A gold nanorod-based optical DNA biosensor for the diagnosis of pathogens

Parab, Harshala; Jung, Cheulhee; Lee, Joohyung; Park, Hyun Gyu

doi:10.1016/j.bios.2010.06.067

Cited by 145 publications

(52 citation statements)

References 32 publications

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“…For example, HJ. Parab et al [6] reported an optical biosensor for detecting target DNA, based on GNRs as molecular probes, and X. Wang et al [16] designed a GNR biosensor using localized surface plasmon resonance behavior for detecting the hepatitis B surface antigen.…”

Section: Introductionmentioning

confidence: 99%

“…More research on electrochemical genosensors has focused on spherical nanoparticles [9] than on nanorods. However, GNRs have several advantages over spherical gold nanoparticles, including faster electron transfer [15], improved lightscattering properties [6]. These properties lead to higher sensitivity with lower detection limits.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous techniques, including quartz-crystal microbalance [4], electrochemical [5], optic [6], and surface plasmon resonance [7] methods, have been widely studied for DNA detection. Among them, electrochemical biosensors have become very popular because of their great advantages, including simplicity, rapidity, portability, low cost, and high sensitivity [8].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

et al. 2014

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The purpose of this work was to fabricate an electrochemical DNA biosensor for detecting hepatitis B virus. Gold nanorods (GNRs), which are known for their conductivity, were used to increase surface area and consequently increase the immobilization of single-stranded DNA (ss-DNA) on the modified gold electrode. The GNRs were characterized via transmission electron microscopy. The morphology of the gold electrode before and after modification with GNRs was characterized by scanning electron microscopy. Atomic-force microscopy was used to evaluate the morphology of the GNR electrode surface before and after interaction with ss-DNA. Cyclic voltammetry was used to monitor DNA immobilization and hybridization, using [Co(phen)3](3+) as an electrochemical indicator. The target DNA sequences were quantified at a linear range from 1.0 × 10(-12) to 10.0 × 10(-6) mol L(-1), with a detection limit of 2.0 × 10(-12) mol L(-1) by 3σ. The biosensor had good specificity for distinguishing complementary DNA in the presence of non-complementary and mismatched DNA sequences.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

et al. 2014

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“…Spherical gold nanoparticles (NPs) have been applied to several kinds of analytes in recent years, including DNA, protein, metal ions, and small molecular drugs [11]. Compared to the methods that focus on DNA determination based on sequence-specific target hybridization using spherical gold NPs, the application of nonspherical gold nanostructures has been less well demonstrated, except for a few examples using gold nanorods, though they have several advantages over the spherical gold NPs [12,13].…”

Section: Introductionmentioning

confidence: 99%

A DNA biosensor based on resonance light scattering using unmodified gold bipyramids

Chen

et al. 2012

Microchim Acta

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We report on a novel biosensor for determining sequence-specific DNA. It is based on resonance light scattering (RLS) caused by the aggregation of gold bipyramids. These display localized surface plasmon resonance and can be used as a bioprobe. The absorption spectra and the transmission electron micrographs provide visual evidence of the aggregation of the gold bipyramids in the presence of DNA. The RLS intensity of the gold bipyramids increases with the concentration of the target DNA. The method was successfully applied to the determination of a 30-mer singlestranded oligonucleotide and works over the 0.1-10 nM concentration range.

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“…It has been widely used as a powerful analytical tool in medical diagnostics [1À3], environmental testing [4À6] and food analysis [7]. In principle, a biosensor is fabricated by attaching a bio-receptor such as DNA probes, enzymes, antibodies or cells on the surface of a suitable transducer, which can convert a biological response into an electrical [3,4,8], optical [2] or mechanical [1,7,9] signal. As previously mentioned [10], the signal processor plays an important role in the DNA sensor.…”

Section: Introductionmentioning

confidence: 99%

Novel portable electrical detection system for DNA SENSOR application

Tam

Hong

Hieu

2012

Journal of Experimental Nanoscience

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A novel, portable, electrical detection system was constructed for DNA sensor application to detect DNA hybridisation. The read-out circuit consists of an analogue circuit and a digital circuit. The analogue circuit with an IC MAX038 generates a sine wave with a constant frequency (10 kHz), which serves as the input for the DNA sensor. The DNA sensor consists of active and reference sensors. DNA hybridisation between the DNA probe and the target sequences causes changes in the conductance of the conductive membrane (DNA/MWCNTs) on the sensor surface, which lead to changes in the amplitude of the sine wave from the sensor output compared with that of the reference signal output (input sine wave). We used a digital circuit with a microprocessor (PIC33FJ256MC710) to determine the change in the amplitude of the sine wave signal of the sensor. From these digital data, the difference in the amplitudes of the active and reference sensors was calculated and displayed on the liquid crystal display. Measurement results show that the portable electrical detection system can detect DNA target concentrations as low as 0.16 mM. The detection of the amplified polymerase chain reaction sample and the reproducibility of the DNA sensor results were also determined using the designed readout circuit. The proposed electrical detection system is suitable for DNA sensor application.

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A gold nanorod-based optical DNA biosensor for the diagnosis of pathogens

Cited by 145 publications

References 32 publications

Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

A DNA biosensor based on resonance light scattering using unmodified gold bipyramids

Novel portable electrical detection system for DNA SENSOR application

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