A ‘grafting from’ approach to polymer nanorods for pH-triggered intracellular drug delivery

Pelras, Théophile; Duong, Hien T. T.; Kim, Byung J.; Hawkett, Brian S.; Müllner, Markus

doi:10.1016/j.polymer.2017.02.001

Cited by 21 publications

(12 citation statements)

References 42 publications

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“…This correlated well with a previous study using the same polymer backbone. [ 42 ] Similarly, some scission was observed due to strong forces acting on the brushes during the deposition and drying process. [ 40,43 ]…”

Section: Resultsmentioning

confidence: 99%

Stiffness‐Dependent Intracellular Location of Cylindrical Polymer Brushes

Niederberger

Pelras

Manni

et al. 2021

Macromol. Rapid Commun.

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Cylindrical polymer brushes (CPBs) are macromolecules with nanoparticle proportions. Their modular synthesis enables tailoring of their chemical composition as well as the dialing‐up of overall dimensions and physicochemical properties. In this study, two rod‐like poly[(ethylene glycol) methyl ether methacrylate] (PEGMA)‐based CPBs with varying stiffness but otherwise comparable features and functionality, are synthesized. Differences in particle stiffness are assessed using small angle neutron scattering (SANS). It is observed that the fate of the two CPBs within cells is distinctly different. Stiffer CPBs seem to gravitate toward the mitochondria, whereas CPBs with reduced stiffness are present in different intracellular vesicles.

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Section: Resultsmentioning

confidence: 99%

Stiffness‐Dependent Intracellular Location of Cylindrical Polymer Brushes

Niederberger

Pelras

Manni

et al. 2021

Macromol. Rapid Commun.

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“…FLIM has been applied in studies of drug delivery, including intracellular doxorubicin release, adding a new dimension of intracellular information, which was not previously available from biological data [ 10 , 11 , 12 ]. Other studies have been interested in visualizing nanoparticle uptake and subcellular trafficking, particularly for environmentally responsive nanoparticles, which can release their drug cargo in response to a change in environment [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Rapid Fluorescence Lifetime Imaging Microscopy (RapidFLIM) to Examine Dynamics of Nanoparticle Uptake in Live Cells

Ahmed-Cox

Macmillan

Pandžić

et al. 2022

Cells

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A key challenge in nanomedicine stems from the continued need for a systematic understanding of the delivery of nanoparticles in live cells. Complexities in delivery are often influenced by the biophysical characteristics of nanoparticles, where even subtle changes to nanoparticle designs can alter cellular uptake, transport and activity. Close examination of these processes, especially with imaging, offers important insights that can aid in future nanoparticle design or translation. Rapid fluorescence lifetime imaging microscopy (RapidFLIM) is a potentially valuable technology for examining intracellular mechanisms of nanoparticle delivery by directly correlating visual data with changes in the biological environment. To date, applications for this technology in nanoparticle research have not been explored. A PicoQuant RapidFLIM system was used together with commercial silica nanoparticles to follow particle uptake in glioblastoma cells. Importantly, RapidFLIM imaging showed significantly improved image acquisition speeds over traditional FLIM, which enabled the tracking of nanoparticle uptake into subcellular compartments. We determined mean lifetime changes and used this to delineate significant changes in nanoparticle lifetimes (>0.39 ns), which showed clustering of these tracks proximal to both extracellular and nuclear membrane boundaries. These findings demonstrate the ability of RapidFLIM to track, localize and quantify changes in single nanoparticle fluorescence lifetimes and highlight RapidFLIM as a valuable tool for multiparameter visualization and analysis of nanoparticle molecular dynamics in live cells.

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“…In the last decade, there have been increasing research activities in the use of atom transfer radical polymerization (ATRP) to prepare naturally-derived star-like polymers [ 1 – 4 ]. Considering this method, naturally-occurring polymers can be synthesized via three main strategies: “grafting onto” [ 5 – 10 ], “grafting through” [ 11 – 12 ], or “grafting from” [ 10 , 13 – 21 ]. The “grafting from” approach in particular, allows the tailoring of the side chain composition and the introduction of functional groups via polymerization [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of naturally-derived macromolecules through simplified electrochemically mediated ATRP

Chmielarz¹,

Pacześniak²,

Rydel-Ciszek³

et al. 2017

Beilstein J. Org. Chem.

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The flavonoid-based macroinitiator was received for the first time by the transesterification reaction of quercetin with 2-bromoisobutyryl bromide. In accordance with the “grafting from” strategy, a naturally-occurring star-like polymer with a polar 3,3',4',5,6-pentahydroxyflavone core and hydrophobic poly(tert-butyl acrylate) (PtBA) side arms was synthesized via a simplified electrochemically mediated ATRP (seATRP), utilizing only 78 ppm by weight (wt) of a catalytic CuII complex. To demonstrate the possibility of temporal control, seATRP was carried out utilizing a multiple-step potential electrolysis. The rate of the polymerizations was well-controlled by applying optimal potential values during preparative electrolysis to prevent the possibility of intermolecular coupling of the growing polymer arms. This appears to be the first report using on-demand seATRP for the synthesis of QC-(PtBA-Br)5 pseudo-star polymers. The naturally-derived macromolecules showed narrow MWDs (Đ = 1.08–1.11). 1H NMR spectral results confirm the formation of quercetin-based polymers. These new flavonoid-based polymer materials may find applications as antifouling coatings and drug delivery systems.

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A ‘grafting from’ approach to polymer nanorods for pH-triggered intracellular drug delivery

Cited by 21 publications

References 42 publications

Stiffness‐Dependent Intracellular Location of Cylindrical Polymer Brushes

Stiffness‐Dependent Intracellular Location of Cylindrical Polymer Brushes

Application of Rapid Fluorescence Lifetime Imaging Microscopy (RapidFLIM) to Examine Dynamics of Nanoparticle Uptake in Live Cells

Synthesis of naturally-derived macromolecules through simplified electrochemically mediated ATRP

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