A Grading Scheme for the Assessment of Proliferative Lesions of the Mouse Prostate in the TRAMP Model

Suttie, Andrew W.; Nyska, Abraham; Haseman, Joseph K.; Moser, Glenda J.; Hackett, Theleria R.; Goldsworthy, Thomas L.

doi:10.1080/01926230309734

Cited by 36 publications

(76 citation statements)

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“…The predominant growth pattern of the induced lesion was described as microglandular to tubular; cribriform and comedo patterns were not found. Spontaneous proliferative lesions of prostate lobes in mice are uncommon but some transgenic mice show a high incidence (Reznik et al 1981;Shappell et al 2004;Suttie et al 2003 Adenoma: predominance of epithelial component, lack of stromal prominence or expansion Comments: Epithelial-stromal tumor has been described as one of the tumors in the TRAMP mouse .…”

Section: Neoplastic Proliferative Lesions: Accessory Sex Glands Intromentioning

confidence: 99%

Proliferative and Nonproliferative Lesions of the Rat and Mouse Male Reproductive System

Creasy

Bube

Rijk³

et al. 2012

Toxicol Pathol

204

198

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The INHAND Project (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) is a joint initiative of the Societies of Toxicologic Pathology from Europe (ESTP), Great Britain (BSTP), Japan (JSTP), and North America (STP) to develop an internationally accepted nomenclature for proliferative and nonproliferative lesions in laboratory animals. The purpose of this publication is to provide a standardized nomenclature and differential diagnosis for classifying microscopic lesions observed in the male reproductive system of laboratory rats and mice, with color microphotographs illustrating examples of some lesions. The standardized nomenclature presented in this document is also available for society members electronically on the Internet (http://goreni.org). Sources of material included histopathology databases from government, academia, and industrial laboratories throughout the world. Content includes spontaneous and aging lesions as well as lesions induced by exposure to test materials. A widely accepted and utilized international harmonization of nomenclature for lesions of the male reproductive system in laboratory animals will decrease confusion among regulatory and scientific research organizations in different countries and provide a common language to increase and enrich international exchanges of information among toxicologists and pathologists.

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Section: Neoplastic Proliferative Lesions: Accessory Sex Glands Intromentioning

confidence: 99%

Proliferative and Nonproliferative Lesions of the Rat and Mouse Male Reproductive System

Creasy

Bube

Rijk³

et al. 2012

Toxicol Pathol

204

198

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“…As reported by Suttie et al (50), these grade-1 lesions probably represented ductular epithelium in the neck of the anterior gland, not a true proliferative lesion. The incidence and severity (ie, focalness) of the histopathologic findings noted in the TRAMP animals are presented in Tables 2-6.…”

Section: Histopathological Findings and Statistical Evaluationmentioning

confidence: 70%

“…That not all animals had all lobes examined was taken into consideration when applying the statistical analysis. The classification, quantitation, and progression of proliferative epithelial lesions were scored according to the method described by Suttie et al (50). Briefly, progression of proliferative lesions was divided into 6 grades readily identifiable histologically [grade 1-simple flat lesions; grade 2-papillary or cribriform structures, single-layered or with cell piling; grade 3-papillary or cribriform lesions protruding into lumen; grade 4-lesions occupying significant proportion of lumen; grade 5-lesions filling and expanding lumen, or distinct epithelial mass within lumen; grade 6-poorly differentiated epithelial tissue, local invasion, or distant metastasis (This grade was not seen in this experiment.)].…”

Section: Methodsmentioning

confidence: 99%

“…NAO can easily be used in mice because it is water-soluble and stable at high temperatures and lacks toxicity (LD 50 of NAO in mice is 1,500 mg/kg). The efficacy of NAO as an anti-oxidant in both in vitro and in vivo models (dermal, oral, and IP administration) was shown superior to that of the well-known anti-oxidants vitamin E and butylated hydroxytoluene (BHT) (4,24).…”

