A Granulomatous Colitis of Dogs with Histologic Resemblance to Whipple's Disease

Kruiningen, Herbert J. Van; Montali, Richard J.; Strandberg, John D.; Kirk, Robert Warren

doi:10.1177/030098586500200601

Cited by 44 publications

(115 citation statements)

References 13 publications

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“…Granulomatous colitis of dogs showing microscopic similarity to Whipple's disease has been found, but no specific microbiologic diagnosis was made (58). T. whipplei was not found in the stools of 127 monkeys or apes (43).…”

Section: Nonhuman Sources Of T Whippleimentioning

confidence: 99%

Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

et al. 2017

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SUMMARY Whipple's disease is a rare infectious disease that can be fatal if left untreated. The disease is caused by infection with Tropheryma whipplei, a bacterium that may be more common than was initially assumed. Most patients present with nonspecific symptoms, and as routine cultivation of the bacterium is not feasible, it is difficult to diagnose this infection. On the other hand, due to the generic symptoms, infection with this bacterium is actually quite often in the differential diagnosis. The gold standard for diagnosis used to be periodic acid-Schiff (PAS) staining of duodenal biopsy specimens, but PAS staining has a poor specificity and sensitivity. The development of molecular techniques has resulted in more convenient methods for detecting T. whipplei infections, and this has greatly improved the diagnosis of this often missed infection. In addition, the molecular detection of T. whipplei has resulted in an increase in knowledge about its pathogenicity, and this review gives an overview of the new insights in epidemiology, pathogenesis, clinical manifestations, diagnosis, and treatment of Tropheryma whipplei infections.

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Section: Nonhuman Sources Of T Whippleimentioning

confidence: 99%

Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

et al. 2017

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“…It is against this background that we sought to examine the role of the mucosa-associated flora in granulomatous colitis of Boxer dogs (GCB: also known as histiocytic ulcerative colitis), a severe disease of unknown etiology that typically affects Boxer dogs under four years of age and is characterized by frequent bloody mucoid stools, thickening and ulceration of the colon, anemia, hypoalbuminemia, and weight loss (80). The dominant histological features are loss of colonic epithelium and goblet cells and the accumulation of large numbers of periodic acid-Schiff stain (PAS)-positive macrophages (20,25,77,80).…”

mentioning

confidence: 99%

“…The dominant histological features are loss of colonic epithelium and goblet cells and the accumulation of large numbers of periodic acid-Schiff stain (PAS)-positive macrophages (20,25,77,80). Immunopathological studies describe an increase in immunoglobulin G3 (IgG3) and IgG4 plasma cells, CD 3 T cells, and L1-and major histocompatibility complex II-positive cells (25).…”

mentioning

confidence: 99%

Adherent and InvasiveEscherichia coliIs Associated with Granulomatous Colitis in Boxer Dogs

Simpson¹,

Dogan²,

Rishniw³

et al. 2006

Infect Immun

232

320

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The mucosa-associated microflora is increasingly considered to play a pivotal role in the pathogenesis of inflammatory bowel disease. This study explored the possibility that an abnormal mucosal flora is involved in the etiopathogenesis of granulomatous colitis of Boxer dogs (GCB). Colonic biopsy samples from affected dogs (n ‫؍‬ 13) and controls (n ‫؍‬ 38) were examined by fluorescent in situ hybridization (FISH) with a eubacterial 16S rRNA probe. Culture, 16S ribosomal DNA sequencing, and histochemistry were used to guide subsequent FISH. GCB-associated Escherichia coli isolates were evaluated for their ability to invade and persist in cultured epithelial cells and macrophages as well as for serotype, phylogenetic group, genome size, overall genotype, and presence of virulence genes. Intramucosal gram-negative coccobacilli were present in 100% of GCB samples but not controls. Invasive bacteria hybridized with FISH probes to E. coli. Three of four GCB-associated E. coli isolates adhered to, invaded, and replicated within cultured epithelial cells. Invasion triggered a "splash"-type response, was decreased by cytochalasin D, genistein, colchicine, and wortmannin, and paralleled the behavior of the Crohn's disease-associated strain E. coli LF 82. GCB E. coli and LF 82 were diverse in serotype and overall genotype but similar in phylogeny (B2 and D), in virulence gene profiles (fyuA, irp1, irp2, chuA, fepC, ibeA, kpsMII, iss), in having a larger genome size than commensal E. coli, and in the presence of novel multilocus sequence types. We conclude that GCB is associated with selective intramucosal colonization by E. coli. E. coli strains associated with GCB and Crohn's disease have an adherent and invasive phenotype and novel multilocus sequence types and resemble E. coli associated with extraintestinal disease in phylogeny and virulence gene profile.

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“…GCB has features in common with UC (macroscopic appearance, regional distribution and immunopathology), Whipple's disease (PAS-positive macrophages) and CD (granulomatous inflammation, bacteria within macrophages and may respond to fluroquinolones) in humans but it is not identical to any of these disease syndromes [46][47][48][49]. 16S rDNA libraries generated from GCB dogs are dominated by sequences for Enterobacteriaceae, predominantly E. coli and Shigella and FISH probes recognize invasive E. coli within the colonic mucosa (in the lamina propria and macrophages) of Boxer dogs with GCB but not unaffected controls, and non-GCB colitis.…”

Section: Aiec Are Associated With Intestinal Inflammation In Dogsmentioning

confidence: 99%

Microflora in Crohn’s disease: the emergence of adherent and invasiveEscherichia coli

Dogan¹,

Simpson²

2008

Expert Review of Clinical Immunology

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A Granulomatous Colitis of Dogs with Histologic Resemblance to Whipple's Disease

Cited by 44 publications

References 13 publications

Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

Adherent and InvasiveEscherichia coliIs Associated with Granulomatous Colitis in Boxer Dogs

Microflora in Crohn’s disease: the emergence of adherent and invasiveEscherichia coli

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