A Grape ASR Protein Involved in Sugar and Abscisic Acid Signaling

Çakır, Birsen; Agasse, Alice; Gaillard, Cécile; Saumonneau, Amélie; Delrot, Serge; Atanassova, Rossitza

doi:10.1105/tpc.013854

Cited by 281 publications

(302 citation statements)

References 53 publications

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“…A number of motifs were identified: GCC box (Brown et al, 2003), MYC recognition (Abe et al, 1997), T/G box (Boter et al, 2004), wound response element (Palm et al, 1990), and abscisic acid response elements (ABREs; Choi et al, 2000;Hattori et al, 2002). Figure 3 also shows the presence of Suc-responsive motifs (SURE1, Suc box 3) shown previously by Cakir et al (2003) to be involved in the transcriptional regulation of VvHT1.…”

Section: Identification Of Aba Regulatory Motifs In the Vvht5 Promotermentioning

confidence: 78%

“…Recognition sequences are as follows: GCC box (GCCGCC; Brown et al, 2003), T/G box (AACGTG; Boter et al, 2004), MYC (CACATG; Abe et al, 1997), ABRE (ACGTGG/TC; Hattori et al, 2002), and wound response element (WRE; AAA/TGTATCC/GA; Palm et al, 1990). Also shown are the Suc-responsive motifs SURE (ATA-GAAA) and Suc box 3 (AAATCA----AA), identified by Cakir et al (2003) to be involved in the transcriptional regulation of VvHT1. The start position of the VvHT5-1K promoter fragment is also indicated.…”

Section: Biotrophic Pathogen Infection and Wounding Induce The Aba Bimentioning

confidence: 99%

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Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

2010

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Biotrophic fungal and oomycete pathogens alter carbohydrate metabolism in infected host tissues. Symptoms such as elevated soluble carbohydrate concentrations and increased invertase activity suggest that a pathogen-induced carbohydrate sink is established. To identify pathogen-induced regulators of carbohydrate sink strength, quantitative real-time polymerase chain reaction was used to measure transcript levels of invertase and hexose transporter genes in biotrophic pathogen-infected grapevine (Vitis vinifera) leaves. The hexose transporter VvHT5 was highly induced in coordination with the cell wall invertase gene VvcwINV by powdery and downy mildew infection. However, similar responses were also observed in response to wounding, suggesting that this is a generalized response to stress. Analysis of the VvHT5 promoter region indicated the presence of multiple abscisic acid (ABA) response elements, suggesting a role for ABA in the transition from source to sink under stress conditions. ABA treatment of grape leaves was found to reproduce the same gene-specific transcriptional changes as observed under biotic and abiotic stress conditions. Furthermore, the key regulatory ABA biosynthetic gene, VvNCED1, was activated under these same stress conditions. VvHT5 promoter::b-glucuronidase-directed expression in transgenic Arabidopsis (Arabidopsis thaliana) was activated by infection with powdery mildew and by ABA treatment, and the expression was closely associated with vascular tissue adjacent to infected regions. Unlike VvHT1 and VvHT3, which appear to be predominantly involved in hexose transport in developing leaves and berries, VvHT5 appears to have a specific role in enhancing sink strength under stress conditions, and this is controlled through ABA. Our data suggest a central role for ABA in the regulation of VvcwINV and VvHT5 expression during the transition from source to sink in response to infection by biotrophic pathogens.

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Section: Identification Of Aba Regulatory Motifs In the Vvht5 Promotermentioning

confidence: 78%

Section: Biotrophic Pathogen Infection and Wounding Induce The Aba Bimentioning

confidence: 99%

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

2010

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“…We suggest that ASR1 might act via its DNA-binding activity to modulate the activity of other gene products, as proposed for example by the fact that histone H1 levels were induced by water stress in plants (Ascenzi & Gantt 1999;Scippa et al 2000). VvMSA, an ASR homolog, was suggested recently to be involved in sugar and ABA signalling in grape fruit (Cakir et al 2003). Alternatively, ASR1 might function as a protective agent against DNA damage at the earlier stages of stress, until other protective proteins or mechanisms that are slower to respond take over, and as mentioned above, we also cannot rule out the possibility that cytosolic ASR1 plays a role in the stress response.…”

mentioning

confidence: 76%

Over‐expression of the water and salt stress‐regulated Asr1 gene confers an increased salt tolerance

Kalifa

Perlson

Gilad

et al. 2004

Plant Cell & Environment

104

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ASR1 is a plant-specific, highly charged, low molecular weight polypeptide. Purified ASR1 was shown to posses sequence specific Zn 2+ + + + -dependent DNA binding activity (Kalifa et al . Biochemical Journal 381, 373-378, 2004). Steady-state levels of tomato Asr1 mRNA and protein are transiently increased following exposure of plants to polyethylene glycol, NaCl or abscisic acid. The biological role of ASR1 could not be deduced from sequence analyses or sequence homologies. Tobacco plants over-expressing tomato ASR1 have a decreased rate of water loss and improved salt tolerance. Upon exposure to salt, ASR1-over-expressing plants accumulate less Na + + + + and proline than wild-type plants, and also results in increased steadystate levels of other gene products under non-stressed plant growth conditions. Therefore, ASR1 is probably involved in the regulation of water-or salt-stress-modulated gene expression.

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“…VvHT1 responsiveness to both signals involves the presence in its promoter of a specific overlapping configuration of the two sugar response cis-elements. This configuration seems a target for the fixation and the action of VvASR (for V. vinifera abscisic acid, stress, ripening-induced) protein (also called VvMSA; Cakir et al, 2003). VvASR expression is strongly up-regulated by the combined effect of sugars and ABA (Cakir et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…This configuration seems a target for the fixation and the action of VvASR (for V. vinifera abscisic acid, stress, ripening-induced) protein (also called VvMSA; Cakir et al, 2003). VvASR expression is strongly up-regulated by the combined effect of sugars and ABA (Cakir et al, 2003). More recently, it was shown that ABA increases invertase activities and amounts during grape development in correlation with soluble sugar concentration (Pan et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

A Sugar-Inducible Protein Kinase, VvSK1, Regulates Hexose Transport and Sugar Accumulation in Grapevine Cells

Lecourieux¹,

Lecourieux²,

Vignault³

et al. 2009

Plant Physiol.

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A Grape ASR Protein Involved in Sugar and Abscisic Acid Signaling

Cited by 281 publications

References 53 publications

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

Over‐expression of the water and salt stress‐regulated Asr1 gene confers an increased salt tolerance

A Sugar-Inducible Protein Kinase, VvSK1, Regulates Hexose Transport and Sugar Accumulation in Grapevine Cells

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