Section: Introductionmentioning

confidence: 99%

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Slowing Tumorigenic Progression in TRAMP Mice and Prostatic Carcinoma Cell Lines Using Natural Anti-Oxidant from Spinach, NAO—A Comparative Study of Three Anti-Oxidants

et al. 2003

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The TRAMP model and human prostatic cancer (PCA) cell lines DU145 and PC3 are useful for chemopreventive studies. We compared the efficacy of 3 anti-oxidants [a water-soluble natural anti-oxidant, NAO (200 mg/kg), found in spinach leaves; epigallocatechin-3 gallate, EGCG (200 mg/kg), a major green tea polyphenol; and N -acetylcysteine, NAC (125 mg/kg)] plus vehicle in slowing spontaneous tumorigenic progression in TRAMP and wild-type male mice. Sacrifices occurred on weeks 5, 9, and 13. Prostatic histopathology and oxidative-stress blood markers were evaluated. Hyperplasias were ranked by a combination of severity grade and distribution (focal, multifocal, and diffuse). The effectivity of each tested compound in reducing the severity/focalness of hyperplasia varied from lobe to lobe. NAO exerted a significant effect on the dorsal and lateral lobes; NAC, on the anterior and ventral lobes, and EGCG, on the ventral lobe. When the most severe hyperplasia in all 4 lobes of TRAMPs was evaluated, only NAO reduced hyperplasia at weeks 9 and 13. Plasma peroxide levels in TRAMPs were reduced following oral administration of NAO or NAC for 13 weeks; EGCG only slightly reduced these levels. In NAO-treated DU145 and PC3 PCA cells, inhibition of cellular proliferation occurred in a dose-dependent manner, increasing numbers of G1 cells and reducing ROS levels. The anti-oxidative and antiproliferative properties of NAO may explain its efficacy in slowing the spontaneous prostatic carcinogenic process in the TRAMP and its effects in the cell lines.

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“…Alternatively, caloric restriction has been shown to reduce PCa incidence and progression in TRAMP mice [20] [28]. A 20% dietary restriction beginning at 7 weeks of age resulted in significant reductions in PCa incidence at 11 and 20 weeks of age in TRAMP mice [29]. Additionally, TRAMP mice who were intermittently or chronically calorically restricted had a decreased incidence of PCa [20].…”

Section: Concentration (Nm)mentioning

confidence: 99%

Pelleting Diets Impairs TRAMP Prostate Carcinogenesis

Conlon¹,

Wallig²,

Erdman³

2017

FNS

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Diets rich in soy products may reduce the risk of prostate cancer (PCa). Daidzein, the major isoflavone present in soy germ, can be metabolized by the gut microbiota into equol. The effects of daidzein and equol on PCa have not been well studied. The objective of this study was to investigate the effect of feeding 2% soy germ, 92 ppm daidzein, or 88 ppm equol diets on the progression of PCa in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. 3-week old male C57BL/6 X FVB TRAMP mice were weaned from our breeding colony and immediately acclimated to an AIN-93G control diet for one week. At 4 weeks of age, mice (n = 30 per diet group) were randomized to one of four pelleted study diets until 18 weeks of age. Unexpectedly, we did not detect any statistical differences in cancer incidence between diets. We suggest that these results are due to the physical attributes of the pelleted diets in the current study. Mice fed pelleted diets had reduced food intake and significantly decreased body weights (p < 0.001) compared to previous studies. A reduction in food intake is known to reduce cancer incidence in a number of cancer models and is likely to have contributed to the decrease in expected cancer incidence in the current study. In conclusion, we suggest that the hardness of the diets pellets could result in a decreased cancer incidence in TRAMP mice.

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A Grading Scheme for the Assessment of Proliferative Lesions of the Mouse Prostate in the TRAMP Model

Cited by 36 publications

References 0 publications

Proliferative and Nonproliferative Lesions of the Rat and Mouse Male Reproductive System

Proliferative and Nonproliferative Lesions of the Rat and Mouse Male Reproductive System

Slowing Tumorigenic Progression in TRAMP Mice and Prostatic Carcinoma Cell Lines Using Natural Anti-Oxidant from Spinach, NAO—A Comparative Study of Three Anti-Oxidants

Pelleting Diets Impairs TRAMP Prostate Carcinogenesis

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