A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

Huang, Yong; Chen, Jia; Shi, Ming; Zhao, Shulin; Chen, Zhengfeng; Liang, Hong

doi:10.1039/c3tb00025g

Cited by 47 publications

(30 citation statements)

References 40 publications

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“…Using ADP as a model inhibitor toward PNK activity, the validity of the as-prepared AuNP/g-C 3 N 4 nanohybrids in screening the inhibition of PNK was evaluated through adding the different-concentration ADP into 50 mU mL -1 PNK (used as an example) with the above-mentioned system. As shown in Figure 5, the photocurrent responses (∆I) 18 gradually decreased with the increasing ADP level (note: The added ADP has no significant effect on the activity of λ exo [33][34][35][36]. Meanwhile, we also observed that addition of 0.6 mM ADP could cause about 50% decrease in ∆I, whilst the PNK activity was completely inhibited when the concentration of ADP was higher than 2.5 mM, indicating the potential application of the developed assay system for the studies of PNK inhibition.…”

Section: Resultsmentioning

confidence: 91%

Plasmonic AuNP/g-C₃N₄Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification

Zhuang

Lai

et al. 2015

ACS Appl. Mater. Interfaces

207

104

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A convenient and feasible photoelectrochemical (PEC) sensing platform based on gold nanoparticles-decorated g-C3N4 nanosheets (AuNP/g-C3N4) was designed for highly sensitive monitoring of T4 polynucleotide kinase (PNK) activity, using DNAzyme-mediated catalytic precipitation amplification. To realize our design, the AuNP/g-C3N4 nanohybrid was initially synthesized through in situ reduction of Au(III) on the g-C3N4 nanosheets, which was utilized for the immobilization of hairpin DNA1 (HP1) on the sensing interface. Thereafter, a target-induced isothermal amplification was automatically carried out on hairpin DNA2 (HP2) in the solution phase through PNK-catalyzed 5'-phosphorylation accompanying formation of numerous trigger DNA fragments, which could induce generation of hemin/G-quadruplex-based DNAzyme on hairpin DNA1. Subsequently, the DNAzyme could catalyze the 4-chloro-1-naphthol (4-CN) oxidation to produce an insoluble precipitation on the AuNP/g-C3N4 surface, thereby resulting in the local alternation of the photocurrent. Experimental results revealed that introduction of AuNP on the g-C3N4 could cause a ∼100% increase in the photocurrent because of surface plasmon resonance-enhanced light harvesting and separation of photogenerated e-/h+ pairs. Under the optimal conditions, the percentage of photocurrent decrement (ΔI/I0, relative to background signal) increased with the increasing PNK activity in a dynamic working range from 2 to 100 mU mL(-1) with a low detection limit (LOD) of 1.0 mU mL(-1). The inhibition effect of adenosine diphosphate also received a good performance in PNK inhibitor screening research, thereby providing a useful scheme for practical use in quantitative PNK activity assay for life science and biological research.

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Section: Resultsmentioning

confidence: 91%

Plasmonic AuNP/g-C₃N₄Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification

Zhuang

Lai

et al. 2015

ACS Appl. Mater. Interfaces

207

104

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“…However, when the fluorescent molecule forms a complex with a protein or something else, it rotates slowly, the FP value increases and the degree of variation depends on the strength of the binding interaction and the size of the complex . Recently, to improve the detection sensitivity, several amplifying strategies that employ proteins , gold nanoparticles (AuNPs) , silica nanoparticles , carbon nanotubes and graphene oxide (GO) have been proposed as FP enhancers for detection of small molecules, proteins and oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

A T7exonuclease‐assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA

et al. 2015

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We report a fluorescence polarization (FP) platform for human immunodeficiency virus (HIV) DNA detection based on T7exonuclease-assisted target recycling amplification with graphene oxide (GO) acting as a FP signal amplifier. In the sensing method, the presence of the target DNA leads to target recycling with the assistance of T7exonuclease, furthermore, the amplification products are absorbed onto the surface of GO, so the all FP values are enhanced by GO. More importantly, this FP sensor exhibits high detection sensitivity; under optimal conditions, the change in FP is linear with the concentration of the target DNA within a concentration range of 50-2000 pmol/L, and the detection limit of this method is as low as 38.6 pmol/L. This FP sensor also exhibits high selectivity, even single-base mismatched DNA can be effectively discriminated from complementary target DNA. Above all, the proposed FP sensor may serve as a general platform for the sensitive assay of disease-related genes.

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“…Biomolecule‐functionalized nanoparticles (NPs), partially due to their biocompatibility and low toxicity, have shown great potential in medical research, such as in sensing, catalysis, imaging, diagnosis, and therapy . Among them, proteins, DNA, and certain small molecules were widely used for synthesis and/or coating of NPs .…”

Section: Introductionmentioning

confidence: 99%

Cross-Linked Proteins with Gold Nanoclusters: A Dual-Purpose pH-Responsive Material for Controllable Cell Imaging and Antibiotic Delivery

Liu

Wang

Hiltunen

et al. 2015

Part. Part. Syst. Charact.

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1wileyonlinelibrary.com www.particle-journal.com www. MaterialsViews.com This report describes the development of a facile method for the synthesis of cross-linked proteins with gold nanoclusters (CP-GNC). The synthesis reaction is completed within 15 min at 97 °C. The synthesized CP-GNC are characterized by using UV-vis absorption, fl uorescence, X-ray photoelectron spectroscopy, and transmission electron microscopy. CP-GNC are approximately 100 nm in diameter and 700 nm in length, whereas AuNCs within the nanorods are approximately 6 nm in size. These materials are highly fl uorescent with quantum yield of 7.2% and can be absorbed onto and release from bacterial cells in a pH-dependent and reversible manner. The recent data show that CP-GNC can be a useful, new tool with potential applications in fl uorescent cell imaging and antibiotic targeting.

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A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

Cited by 47 publications

References 40 publications

Plasmonic AuNP/g-C₃N₄Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification

Plasmonic AuNP/g-C₃N₄Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification

A T7exonuclease‐assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA

Cross-Linked Proteins with Gold Nanoclusters: A Dual-Purpose pH-Responsive Material for Controllable Cell Imaging and Antibiotic Delivery

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A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

Cited by 47 publications

References 40 publications

Plasmonic AuNP/g-C3N4Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification

Plasmonic AuNP/g-C3N4Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification

A T7exonuclease‐assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA

Cross-Linked Proteins with Gold Nanoclusters: A Dual-Purpose pH-Responsive Material for Controllable Cell Imaging and Antibiotic Delivery

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Product

Resources

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Plasmonic AuNP/g-C₃N₄Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification

Plasmonic AuNP/g-C₃N₄Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification