavazzi, & Tonelli, 1993;Hernandez, Feller, Morohashi, Frame, & Grotewold, 2007) together with the petunia AN1 (Spelt, Quattrocchio, Mol, & Koes, 2000) and the maize IN factor (Burr et al, 1996). The WRKY factor is encoded by a single gene in all studied species (Amato et al, 2017;Johnson, Kolevski, & Smyth, 2002;Verweij et al, 2016). All components of the WMBW complex are essential to efficiently activate anthocyanin synthesis, as shown by the loss (or reduction) of pigmentation in mutants for each of these factors.…”

Section: Introductionmentioning

confidence: 99%

Identification and functional analysis of three new anthocyanin R2R3‐MYB genes in Petunia

et al. 2019

View full text Add to dashboard Cite

We identified three novel members of the R2R3‐ MYB clade of anthocyanin regulators in the genome of the purple flowering Petunia inflata S6 wild accession, and we called them ANTHOCYANIN SYNTHESIS REGULATOR ( ASR ). Two of these genes, ASR 1 and ASR 2, are inactivated by two different single base mutations in their coding sequence. All three of these genes are absent in the white flowering species P. axillaris N and P. parodii , in the red flowering P. exserta , and in several Petunia hybrida lines, including R27 and W115. P. violacea and other P . hybrida lines (M1, V30, and W59) instead harbor functional copies of the ASR genes. Comparative, functional and phylogenic analysis of anthocyanin R2R3‐ MYB genes strongly suggest that the ASR genes cluster is a duplication of the genomic fragment containing the other three R2R3‐ MYB genes with roles in pigmentation that were previously defined, the ANTHOCYANIN 4‐ DEEP PURPLE ‐ PURPLE HAZE ( AN 4 ‐ DPL ‐ PHZ ) cluster. An investigation of the genomic fragments containing anthocyanin MYB s in different Petuni a accessions reveals that massive rearrangements have taken place, resulting in large differences in the regions surrounding these genes, even in closely related species. Yeast two‐hybrid assays showed that the ASR proteins can participate in the WMBW ( WRKY , MYB , B‐ HLH , and WDR ) anthocyanin regulatory complex by interacting with the transcription factors AN 1 and AN 11. All three ASR s can induce anthocyanin synthesis when ectopically expressed in P. hybrida lines, but ASR 1 appeared to be the most effective. The expression patterns of ASR 1 and ASR 2 cover several different petunia tissues with higher expression at early stages of bud development. In contrast, ASR 3 is only weakly expressed in the stigma, ovary, and anther filaments. The characterization of these novel ASR MYB genes completes the picture of the MYB members of the petunia anthocyanin regulatory ...

show abstract

“…With the release of an increasing number of genome sequences, more and more WRKY genes 51 have been identified in a wide range of plant species [9][10][11][12][13][14], suggesting that WRKY proteins have 52 evolved diverse functions with different biochemical properties. To date, many plant WRKY 53 proteins have also been functionally studied in detail, suggesting that they are implicated to 54 modulate seed development, flowering, fruit ripening, senescence and various metabolic processes 55 by binding to W-box element ((C/T)TGAC(T/C)) in the promoter of downstream target genes 56 [15][16][17][18][19]. For example, a WRKY protein is strongly expressed in the endothelium of 57 developing seeds, and plays important role during seed coat development in Arabidopsis [20].…”

mentioning

confidence: 99%

Genome-wide characterization, evolutionary analysis of WRKY genes in Cucurbitaceae species and assessment of its roles in resisting to powdery mildew disease

Gao

ZiGao

Sun

et al. 2018

Preprint

View full text Add to dashboard Cite

16The WRKY proteins constitute a large family of transcription factors that have been known to 17 play a wide range of regulatory roles in multiple biological processes. Over the past few years, 18 many reports have focused on analysis of evolution and biological function of WRKY genes at the 19 whole genome level in different plant species. However, little information is known about WRKY 20 genes in melon (Cucumis melo L.). In the present study, a total of 56 putative WRKY genes were 21 identified in melon, which were randomly distributed on their respective chromosomes. A 22 multiple sequence alignment and phylogenetic analysis using melon, cucumber and watermelon 2 23 predicted WRKY domains indicated that melon WRKY proteins could be classified into three 24 main groups (I-III). Our analysis indicated that no recent duplication events of WRKY genes were 25 detected in melon, and strong purifying selection was observed among the 85 orthologous pairs of 26 Cucurbitaceae species. Expression profiles of CmWRKY derived from RNA-seq data and 27 quantitative RT-PCR (qRT-PCR) analyses showed distinct expression patterns in various tissues, 28 and the expression of 16 CmWRKY were altered following powdery mildew infection in melon. 29Besides, we also found that a total of 24 WRKY genes were co-expressed with 11 VQ family genes 30 in melon. Our comparative genomic analysis provides a foundation for future functional dissection 31 and understanding the evolution of WRKY genes in cucurbitaceae species, and will promote 32 powdery mildew resistance study in melon. 33 Keywords: cucurbitaceae, WRKY, phylogenetic relationship, evolution, powdery mildew 34 disease 35 36 39 number of functions in a wide range of physiological and biochemical processes [1-5]. The 40 WRKY family proteins are defined by a highly conserved domain with approximately 60 amino 41 acid residues in length, which contains one or two highly conserved short peptide WRKYGQK as 42 well as a conserved C2H2-or C2HC-type zinc finger motif [6-8]. The conserved short peptide also 43 consists of various forms, such as WRKYGKK, WRKYDQK, and WRKYDHK. Based on the 44 number of WRKY domains and the type of zinc finger motif, the WRKY family proteins can be 3 45 classified into three groups [7,8]. Group I contains two WRKY motifs and a C2H2 type zinc 46 finger motif (C-X4-5-C-X22-23-H-X1-H). Group II contains one WRKY motif and a C2H2 type 47 59 correlated with different seed size [19]. Besides, VvWRKY26, a TTG2-like homolog protein, 60 controls vacuolar acidification, transport and flavonoid biosynthesis in grapevine, and plays 61 important regulatory roles in fleshy fruit development [17]. 62Recent studies revealed that a large part of WRKY proteins were also involved in response to 63 biotic (bacterial, fungal and viral pathogens) defense and abiotic stress (salinity, drought, heat, 64 cold and osmotic stress) [21]. In many plants, expression of WRKY family genes was significantly 100

show abstract

A Grapevine TTG2-Like WRKY Transcription Factor Is Involved in Regulating Vacuolar Transport and Flavonoid Biosynthesis

Cited by 108 publications

References 89 publications

The MYB5‐driven MBW complex recruits a WRKY factor to enhance the expression of targets involved in vacuolar hyper‐acidification and trafficking in grapevine

The MYB5‐driven MBW complex recruits a WRKY factor to enhance the expression of targets involved in vacuolar hyper‐acidification and trafficking in grapevine

Identification and functional analysis of three new anthocyanin R2R3‐MYB genes in Petunia

Genome-wide characterization, evolutionary analysis of WRKY genes in Cucurbitaceae species and assessment of its roles in resisting to powdery mildew disease

Contact Info

Product

Resources

About